Experiment: KB-half, Pseudo5 supernatant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_21970 and AO356_21975 overlap by 8 nucleotides AO356_21975 and AO356_21980 are separated by 118 nucleotides AO356_21980 and AO356_21985 are separated by 154 nucleotides
AO356_21970: AO356_21970 - LysR family transcriptional regulator, at 4,918,409 to 4,918,822
_21970
AO356_21975: AO356_21975 - antibiotic biosynthesis monooxygenase, at 4,918,815 to 4,919,450
_21975
AO356_21980: AO356_21980 - amidohydrolase, at 4,919,569 to 4,921,407
_21980
AO356_21985: AO356_21985 - hypothetical protein, at 4,921,562 to 4,922,191
_21985
Position (kb)
4919
4920
4921
4922 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4918.743 kb on + strand, within AO356_21970 at 4918.743 kb on + strand, within AO356_21970 at 4918.744 kb on - strand, within AO356_21970 at 4918.744 kb on - strand, within AO356_21970 at 4918.747 kb on + strand, within AO356_21970 at 4918.747 kb on + strand, within AO356_21970 at 4918.748 kb on - strand, within AO356_21970 at 4918.748 kb on - strand, within AO356_21970 at 4918.755 kb on + strand, within AO356_21970 at 4918.756 kb on - strand, within AO356_21970 at 4918.927 kb on - strand, within AO356_21975 at 4918.927 kb on - strand, within AO356_21975 at 4919.310 kb on + strand, within AO356_21975 at 4919.310 kb on + strand, within AO356_21975 at 4919.310 kb on + strand, within AO356_21975 at 4919.310 kb on + strand, within AO356_21975 at 4919.310 kb on + strand, within AO356_21975 at 4919.310 kb on + strand, within AO356_21975 at 4919.311 kb on - strand, within AO356_21975 at 4919.311 kb on - strand, within AO356_21975 at 4919.311 kb on - strand, within AO356_21975 at 4919.361 kb on + strand, within AO356_21975 at 4919.361 kb on + strand, within AO356_21975 at 4919.362 kb on - strand, within AO356_21975 at 4919.362 kb on - strand, within AO356_21975 at 4919.362 kb on - strand, within AO356_21975 at 4919.362 kb on - strand, within AO356_21975 at 4919.449 kb on - strand at 4919.547 kb on + strand at 4919.547 kb on + strand at 4919.548 kb on - strand at 4919.588 kb on + strand at 4919.797 kb on + strand, within AO356_21980 at 4919.797 kb on + strand, within AO356_21980 at 4919.797 kb on + strand, within AO356_21980 at 4919.798 kb on - strand, within AO356_21980 at 4919.798 kb on - strand, within AO356_21980 at 4919.798 kb on - strand, within AO356_21980 at 4919.798 kb on - strand, within AO356_21980 at 4920.004 kb on + strand, within AO356_21980 at 4920.005 kb on - strand, within AO356_21980 at 4920.152 kb on - strand, within AO356_21980 at 4920.190 kb on + strand, within AO356_21980 at 4920.292 kb on + strand, within AO356_21980 at 4920.292 kb on + strand, within AO356_21980 at 4920.293 kb on - strand, within AO356_21980 at 4920.346 kb on + strand, within AO356_21980 at 4920.347 kb on - strand, within AO356_21980 at 4920.347 kb on - strand, within AO356_21980 at 4920.598 kb on + strand, within AO356_21980 at 4920.599 kb on - strand, within AO356_21980 at 4920.599 kb on - strand, within AO356_21980 at 4920.599 kb on - strand, within AO356_21980 at 4920.599 kb on - strand, within AO356_21980 at 4920.779 kb on - strand, within AO356_21980 at 4920.892 kb on + strand, within AO356_21980 at 4920.892 kb on + strand, within AO356_21980 at 4920.892 kb on + strand, within AO356_21980 at 4920.892 kb on + strand, within AO356_21980 at 4920.892 kb on + strand, within AO356_21980 at 4920.893 kb on - strand, within AO356_21980 at 4920.893 kb on - strand, within AO356_21980 at 4920.893 kb on - strand, within AO356_21980 at 4920.893 kb on - strand, within AO356_21980 at 4920.913 kb on + strand, within AO356_21980 at 4920.913 kb on + strand, within AO356_21980 at 4921.162 kb on + strand, within AO356_21980 at 4921.162 kb on + strand, within AO356_21980 at 4921.162 kb on + strand, within AO356_21980 at 4921.163 kb on - strand, within AO356_21980 at 4921.163 kb on - strand, within AO356_21980 at 4921.410 kb on - strand at 4921.410 kb on - strand