Experiment: KB-half, Pseudo5 supernatant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_04020 and AO356_04025 are separated by 228 nucleotides AO356_04025 and AO356_04030 overlap by 7 nucleotides AO356_04030 and AO356_04035 are separated by 71 nucleotides
AO356_04020: AO356_04020 - AraC family transcriptional regulator, at 894,006 to 894,755
_04020
AO356_04025: AO356_04025 - hypothetical protein, at 894,984 to 895,874
_04025
AO356_04030: AO356_04030 - selenoprotein, at 895,868 to 896,155
_04030
AO356_04035: AO356_04035 - hypothetical protein, at 896,227 to 898,428
_04035
Position (kb) 894
895
896 Strain fitness (log2 ratio) -2
-1
0
1
2 at 894.025 kb on + strand at 894.033 kb on + strand at 894.033 kb on + strand at 894.033 kb on + strand at 894.154 kb on + strand, within AO356_04020 at 894.395 kb on - strand, within AO356_04020 at 894.588 kb on + strand, within AO356_04020 at 894.588 kb on + strand, within AO356_04020 at 894.588 kb on + strand, within AO356_04020 at 894.588 kb on + strand, within AO356_04020 at 894.589 kb on - strand, within AO356_04020 at 894.589 kb on - strand, within AO356_04020 at 894.791 kb on + strand at 894.791 kb on + strand at 894.791 kb on + strand at 894.791 kb on + strand at 894.792 kb on - strand at 894.810 kb on + strand at 894.810 kb on + strand at 894.811 kb on - strand at 894.811 kb on - strand at 894.811 kb on - strand at 894.814 kb on + strand at 894.815 kb on - strand at 894.877 kb on + strand at 894.878 kb on - strand at 894.878 kb on - strand at 894.878 kb on - strand at 894.891 kb on + strand at 894.892 kb on - strand at 895.504 kb on + strand, within AO356_04025 at 895.505 kb on - strand, within AO356_04025 at 896.062 kb on + strand, within AO356_04030 at 896.063 kb on - strand, within AO356_04030 at 896.121 kb on + strand, within AO356_04030 at 896.122 kb on - strand, within AO356_04030 at 896.122 kb on - strand, within AO356_04030 at 896.122 kb on - strand, within AO356_04030 at 896.122 kb on - strand, within AO356_04030 at 896.188 kb on + strand at 896.188 kb on + strand at 896.188 kb on + strand at 896.189 kb on - strand at 896.189 kb on - strand at 896.189 kb on - strand at 896.190 kb on + strand at 896.190 kb on + strand at 896.190 kb on + strand at 896.190 kb on + strand at 896.190 kb on + strand at 896.190 kb on + strand at 896.190 kb on + strand at 896.190 kb on + strand at 896.191 kb on - strand at 896.191 kb on - strand at 896.191 kb on - strand at 896.191 kb on - strand at 896.222 kb on - strand at 896.222 kb on - strand at 896.424 kb on + strand at 896.425 kb on - strand at 896.425 kb on - strand at 896.449 kb on + strand, within AO356_04035 at 896.449 kb on + strand, within AO356_04035 at 896.449 kb on + strand, within AO356_04035 at 896.450 kb on - strand, within AO356_04035 at 896.450 kb on - strand, within AO356_04035 at 896.450 kb on - strand, within AO356_04035 at 896.450 kb on - strand, within AO356_04035 at 896.461 kb on + strand, within AO356_04035
Per-strain Table
Position Strand Gene LocusTag Fraction KB-half, Pseudo5 supernatant remove 894,025 + +1.2 894,033 + +1.2 894,033 + -0.4 894,033 + -0.4 894,154 + AO356_04020 0.20 +1.2 894,395 - AO356_04020 0.52 -0.2 894,588 + AO356_04020 0.78 +0.2 894,588 + AO356_04020 0.78 -0.4 894,588 + AO356_04020 0.78 +0.2 894,588 + AO356_04020 0.78 -1.0 894,589 - AO356_04020 0.78 +0.2 894,589 - AO356_04020 0.78 +0.6 894,791 + +0.6 894,791 + -0.4 894,791 + +1.4 894,791 + -0.4 894,792 - +0.6 894,810 + +1.6 894,810 + +2.6 894,811 - +0.6 894,811 - +1.2 894,811 - +0.4 894,814 + +0.5 894,815 - +1.2 894,877 + -1.4 894,878 - -0.4 894,878 - +0.6 894,878 - +1.6 894,891 + -0.7 894,892 - +0.6 895,504 + AO356_04025 0.58 -1.7 895,505 - AO356_04025 0.58 -0.4 896,062 + AO356_04030 0.67 -0.2 896,063 - AO356_04030 0.68 -0.4 896,121 + AO356_04030 0.88 +0.6 896,122 - AO356_04030 0.88 -1.4 896,122 - AO356_04030 0.88 +0.6 896,122 - AO356_04030 0.88 +0.9 896,122 - AO356_04030 0.88 +0.7 896,188 + -0.2 896,188 + -0.4 896,188 + +0.2 896,189 - -1.4 896,189 - +1.2 896,189 - +0.3 896,190 + +0.2 896,190 + +1.2 896,190 + +1.9 896,190 + +0.7 896,190 + +2.2 896,190 + +0.6 896,190 + -0.8 896,190 + +0.6 896,191 - +1.6 896,191 - +1.6 896,191 - +1.0 896,191 - +1.2 896,222 - +0.3 896,222 - +2.2 896,424 + -0.4 896,425 - -2.0 896,425 - +0.6 896,449 + AO356_04035 0.10 -1.6 896,449 + AO356_04035 0.10 +0.2 896,449 + AO356_04035 0.10 +1.0 896,450 - AO356_04035 0.10 +0.8 896,450 - AO356_04035 0.10 -0.4 896,450 - AO356_04035 0.10 -0.4 896,450 - AO356_04035 0.10 -0.4 896,461 + AO356_04035 0.11 +0.6
Or see this region's nucleotide sequence