Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0514 and Rru_A0515 are separated by 78 nucleotides Rru_A0515 and Rru_A0516 are separated by 54 nucleotides Rru_A0516 and Rru_A0517 overlap by 4 nucleotides
Rru_A0514: Rru_A0514 - Dihydroorotate dehydrogenase 2 (NCBI), at 608,283 to 609,386
_A0514
Rru_A0515: Rru_A0515 - HAD-superfamily subfamily IIA hydrolase (NCBI), at 609,465 to 610,352
_A0515
Rru_A0516: Rru_A0516 - GCN5-related N-acetyltransferase (NCBI), at 610,407 to 610,919
_A0516
Rru_A0517: Rru_A0517 - NAD(+) kinase (NCBI), at 610,916 to 611,725
_A0517
Position (kb) 609
610
611 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 608.736 kb on + strand, within Rru_A0514 at 608.775 kb on + strand, within Rru_A0514 at 608.775 kb on + strand, within Rru_A0514 at 608.946 kb on - strand, within Rru_A0514 at 608.946 kb on - strand, within Rru_A0514 at 608.946 kb on - strand, within Rru_A0514 at 609.005 kb on + strand, within Rru_A0514 at 609.005 kb on + strand, within Rru_A0514 at 609.006 kb on - strand, within Rru_A0514 at 609.006 kb on - strand, within Rru_A0514 at 609.114 kb on + strand, within Rru_A0514 at 609.114 kb on + strand, within Rru_A0514 at 609.114 kb on + strand, within Rru_A0514 at 609.180 kb on + strand, within Rru_A0514 at 609.180 kb on + strand, within Rru_A0514 at 609.180 kb on + strand, within Rru_A0514 at 609.219 kb on + strand, within Rru_A0514 at 609.219 kb on + strand, within Rru_A0514 at 609.234 kb on + strand, within Rru_A0514 at 609.234 kb on + strand, within Rru_A0514 at 609.234 kb on + strand, within Rru_A0514 at 609.344 kb on + strand at 609.449 kb on + strand at 609.450 kb on - strand at 609.450 kb on - strand at 609.525 kb on + strand at 609.525 kb on + strand at 609.526 kb on - strand at 609.526 kb on - strand at 609.711 kb on + strand, within Rru_A0515 at 609.711 kb on + strand, within Rru_A0515 at 609.712 kb on - strand, within Rru_A0515 at 609.712 kb on - strand, within Rru_A0515 at 609.712 kb on - strand, within Rru_A0515 at 609.712 kb on - strand, within Rru_A0515 at 609.930 kb on + strand, within Rru_A0515 at 610.105 kb on - strand, within Rru_A0515 at 610.175 kb on + strand, within Rru_A0515 at 610.176 kb on - strand, within Rru_A0515 at 610.181 kb on + strand, within Rru_A0515 at 610.182 kb on - strand, within Rru_A0515 at 610.350 kb on + strand at 610.587 kb on - strand, within Rru_A0516 at 610.792 kb on - strand, within Rru_A0516
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 608,736 + Rru_A0514 0.41 -4.6 608,775 + Rru_A0514 0.45 -2.4 608,775 + Rru_A0514 0.45 -3.1 608,946 - Rru_A0514 0.60 -5.7 608,946 - Rru_A0514 0.60 -2.6 608,946 - Rru_A0514 0.60 -4.7 609,005 + Rru_A0514 0.65 +1.9 609,005 + Rru_A0514 0.65 -3.2 609,006 - Rru_A0514 0.65 -1.5 609,006 - Rru_A0514 0.65 -4.2 609,114 + Rru_A0514 0.75 -2.9 609,114 + Rru_A0514 0.75 -4.4 609,114 + Rru_A0514 0.75 -2.9 609,180 + Rru_A0514 0.81 -2.6 609,180 + Rru_A0514 0.81 -2.0 609,180 + Rru_A0514 0.81 -1.6 609,219 + Rru_A0514 0.85 -3.0 609,219 + Rru_A0514 0.85 -2.7 609,234 + Rru_A0514 0.86 -2.9 609,234 + Rru_A0514 0.86 -3.0 609,234 + Rru_A0514 0.86 -2.7 609,344 + -0.4 609,449 + +0.7 609,450 - +1.0 609,450 - -0.7 609,525 + -0.5 609,525 + +1.5 609,526 - -1.3 609,526 - +0.3 609,711 + Rru_A0515 0.28 -1.1 609,711 + Rru_A0515 0.28 -1.7 609,712 - Rru_A0515 0.28 -2.3 609,712 - Rru_A0515 0.28 -2.8 609,712 - Rru_A0515 0.28 -1.1 609,712 - Rru_A0515 0.28 +0.1 609,930 + Rru_A0515 0.52 -2.4 610,105 - Rru_A0515 0.72 -1.9 610,175 + Rru_A0515 0.80 +0.6 610,176 - Rru_A0515 0.80 -2.4 610,181 + Rru_A0515 0.81 +0.7 610,182 - Rru_A0515 0.81 -0.9 610,350 + +0.8 610,587 - Rru_A0516 0.35 -0.5 610,792 - Rru_A0516 0.75 -0.1
Or see this region's nucleotide sequence