at 4921.410 kb on - strand at 4921.535 kb on + strand at 4921.535 kb on + strand at 4921.535 kb on + strand at 4921.536 kb on - strand at 4921.538 kb on + strand at 4921.538 kb on + strand at 4921.538 kb on + strand at 4921.580 kb on + strand at 4921.580 kb on + strand at 4921.808 kb on + strand, within AO356_21985 at 4921.808 kb on + strand, within AO356_21985 at 4921.808 kb on + strand, within AO356_21985 at 4921.808 kb on + strand, within AO356_21985 at 4921.808 kb on + strand, within AO356_21985 at 4921.808 kb on + strand, within AO356_21985 at 4921.809 kb on - strand, within AO356_21985 at 4921.809 kb on - strand, within AO356_21985 at 4921.809 kb on - strand, within AO356_21985 at 4921.924 kb on - strand, within AO356_21985 at 4922.147 kb on + strand at 4922.147 kb on + strand at 4922.148 kb on - strand at 4922.153 kb on - strand at 4922.168 kb on + strand at 4922.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB-half, Pseudo5 supernatant remove 4,918,743 + AO356_21970 0.81 -0.3 4,918,743 + AO356_21970 0.81 +0.7 4,918,744 - AO356_21970 0.81 +0.5 4,918,744 - AO356_21970 0.81 +1.3 4,918,747 + AO356_21970 0.82 +1.1 4,918,747 + AO356_21970 0.82 +0.9 4,918,748 - AO356_21970 0.82 +0.3 4,918,748 - AO356_21970 0.82 +0.7 4,918,755 + AO356_21970 0.84 +1.3 4,918,756 - AO356_21970 0.84 -1.3 4,918,927 - AO356_21975 0.18 +1.3 4,918,927 - AO356_21975 0.18 +0.3 4,919,310 + AO356_21975 0.78 -1.7 4,919,310 + AO356_21975 0.78 -0.7 4,919,310 + AO356_21975 0.78 +2.7 4,919,310 + AO356_21975 0.78 +2.0 4,919,310 + AO356_21975 0.78 +0.9 4,919,310 + AO356_21975 0.78 -0.7 4,919,311 - AO356_21975 0.78 +0.4 4,919,311 - AO356_21975 0.78 +0.1 4,919,311 - AO356_21975 0.78 -1.7 4,919,361 + AO356_21975 0.86 +0.3 4,919,361 + AO356_21975 0.86 +0.3 4,919,362 - AO356_21975 0.86 +1.9 4,919,362 - AO356_21975 0.86 +0.0 4,919,362 - AO356_21975 0.86 +0.3 4,919,362 - AO356_21975 0.86 -0.3 4,919,449 - -0.7 4,919,547 + -1.9 4,919,547 + +0.9 4,919,548 - +0.3 4,919,588 + +0.3 4,919,797 + AO356_21980 0.12 -0.1 4,919,797 + AO356_21980 0.12 -1.3 4,919,797 + AO356_21980 0.12 +0.5 4,919,798 - AO356_21980 0.12 -0.7 4,919,798 - AO356_21980 0.12 -0.7 4,919,798 - AO356_21980 0.12 +0.9 4,919,798 - AO356_21980 0.12 +1.3 4,920,004 + AO356_21980 0.24 -0.5 4,920,005 - AO356_21980 0.24 -1.3 4,920,152 - AO356_21980 0.32 -0.1 4,920,190 + AO356_21980 0.34 +0.3 4,920,292 + AO356_21980 0.39 +1.0 4,920,292 + AO356_21980 0.39 +2.3 4,920,293 - AO356_21980 0.39 +0.9 4,920,346 + AO356_21980 0.42 -1.3 4,920,347 - AO356_21980 0.42 +0.0 4,920,347 - AO356_21980 0.42 +0.9 4,920,598 + AO356_21980 0.56 +1.9 4,920,599 - AO356_21980 0.56 +0.4 4,920,599 - AO356_21980 0.56 +0.3 4,920,599 - AO356_21980 0.56 +1.3 4,920,599 - AO356_21980 0.56 -0.7 4,920,779 - AO356_21980 0.66 +1.6 4,920,892 + AO356_21980 0.72 +1.5 4,920,892 + AO356_21980 0.72 -0.1 4,920,892 + AO356_21980 0.72 +0.9 4,920,892 + AO356_21980 0.72 +0.1 4,920,892 + AO356_21980 0.72 -0.9 4,920,893 - AO356_21980 0.72 +0.6 4,920,893 - AO356_21980 0.72 -0.1 4,920,893 - AO356_21980 0.72 -2.3 4,920,893 - AO356_21980 0.72 -0.5 4,920,913 + AO356_21980 0.73 +0.0 4,920,913 + AO356_21980 0.73 +1.2 4,921,162 + AO356_21980 0.87 +1.6 4,921,162 + AO356_21980 0.87 +0.3 4,921,162 + AO356_21980 0.87 -1.3 4,921,163 - AO356_21980 0.87 +1.5 4,921,163 - AO356_21980 0.87 -1.3 4,921,410 - -1.1 4,921,410 - +0.3 4,921,410 - +0.3 4,921,535 + -0.7 4,921,535 + +0.3 4,921,535 + +0.5 4,921,536 - +0.3 4,921,538 + -2.3 4,921,538 + -1.7 4,921,538 + +1.1 4,921,580 + +0.5 4,921,580 + +3.6 4,921,808 + AO356_21985 0.39 +1.3 4,921,808 + AO356_21985 0.39 +0.1 4,921,808 + AO356_21985 0.39 +1.9 4,921,808 + AO356_21985 0.39 +1.2 4,921,808 + AO356_21985 0.39 -0.7 4,921,808 + AO356_21985 0.39 +0.7 4,921,809 - AO356_21985 0.39 +1.1 4,921,809 - AO356_21985 0.39 +0.3 4,921,809 - AO356_21985 0.39 -0.7 4,921,924 - AO356_21985 0.57 -0.3 4,922,147 + +0.2 4,922,147 + +1.6 4,922,148 - +0.5 4,922,153 - +1.9 4,922,168 + +0.3 4,922,190 - -1.7
Or see this region's nucleotide sequence