Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3131 and Rru_A3132 are separated by 633 nucleotides Rru_A3132 and Rru_A3133 are separated by 60 nucleotides Rru_A3133 and Rru_A3134 overlap by 4 nucleotides
Rru_A3131: Rru_A3131 - Secretion protein HlyD (NCBI), at 3,609,912 to 3,610,946
_A3131
Rru_A3132: Rru_A3132 - Putative diguanylate cyclase (GGDEF domain) with GAF sensor domain (NCBI), at 3,611,580 to 3,613,061
_A3132
Rru_A3133: Rru_A3133 - hypothetical protein (NCBI), at 3,613,122 to 3,613,337
_A3133
Rru_A3134: Rru_A3134 - AMP-dependent synthetase and ligase (NCBI), at 3,613,334 to 3,614,950
_A3134
Position (kb)
3611
3612
3613
3614 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3610.619 kb on - strand, within Rru_A3131 at 3610.858 kb on + strand at 3610.858 kb on + strand at 3610.864 kb on + strand at 3610.864 kb on + strand at 3610.864 kb on + strand at 3610.864 kb on + strand at 3610.879 kb on + strand at 3610.880 kb on - strand at 3610.880 kb on - strand at 3610.880 kb on - strand at 3610.880 kb on - strand at 3610.933 kb on + strand at 3610.933 kb on + strand at 3610.934 kb on - strand at 3610.986 kb on + strand at 3611.064 kb on - strand at 3611.148 kb on - strand at 3611.227 kb on + strand at 3611.227 kb on + strand at 3611.227 kb on + strand at 3611.228 kb on - strand at 3611.229 kb on + strand at 3611.229 kb on + strand at 3611.230 kb on - strand at 3611.230 kb on - strand at 3611.230 kb on - strand at 3611.242 kb on - strand at 3611.771 kb on + strand, within Rru_A3132 at 3611.771 kb on + strand, within Rru_A3132 at 3611.771 kb on + strand, within Rru_A3132 at 3611.772 kb on - strand, within Rru_A3132 at 3611.772 kb on - strand, within Rru_A3132 at 3611.847 kb on + strand, within Rru_A3132 at 3611.848 kb on - strand, within Rru_A3132 at 3612.068 kb on + strand, within Rru_A3132 at 3612.068 kb on + strand, within Rru_A3132 at 3612.377 kb on + strand, within Rru_A3132 at 3612.377 kb on + strand, within Rru_A3132 at 3612.377 kb on + strand, within Rru_A3132 at 3612.378 kb on - strand, within Rru_A3132 at 3612.378 kb on - strand, within Rru_A3132 at 3612.378 kb on - strand, within Rru_A3132 at 3612.378 kb on - strand, within Rru_A3132 at 3612.378 kb on - strand, within Rru_A3132 at 3612.378 kb on - strand, within Rru_A3132 at 3612.638 kb on + strand, within Rru_A3132 at 3612.638 kb on + strand, within Rru_A3132 at 3612.638 kb on + strand, within Rru_A3132 at 3612.638 kb on + strand, within Rru_A3132 at 3612.638 kb on + strand, within Rru_A3132 at 3612.638 kb on + strand, within Rru_A3132 at 3612.638 kb on + strand, within Rru_A3132 at 3612.638 kb on + strand, within Rru_A3132 at 3612.639 kb on - strand, within Rru_A3132 at 3612.639 kb on - strand, within Rru_A3132 at 3612.639 kb on - strand, within Rru_A3132 at 3612.639 kb on - strand, within Rru_A3132 at 3612.639 kb on - strand, within Rru_A3132 at 3612.639 kb on - strand, within Rru_A3132 at 