Experiment: KB-half, Pseudo5 supernatant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02675 and AO356_02680 are separated by 24 nucleotides AO356_02680 and AO356_02685 are separated by 2 nucleotides AO356_02685 and AO356_02690 are separated by 271 nucleotides
AO356_02675: AO356_02675 - cell invasion protein, at 587,203 to 588,300
_02675
AO356_02680: AO356_02680 - cell invasion protein SipB, at 588,325 to 590,094
_02680
AO356_02685: AO356_02685 - chaperone protein SicA, at 590,097 to 590,579
_02685
AO356_02690: AO356_02690 - type III secretion system protein SpaS, at 590,851 to 591,885
_02690
Position (kb) 588
589
590
591 Strain fitness (log2 ratio) -2
-1
0
1
2 at 587.636 kb on + strand, within AO356_02675 at 587.636 kb on + strand, within AO356_02675 at 587.636 kb on + strand, within AO356_02675 at 587.637 kb on - strand, within AO356_02675 at 587.716 kb on - strand, within AO356_02675 at 588.288 kb on - strand at 588.291 kb on + strand at 588.291 kb on + strand at 588.291 kb on + strand at 588.292 kb on - strand at 588.292 kb on - strand at 588.292 kb on - strand at 588.303 kb on + strand at 588.303 kb on + strand at 588.303 kb on + strand at 588.304 kb on - strand at 588.304 kb on - strand at 588.307 kb on + strand at 588.307 kb on + strand at 588.308 kb on - strand at 588.426 kb on - strand at 588.426 kb on - strand at 588.481 kb on + strand at 588.907 kb on + strand, within AO356_02680 at 588.907 kb on + strand, within AO356_02680 at 588.907 kb on + strand, within AO356_02680 at 588.908 kb on - strand, within AO356_02680 at 588.922 kb on - strand, within AO356_02680 at 588.922 kb on - strand, within AO356_02680 at 588.923 kb on + strand, within AO356_02680 at 588.923 kb on + strand, within AO356_02680 at 588.923 kb on + strand, within AO356_02680 at 588.923 kb on + strand, within AO356_02680 at 588.924 kb on - strand, within AO356_02680 at 588.924 kb on - strand, within AO356_02680 at 588.924 kb on - strand, within AO356_02680 at 589.190 kb on + strand, within AO356_02680 at 589.541 kb on + strand, within AO356_02680 at 589.698 kb on - strand, within AO356_02680 at 589.698 kb on - strand, within AO356_02680 at 589.885 kb on + strand, within AO356_02680 at 589.885 kb on + strand, within AO356_02680 at 589.885 kb on + strand, within AO356_02680 at 589.885 kb on + strand, within AO356_02680 at 589.885 kb on + strand, within AO356_02680 at 589.886 kb on - strand, within AO356_02680 at 589.886 kb on - strand, within AO356_02680 at 589.886 kb on - strand, within AO356_02680 at 589.886 kb on - strand, within AO356_02680 at 589.934 kb on + strand at 589.934 kb on + strand at 589.935 kb on - strand at 589.935 kb on - strand at 590.083 kb on + strand at 590.084 kb on - strand at 590.084 kb on - strand at 590.143 kb on + strand at 590.143 kb on + strand at 590.143 kb on + strand at 590.144 kb on - strand at 590.194 kb on + strand, within AO356_02685 at 590.195 kb on - strand, within AO356_02685 at 590.195 kb on - strand, within AO356_02685 at 590.254 kb on + strand, within AO356_02685 at 590.255 kb on - strand, within AO356_02685 at 590.303 kb on - strand, within