Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0190 and Rru_A0191 are separated by 367 nucleotides Rru_A0191 and Rru_A0192 are separated by 97 nucleotides Rru_A0192 and Rru_A0193 are separated by 658 nucleotides
Rru_A0190: Rru_A0190 - TonB-dependent receptor (NCBI), at 230,821 to 232,953
_A0190
Rru_A0191: Rru_A0191 - Aspartate 1-decarboxylase-like (NCBI), at 233,321 to 233,728
_A0191
Rru_A0192: Rru_A0192 - Multi antimicrobial extrusion protein MatE (NCBI), at 233,826 to 235,253
_A0192
Rru_A0193: Rru_A0193 - Beta-ketoacyl synthase (NCBI), at 235,912 to 237,135
_A0193
Position (kb) 233
234
235
236 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 233.022 kb on + strand at 233.069 kb on + strand at 233.070 kb on - strand at 233.081 kb on + strand at 233.081 kb on + strand at 233.081 kb on + strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.115 kb on - strand at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.635 kb on + strand, within Rru_A0191 at 233.635 kb on + strand, within Rru_A0191 at 233.635 kb on + strand, within Rru_A0191 at 233.636 kb on - strand, within Rru_A0191 at 233.636 kb on - strand, within Rru_A0191 at 233.681 kb on - strand, within Rru_A0191 at 233.681 kb on - strand, within Rru_A0191 at 233.681 kb on - strand, within Rru_A0191 at 233.750 kb on + strand at 233.780 kb on - strand at 233.957 kb on + strand at 233.957 kb on + strand at 233.958 kb on - strand at 234.051 kb on + strand, within Rru_A0192 at 234.052 kb on - strand, within Rru_A0192 at 234.297 kb on - strand, within Rru_A0192 at 234.297 kb on - strand, within Rru_A0192 at 234.456 kb on + strand, within Rru_A0192 at 234.456 kb on + strand, within Rru_A0192 at 234.457 kb on - strand, within Rru_A0192 at 234.616 kb on - strand, within Rru_A0192 at 234.695 kb on + strand, within Rru_A0192 at 234.696 kb on - strand, within Rru_A0192 at 234.696 kb on - strand, within Rru_A0192 at 234.696 kb on - strand, within Rru_A0192 at 234.731 kb on + strand, within Rru_A0192 at 234.732 kb on - strand, within Rru_A0192 at 234.732 kb on - strand, within Rru_A0192 at 234.888 kb on + strand, within Rru_A0192 at 234.888 kb on + strand, within Rru_A0192 at 234.889 kb on - strand, within Rru_A0192 at 234.889 kb on - strand, within Rru_A0192 at 235.068 kb on + strand, within Rru_A0192 at 235.069 kb on - strand, within Rru_A0192 at 235.125 kb on + strand at 235.190 kb on - strand at 235.190 kb on - strand at 235.190 kb on - strand at 235.190 kb on - strand at 235.190 kb on - strand at 235.252 kb on - strand at 235.525 kb on + strand at 235.525 kb on + strand at 235.525 kb on + strand at 235.821 kb on + strand at 235.822 kb on - strand at 235.837 kb on + strand at 235.838 kb on - strand at 235.838 kb on - strand at 235.838 kb on - strand at 235.838 kb on - strand at 235.963 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 233,022 + -0.1 233,069 + -0.3 233,070 - -1.7 233,081 + -0.5 233,081 + +0.1 233,081 + -0.7 233,082 - -0.1 233,082 - -0.8 233,082 - +0.1 233,082 - -2.0 233,082 - +2.5 233,082 - +0.5 233,082 - -0.7 233,082 - -0.3 233,115 - +0.8 233,517 + Rru_A0191 0.48 -0.5 233,517 + Rru_A0191 0.48 +0.0 233,517 + Rru_A0191 0.48 -0.0 233,517 + Rru_A0191 0.48 -0.1 233,517 + Rru_A0191 0.48 +0.3 233,517 + Rru_A0191 0.48 +0.8 233,517 + Rru_A0191 0.48 +0.1 233,518 - Rru_A0191 0.48 -1.5 233,518 - Rru_A0191 0.48 -0.3 233,518 - Rru_A0191 0.48 -1.4 233,518 - Rru_A0191 0.48 +0.1 233,518 - Rru_A0191 0.48 -0.5 233,518 - Rru_A0191 0.48 +0.4 233,518 - Rru_A0191 0.48 +0.2 233,518 - Rru_A0191 0.48 -1.7 233,635 + Rru_A0191 0.77 -2.4 233,635 + Rru_A0191 0.77 -0.1 233,635 + Rru_A0191 0.77 -0.7 233,636 - Rru_A0191 0.77 +0.6 233,636 - Rru_A0191 0.77 +1.1 233,681 - Rru_A0191 0.88 -1.1 233,681 - Rru_A0191 0.88 -0.2 233,681 - Rru_A0191 0.88 -1.0 233,750 + -3.8 233,780 - +0.9 233,957 + -3.4 233,957 + -1.5 233,958 - +1.3 234,051 + Rru_A0192 0.16 -0.2 234,052 - Rru_A0192 0.16 +1.0 234,297 - Rru_A0192 0.33 -1.3 234,297 - Rru_A0192 0.33 -0.5 234,456 + Rru_A0192 0.44 +0.2 234,456 + Rru_A0192 0.44 -0.3 234,457 - Rru_A0192 0.44 -1.9 234,616 - Rru_A0192 0.55 +0.4 234,695 + Rru_A0192 0.61 +0.1 234,696 - Rru_A0192 0.61 -2.2 234,696 - Rru_A0192 0.61 +0.7 234,696 - Rru_A0192 0.61 -0.5 234,731 + Rru_A0192 0.63 -3.5 234,732 - Rru_A0192 0.63 -1.1 234,732 - Rru_A0192 0.63 -0.4 234,888 + Rru_A0192 0.74 -0.5 234,888 + Rru_A0192 0.74 +0.4 234,889 - Rru_A0192 0.74 +0.4 234,889 - Rru_A0192 0.74 -1.3 235,068 + Rru_A0192 0.87 +1.2 235,069 - Rru_A0192 0.87 -0.5 235,125 + -0.3 235,190 - -0.9 235,190 - -0.7 235,190 - -0.4 235,190 - -0.3 235,190 - +0.4 235,252 - -0.9 235,525 + +0.5 235,525 + -0.7 235,525 + +0.9 235,821 + +0.9 235,822 - +0.1 235,837 + -0.2 235,838 - -0.8 235,838 - -2.3 235,838 - -0.3 235,838 - -3.1 235,963 - +0.1
Or see this region's nucleotide sequence