3612.789 kb on + strand, within Rru_A3132 at 3613.065 kb on + strand at 3613.065 kb on + strand at 3613.065 kb on + strand at 3613.065 kb on + strand at 3613.066 kb on - strand at 3613.066 kb on - strand at 3613.066 kb on - strand at 3613.066 kb on - strand at 3613.066 kb on - strand at 3613.173 kb on + strand, within Rru_A3133 at 3613.451 kb on + strand at 3613.452 kb on - strand at 3613.452 kb on - strand at 3613.452 kb on - strand at 3613.452 kb on - strand at 3613.643 kb on - strand, within Rru_A3134 at 3613.708 kb on + strand, within Rru_A3134 at 3613.709 kb on - strand, within Rru_A3134 at 3613.709 kb on - strand, within Rru_A3134 at 3613.875 kb on - strand, within Rru_A3134 at 3613.875 kb on - strand, within Rru_A3134 at 3613.876 kb on + strand, within Rru_A3134
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 3,610,619 - Rru_A3131 0.68 +0.6 3,610,858 + +1.3 3,610,858 + +1.6 3,610,864 + -0.2 3,610,864 + +0.5 3,610,864 + +0.5 3,610,864 + +0.4 3,610,879 + -2.8 3,610,880 - +0.1 3,610,880 - +0.9 3,610,880 - -3.4 3,610,880 - -1.6 3,610,933 + -1.7 3,610,933 + -0.3 3,610,934 - -0.1 3,610,986 + -1.4 3,611,064 - -0.1 3,611,148 - -0.1 3,611,227 + -0.5 3,611,227 + +0.2 3,611,227 + -0.2 3,611,228 - -3.7 3,611,229 + +0.9 3,611,229 + -1.0 3,611,230 - +0.1 3,611,230 - +1.3 3,611,230 - -0.5 3,611,242 - +0.1 3,611,771 + Rru_A3132 0.13 +1.2 3,611,771 + Rru_A3132 0.13 +0.6 3,611,771 + Rru_A3132 0.13 -0.3 3,611,772 - Rru_A3132 0.13 -0.3 3,611,772 - Rru_A3132 0.13 +0.5 3,611,847 + Rru_A3132 0.18 +1.1 3,611,848 - Rru_A3132 0.18 -0.2 3,612,068 + Rru_A3132 0.33 +0.5 3,612,068 + Rru_A3132 0.33 -0.0 3,612,377 + Rru_A3132 0.54 +0.1 3,612,377 + Rru_A3132 0.54 +0.3 3,612,377 + Rru_A3132 0.54 +0.8 3,612,378 - Rru_A3132 0.54 +0.2 3,612,378 - Rru_A3132 0.54 +0.1 3,612,378 - Rru_A3132 0.54 +2.1 3,612,378 - Rru_A3132 0.54 +0.3 3,612,378 - Rru_A3132 0.54 +0.4 3,612,378 - Rru_A3132 0.54 +0.1 3,612,638 + Rru_A3132 0.71 -0.0 3,612,638 + Rru_A3132 0.71 +1.0 3,612,638 + Rru_A3132 0.71 -0.5 3,612,638 + Rru_A3132 0.71 +1.1 3,612,638 + Rru_A3132 0.71 +1.5 3,612,638 + Rru_A3132 0.71 -3.0 3,612,638 + Rru_A3132 0.71 +1.8 3,612,638 + Rru_A3132 0.71 +0.4 3,612,639 - Rru_A3132 0.71 +1.9 3,612,639 - Rru_A3132 0.71 -0.0 3,612,639 - Rru_A3132 0.71 -3.1 3,612,639 - Rru_A3132 0.71 +5.0 3,612,639 - Rru_A3132 0.71 -0.6 3,612,639 - Rru_A3132 0.71 -1.4 3,612,789 + Rru_A3132 0.82 -0.7 3,613,065 + +1.2 3,613,065 + -1.8 3,613,065 + -0.8 3,613,065 + +0.4 3,613,066 - +0.4 3,613,066 - -0.1 3,613,066 - -1.2 3,613,066 - +0.2 3,613,066 - -0.9 3,613,173 + Rru_A3133 0.24 -2.9 3,613,451 + -0.7 3,613,452 - -1.2 3,613,452 - +0.1 3,613,452 - -0.6 3,613,452 - -2.4 3,613,643 - Rru_A3134 0.19 -1.4 3,613,708 + Rru_A3134 0.23 -0.1 3,613,709 - Rru_A3134 0.23 +0.2 3,613,709 - Rru_A3134 0.23 -0.4 3,613,875 - Rru_A3134 0.33 +1.0 3,613,875 - Rru_A3134 0.33 -0.1 3,613,876 + Rru_A3134 0.34 +0.4
Or see this region's nucleotide sequence