Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1319 and Rru_A1320 are separated by 191 nucleotides Rru_A1320 and Rru_A1321 are separated by 101 nucleotides Rru_A1321 and Rru_A1322 are separated by 32 nucleotides
Rru_A1319: Rru_A1319 - Triphosphoribosyl-dephospho-CoA synthase (NCBI), at 1,557,388 to 1,558,311
_A1319
Rru_A1320: Rru_A1320 - Na+-transporting methylmalonyl-CoA/oxaloacetate decarboxylase, beta subunit (NCBI), at 1,558,503 to 1,559,714
_A1320
Rru_A1321: Rru_A1321 - Biotin--acetyl-CoA-carboxylase ligase (NCBI), at 1,559,816 to 1,560,649
_A1321
Rru_A1322: Rru_A1322 - alpha subunit of malonate decarboxylase (NCBI), at 1,560,682 to 1,562,355
_A1322
Position (kb) 1558
1559
1560 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1557.689 kb on - strand, within Rru_A1319 at 1557.727 kb on - strand, within Rru_A1319 at 1557.927 kb on + strand, within Rru_A1319 at 1558.225 kb on - strand at 1558.225 kb on - strand at 1558.382 kb on + strand at 1558.393 kb on + strand at 1558.394 kb on - strand at 1558.397 kb on + strand at 1558.397 kb on + strand at 1558.614 kb on + strand at 1558.615 kb on - strand at 1558.615 kb on - strand at 1558.625 kb on + strand, within Rru_A1320 at 1558.625 kb on + strand, within Rru_A1320 at 1558.626 kb on - strand, within Rru_A1320 at 1558.626 kb on - strand, within Rru_A1320 at 1558.769 kb on + strand, within Rru_A1320 at 1558.769 kb on + strand, within Rru_A1320 at 1558.770 kb on - strand, within Rru_A1320 at 1558.800 kb on + strand, within Rru_A1320 at 1558.801 kb on - strand, within Rru_A1320 at 1558.801 kb on - strand, within Rru_A1320 at 1558.956 kb on + strand, within Rru_A1320 at 1558.957 kb on - strand, within Rru_A1320 at 1558.959 kb on + strand, within Rru_A1320 at 1558.959 kb on + strand, within Rru_A1320 at 1558.959 kb on + strand, within Rru_A1320 at 1558.959 kb on + strand, within Rru_A1320 at 1558.959 kb on + strand, within Rru_A1320 at 1558.960 kb on - strand, within Rru_A1320 at 1558.960 kb on - strand, within Rru_A1320 at 1558.960 kb on - strand, within Rru_A1320 at 1558.960 kb on - strand, within Rru_A1320 at 1558.960 kb on - strand, within Rru_A1320 at 1558.960 kb on - strand, within Rru_A1320 at 1559.085 kb on + strand, within Rru_A1320 at 1559.085 kb on + strand, within Rru_A1320 at 1559.086 kb on - strand, within Rru_A1320 at 1559.086 kb on - strand, within Rru_A1320 at 1559.086 kb on - strand, within Rru_A1320 at 1559.091 kb on + strand, within Rru_A1320 at 1559.091 kb on + strand, within Rru_A1320 at 1559.106 kb on + strand, within Rru_A1320 at 1559.107 kb on - strand, within Rru_A1320 at 1559.121 kb on + strand, within Rru_A1320 at 1559.151 kb on + strand, within Rru_A1320 at 1559.151 kb on + strand, within Rru_A1320 at 1559.151 kb on + strand, within Rru_A1320 at 1559.349 kb on + strand, within Rru_A1320 at 1559.350 kb on - strand, within Rru_A1320 at 1559.608 kb on - strand at 1559.720 kb on - strand at 1559.720 kb on - strand at 1559.804 kb on - strand at 1560.195 kb on + strand, within Rru_A1321 at 1560.296 kb on - strand, within Rru_A1321 at 1560.696 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 1,557,689 - Rru_A1319 0.33 -1.2 1,557,727 - Rru_A1319 0.37 +1.4 1,557,927 + Rru_A1319 0.58 -3.7 1,558,225 - -0.1 1,558,225 - +1.2 1,558,382 + -0.1 1,558,393 + -1.0 1,558,394 - -0.7 1,558,397 + -2.3 1,558,397 + -3.2 1,558,614 + +0.0 1,558,615 - -2.5 1,558,615 - +0.1 1,558,625 + Rru_A1320 0.10 +1.4 1,558,625 + Rru_A1320 0.10 +0.3 1,558,626 - Rru_A1320 0.10 +1.0 1,558,626 - Rru_A1320 0.10 +0.0 1,558,769 + Rru_A1320 0.22 +1.0 1,558,769 + Rru_A1320 0.22 -4.5 1,558,770 - Rru_A1320 0.22 -1.0 1,558,800 + Rru_A1320 0.25 -0.1 1,558,801 - Rru_A1320 0.25 -0.1 1,558,801 - Rru_A1320 0.25 -2.4 1,558,956 + Rru_A1320 0.37 -0.1 1,558,957 - Rru_A1320 0.37 +0.0 1,558,959 + Rru_A1320 0.38 -0.1 1,558,959 + Rru_A1320 0.38 +0.9 1,558,959 + Rru_A1320 0.38 -0.5 1,558,959 + Rru_A1320 0.38 -0.5 1,558,959 + Rru_A1320 0.38 -0.3 1,558,960 - Rru_A1320 0.38 +2.0 1,558,960 - Rru_A1320 0.38 -0.3 1,558,960 - Rru_A1320 0.38 +0.7 1,558,960 - Rru_A1320 0.38 -0.0 1,558,960 - Rru_A1320 0.38 -1.9 1,558,960 - Rru_A1320 0.38 +0.6 1,559,085 + Rru_A1320 0.48 -0.8 1,559,085 + Rru_A1320 0.48 -2.3 1,559,086 - Rru_A1320 0.48 +3.6 1,559,086 - Rru_A1320 0.48 -0.1 1,559,086 - Rru_A1320 0.48 +0.8 1,559,091 + Rru_A1320 0.49 +0.3 1,559,091 + Rru_A1320 0.49 -0.3 1,559,106 + Rru_A1320 0.50 +0.2 1,559,107 - Rru_A1320 0.50 +0.1 1,559,121 + Rru_A1320 0.51 -0.0 1,559,151 + Rru_A1320 0.53 -0.5 1,559,151 + Rru_A1320 0.53 +0.1 1,559,151 + Rru_A1320 0.53 -0.1 1,559,349 + Rru_A1320 0.70 -1.7 1,559,350 - Rru_A1320 0.70 +0.4 1,559,608 - -2.3 1,559,720 - -0.4 1,559,720 - +1.0 1,559,804 - -1.7 1,560,195 + Rru_A1321 0.45 -0.2 1,560,296 - Rru_A1321 0.58 -1.6 1,560,696 + -0.1
Or see this region's nucleotide sequence