AO356_02685 at 590.377 kb on + strand, within AO356_02685 at 590.377 kb on + strand, within AO356_02685 at 590.378 kb on - strand, within AO356_02685 at 590.378 kb on - strand, within AO356_02685 at 590.386 kb on + strand, within AO356_02685 at 590.386 kb on + strand, within AO356_02685 at 590.386 kb on + strand, within AO356_02685 at 590.440 kb on + strand, within AO356_02685 at 590.440 kb on + strand, within AO356_02685 at 590.440 kb on + strand, within AO356_02685 at 590.440 kb on + strand, within AO356_02685 at 590.440 kb on + strand, within AO356_02685 at 590.461 kb on + strand, within AO356_02685 at 590.461 kb on + strand, within AO356_02685 at 590.461 kb on + strand, within AO356_02685 at 590.462 kb on - strand, within AO356_02685 at 590.710 kb on - strand at 591.003 kb on - strand, within AO356_02690
Per-strain Table
Position Strand Gene LocusTag Fraction KB-half, Pseudo5 supernatant remove 587,636 + AO356_02675 0.39 +0.3 587,636 + AO356_02675 0.39 +0.6 587,636 + AO356_02675 0.39 +0.6 587,637 - AO356_02675 0.40 +2.2 587,716 - AO356_02675 0.47 -0.9 588,288 - +1.9 588,291 + -0.7 588,291 + -0.7 588,291 + -0.1 588,292 - -0.1 588,292 - -0.7 588,292 - +1.3 588,303 + +2.1 588,303 + +0.9 588,303 + -1.7 588,304 - +2.5 588,304 - +1.6 588,307 + -1.3 588,307 + -0.7 588,308 - +0.8 588,426 - +0.3 588,426 - +1.6 588,481 + -0.3 588,907 + AO356_02680 0.33 -2.3 588,907 + AO356_02680 0.33 +1.7 588,907 + AO356_02680 0.33 -0.7 588,908 - AO356_02680 0.33 +0.9 588,922 - AO356_02680 0.34 +1.3 588,922 - AO356_02680 0.34 +0.3 588,923 + AO356_02680 0.34 +0.3 588,923 + AO356_02680 0.34 +2.0 588,923 + AO356_02680 0.34 +0.3 588,923 + AO356_02680 0.34 -1.3 588,924 - AO356_02680 0.34 +2.1 588,924 - AO356_02680 0.34 -0.7 588,924 - AO356_02680 0.34 +0.3 589,190 + AO356_02680 0.49 +0.7 589,541 + AO356_02680 0.69 +0.8 589,698 - AO356_02680 0.78 +1.3 589,698 - AO356_02680 0.78 +0.2 589,885 + AO356_02680 0.88 +1.0 589,885 + AO356_02680 0.88 -1.1 589,885 + AO356_02680 0.88 -0.7 589,885 + AO356_02680 0.88 +2.1 589,885 + AO356_02680 0.88 -1.0 589,886 - AO356_02680 0.88 +0.3 589,886 - AO356_02680 0.88 +0.3 589,886 - AO356_02680 0.88 +1.9 589,886 - AO356_02680 0.88 +1.6 589,934 + -0.1 589,934 + -0.7 589,935 - +1.0 589,935 - +0.3 590,083 + -0.7 590,084 - +0.1 590,084 - -0.1 590,143 + +0.3 590,143 + +0.3 590,143 + -1.1 590,144 - +0.1 590,194 + AO356_02685 0.20 -2.7 590,195 - AO356_02685 0.20 -0.1 590,195 - AO356_02685 0.20 -0.1 590,254 + AO356_02685 0.33 +1.3 590,255 - AO356_02685 0.33 +0.9 590,303 - AO356_02685 0.43 +1.1 590,377 + AO356_02685 0.58 +0.9 590,377 + AO356_02685 0.58 +0.3 590,378 - AO356_02685 0.58 +0.7 590,378 - AO356_02685 0.58 -0.1 590,386 + AO356_02685 0.60 +1.5 590,386 + AO356_02685 0.60 -1.1 590,386 + AO356_02685 0.60 +0.3 590,440 + AO356_02685 0.71 -0.7 590,440 + AO356_02685 0.71 -1.0 590,440 + AO356_02685 0.71 +0.3 590,440 + AO356_02685 0.71 -1.5 590,440 + AO356_02685 0.71 -0.2 590,461 + AO356_02685 0.75 -0.7 590,461 + AO356_02685 0.75 +0.3 590,461 + AO356_02685 0.75 -1.0 590,462 - AO356_02685 0.76 +0.2 590,710 - -0.1 591,003 - AO356_02690 0.15 +1.9
Or see this region's nucleotide sequence