Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1982 and Rru_A1983 are separated by 105 nucleotides Rru_A1983 and Rru_A1984 are separated by 211 nucleotides Rru_A1984 and Rru_A1985 overlap by 4 nucleotides Rru_A1985 and Rru_A1986 are separated by 130 nucleotides
Rru_A1982: Rru_A1982 - SEC-C domain protein (NCBI), at 2,293,889 to 2,294,389
_A1982
Rru_A1983: Rru_A1983 - hypothetical protein (NCBI), at 2,294,495 to 2,294,701
_A1983
Rru_A1984: Rru_A1984 - nitrogen fixation-related protein (NCBI), at 2,294,913 to 2,295,272
_A1984
Rru_A1985: Rru_A1985 - hypothetical protein (NCBI), at 2,295,269 to 2,295,700
_A1985
Rru_A1986: Rru_A1986 - Protein of unknown function DUF1526 (NCBI), at 2,295,831 to 2,296,550
_A1986
Position (kb)
2294
2295
2296 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2293.917 kb on + strand at 2293.917 kb on + strand at 2293.917 kb on + strand at 2293.918 kb on - strand at 2293.918 kb on - strand at 2294.271 kb on + strand, within Rru_A1982 at 2294.272 kb on - strand, within Rru_A1982 at 2294.272 kb on - strand, within Rru_A1982 at 2294.392 kb on + strand at 2294.397 kb on + strand at 2294.397 kb on + strand at 2294.397 kb on + strand at 2294.397 kb on + strand at 2294.398 kb on - strand at 2294.398 kb on - strand at 2294.472 kb on + strand at 2294.472 kb on + strand at 2294.473 kb on - strand at 2294.532 kb on + strand, within Rru_A1983 at 2294.532 kb on + strand, within Rru_A1983 at 2294.533 kb on - strand, within Rru_A1983 at 2294.533 kb on - strand, within Rru_A1983 at 2294.533 kb on - strand, within Rru_A1983 at 2294.667 kb on + strand, within Rru_A1983 at 2294.668 kb on - strand, within Rru_A1983 at 2294.668 kb on - strand, within Rru_A1983 at 2294.668 kb on - strand, within Rru_A1983 at 2294.668 kb on - strand, within Rru_A1983 at 2294.727 kb on + strand at 2294.727 kb on + strand at 2294.727 kb on + strand at 2294.728 kb on - strand at 2294.728 kb on - strand at 2294.728 kb on - strand at 2295.090 kb on + strand, within Rru_A1984 at 2295.090 kb on + strand, within Rru_A1984 at 2295.091 kb on - strand, within Rru_A1984 at 2295.091 kb on - strand, within Rru_A1984 at 2295.372 kb on - strand, within Rru_A1985 at 2295.372 kb on - strand, within Rru_A1985 at 2295.413 kb on + strand, within Rru_A1985 at 2295.413 kb on + strand, within Rru_A1985 at 2295.414 kb on - strand, within Rru_A1985 at 2295.414 kb on - strand, within Rru_A1985 at 2295.414 kb on - strand, within Rru_A1985 at 2295.415 kb on + strand, within Rru_A1985 at 2295.415 kb on + strand, within Rru_A1985 at 2295.416 kb on - strand, within Rru_A1985 at 2295.416 kb on - strand, within Rru_A1985 at 2295.416 kb on - strand, within Rru_A1985 at 2295.781 kb on - strand at 2295.807 kb on - strand at 2295.832 kb on + strand at 2295.832 kb on + strand at 2295.832 kb on + strand at 2295.832 kb on + strand at 2295.832 kb on + strand at 2295.833 kb on - strand at 2295.833 kb on - strand at 2295.833 kb on - strand at 2295.952 kb on + strand, within Rru_A1986 at 2295.952 kb on + strand, within