Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2167 and Rru_A2168 overlap by 4 nucleotides Rru_A2168 and Rru_A2169 are separated by 99 nucleotides Rru_A2169 and Rru_A2170 are separated by 399 nucleotides
Rru_A2167: Rru_A2167 - Phosphoribosylformylglycinamidine cyclo-ligase (NCBI), at 2,516,259 to 2,517,425
_A2167
Rru_A2168: Rru_A2168 - Phosphoribosylglycinamide formyltransferase (NCBI), at 2,517,422 to 2,518,096
_A2168
Rru_A2169: Rru_A2169 - Nucleoside-diphosphate kinase (NCBI), at 2,518,196 to 2,518,618
_A2169
Rru_A2170: Rru_A2170 - ABC transporter component (NCBI), at 2,519,018 to 2,520,955
_A2170
Position (kb)
2517
2518
2519 Strain fitness (log2 ratio)
-2
-1
0
1 at 2516.537 kb on + strand, within Rru_A2167 at 2516.679 kb on + strand, within Rru_A2167 at 2516.802 kb on + strand, within Rru_A2167 at 2516.802 kb on + strand, within Rru_A2167 at 2516.817 kb on + strand, within Rru_A2167 at 2517.048 kb on + strand, within Rru_A2167 at 2517.104 kb on + strand, within Rru_A2167 at 2518.197 kb on + strand at 2518.662 kb on - strand at 2518.662 kb on - strand at 2518.662 kb on - strand at 2518.947 kb on - strand at 2518.978 kb on + strand at 2518.979 kb on - strand at 2518.979 kb on - strand at 2518.979 kb on - strand at 2519.042 kb on + strand at 2519.086 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 2,516,537 + Rru_A2167 0.24 -1.8 2,516,679 + Rru_A2167 0.36 -2.6 2,516,802 + Rru_A2167 0.47 -1.9 2,516,802 + Rru_A2167 0.47 -2.5 2,516,817 + Rru_A2167 0.48 -1.5 2,517,048 + Rru_A2167 0.68 -1.6 2,517,104 + Rru_A2167 0.72 -2.3 2,518,197 + -0.3 2,518,662 - -1.4 2,518,662 - -0.3 2,518,662 - -0.2 2,518,947 - +0.4 2,518,978 + -0.4 2,518,979 - +1.5 2,518,979 - -0.1 2,518,979 - +0.5 2,519,042 + -1.6 2,519,086 + -0.7
Or see this region's nucleotide sequence