Rru_A1986 at 2296.018 kb on - strand, within Rru_A1986 at 2296.018 kb on - strand, within Rru_A1986 at 2296.018 kb on - strand, within Rru_A1986 at 2296.018 kb on - strand, within Rru_A1986 at 2296.021 kb on + strand, within Rru_A1986 at 2296.021 kb on + strand, within Rru_A1986 at 2296.047 kb on - strand, within Rru_A1986 at 2296.047 kb on - strand, within Rru_A1986 at 2296.047 kb on - strand, within Rru_A1986 at 2296.047 kb on - strand, within Rru_A1986 at 2296.047 kb on - strand, within Rru_A1986 at 2296.108 kb on + strand, within Rru_A1986 at 2296.109 kb on - strand, within Rru_A1986 at 2296.190 kb on + strand, within Rru_A1986 at 2296.191 kb on - strand, within Rru_A1986 at 2296.191 kb on - strand, within Rru_A1986 at 2296.211 kb on - strand, within Rru_A1986 at 2296.211 kb on - strand, within Rru_A1986
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 2,293,917 + -2.0 2,293,917 + -1.6 2,293,917 + +1.0 2,293,918 - +0.9 2,293,918 - +1.5 2,294,271 + Rru_A1982 0.76 -3.4 2,294,272 - Rru_A1982 0.76 -0.8 2,294,272 - Rru_A1982 0.76 +0.9 2,294,392 + -0.7 2,294,397 + -0.2 2,294,397 + -0.1 2,294,397 + +3.4 2,294,397 + -0.9 2,294,398 - +0.1 2,294,398 - +0.9 2,294,472 + +0.2 2,294,472 + +1.8 2,294,473 - -1.6 2,294,532 + Rru_A1983 0.18 -3.0 2,294,532 + Rru_A1983 0.18 +0.0 2,294,533 - Rru_A1983 0.18 +1.3 2,294,533 - Rru_A1983 0.18 -0.1 2,294,533 - Rru_A1983 0.18 -0.2 2,294,667 + Rru_A1983 0.83 +0.4 2,294,668 - Rru_A1983 0.84 -0.5 2,294,668 - Rru_A1983 0.84 -1.7 2,294,668 - Rru_A1983 0.84 -3.4 2,294,668 - Rru_A1983 0.84 +0.3 2,294,727 + -0.9 2,294,727 + +0.4 2,294,727 + +1.5 2,294,728 - +1.7 2,294,728 - -1.7 2,294,728 - -1.2 2,295,090 + Rru_A1984 0.49 -0.5 2,295,090 + Rru_A1984 0.49 -0.5 2,295,091 - Rru_A1984 0.49 +0.9 2,295,091 - Rru_A1984 0.49 +0.2 2,295,372 - Rru_A1985 0.24 -0.0 2,295,372 - Rru_A1985 0.24 -1.0 2,295,413 + Rru_A1985 0.33 -0.5 2,295,413 + Rru_A1985 0.33 -1.7 2,295,414 - Rru_A1985 0.34 -0.2 2,295,414 - Rru_A1985 0.34 -2.0 2,295,414 - Rru_A1985 0.34 -1.2 2,295,415 + Rru_A1985 0.34 -2.8 2,295,415 + Rru_A1985 0.34 -0.5 2,295,416 - Rru_A1985 0.34 -2.3 2,295,416 - Rru_A1985 0.34 -0.3 2,295,416 - Rru_A1985 0.34 -0.4 2,295,781 - +0.1 2,295,807 - -0.2 2,295,832 + -0.1 2,295,832 + -3.9 2,295,832 + -0.5 2,295,832 + -0.5 2,295,832 + +0.0 2,295,833 - -0.2 2,295,833 - +0.2 2,295,833 - +1.0 2,295,952 + Rru_A1986 0.17 -0.0 2,295,952 + Rru_A1986 0.17 +0.2 2,296,018 - Rru_A1986 0.26 +0.0 2,296,018 - Rru_A1986 0.26 +2.9 2,296,018 - Rru_A1986 0.26 +0.5 2,296,018 - Rru_A1986 0.26 -0.4 2,296,021 + Rru_A1986 0.26 -0.4 2,296,021 + Rru_A1986 0.26 -0.6 2,296,047 - Rru_A1986 0.30 +0.4 2,296,047 - Rru_A1986 0.30 +0.8 2,296,047 - Rru_A1986 0.30 +0.2 2,296,047 - Rru_A1986 0.30 +1.8 2,296,047 - Rru_A1986 0.30 +0.2 2,296,108 + Rru_A1986 0.38 -1.4 2,296,109 - Rru_A1986 0.39 -1.0 2,296,190 + Rru_A1986 0.50 -0.0 2,296,191 - Rru_A1986 0.50 +1.4 2,296,191 - Rru_A1986 0.50 +0.8 2,296,211 - Rru_A1986 0.53 -3.8 2,296,211 - Rru_A1986 0.53 -0.3
Or see this region's nucleotide sequence