Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0184 and Rru_A0185 overlap by 4 nucleotides Rru_A0185 and Rru_A0186 overlap by 4 nucleotides
Rru_A0184: Rru_A0184 - Protein of unknown function DUF324 (NCBI), at 220,433 to 222,079
_A0184
Rru_A0185: Rru_A0185 - hypothetical protein (NCBI), at 222,076 to 224,034
_A0185
Rru_A0186: Rru_A0186 - hypothetical protein (NCBI), at 224,031 to 225,704
_A0186
Position (kb) 222
223
224
225 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 221.231 kb on + strand, within Rru_A0184 at 221.231 kb on + strand, within Rru_A0184 at 221.232 kb on - strand, within Rru_A0184 at 221.607 kb on + strand, within Rru_A0184 at 221.607 kb on + strand, within Rru_A0184 at 221.607 kb on + strand, within Rru_A0184 at 221.608 kb on - strand, within Rru_A0184 at 221.841 kb on + strand, within Rru_A0184 at 221.841 kb on + strand, within Rru_A0184 at 221.842 kb on - strand, within Rru_A0184 at 222.104 kb on - strand at 222.104 kb on - strand at 222.104 kb on - strand at 222.272 kb on - strand, within Rru_A0185 at 222.299 kb on + strand, within Rru_A0185 at 222.398 kb on + strand, within Rru_A0185 at 222.398 kb on + strand, within Rru_A0185 at 222.398 kb on + strand, within Rru_A0185 at 222.399 kb on - strand, within Rru_A0185 at 222.399 kb on - strand, within Rru_A0185 at 222.402 kb on + strand, within Rru_A0185 at 222.402 kb on + strand, within Rru_A0185 at 222.402 kb on + strand, within Rru_A0185 at 222.402 kb on + strand, within Rru_A0185 at 222.402 kb on + strand, within Rru_A0185 at 222.402 kb on + strand, within Rru_A0185 at 222.402 kb on + strand, within Rru_A0185 at 222.402 kb on + strand, within Rru_A0185 at 222.652 kb on + strand, within Rru_A0185 at 222.906 kb on + strand, within Rru_A0185 at 223.276 kb on + strand, within Rru_A0185 at 223.276 kb on + strand, within Rru_A0185 at 223.277 kb on - strand, within Rru_A0185 at 223.357 kb on + strand, within Rru_A0185 at 224.071 kb on + strand at 224.072 kb on - strand at 224.211 kb on + strand, within Rru_A0186 at 224.212 kb on - strand, within Rru_A0186 at 224.253 kb on - strand, within Rru_A0186 at 224.253 kb on - strand, within Rru_A0186 at 224.330 kb on + strand, within Rru_A0186 at 224.330 kb on + strand, within Rru_A0186 at 224.330 kb on + strand, within Rru_A0186 at 224.331 kb on - strand, within Rru_A0186 at 224.331 kb on - strand, within Rru_A0186 at 224.331 kb on - strand, within Rru_A0186 at 224.331 kb on - strand, within Rru_A0186 at 224.331 kb on - strand, within Rru_A0186 at 224.465 kb on - strand, within Rru_A0186 at 224.478 kb on + strand, within Rru_A0186 at 224.478 kb on + strand, within Rru_A0186 at 224.479 kb on - strand, within Rru_A0186 at 224.479 kb on - strand, within Rru_A0186 at 224.571 kb on + strand, within Rru_A0186 at 224.571 kb on + strand, within Rru_A0186 at 224.857 kb on - strand, within Rru_A0186 at 224.946 kb on - strand, within Rru_A0186 at 224.980 kb on + strand, within Rru_A0186 at 225.032 kb on + strand, within Rru_A0186
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 221,231 + Rru_A0184 0.48 +0.6 221,231 + Rru_A0184 0.48 +0.1 221,232 - Rru_A0184 0.49 +0.0 221,607 + Rru_A0184 0.71 -1.3 221,607 + Rru_A0184 0.71 +1.9 221,607 + Rru_A0184 0.71 -0.1 221,608 - Rru_A0184 0.71 -0.2 221,841 + Rru_A0184 0.85 +0.8 221,841 + Rru_A0184 0.85 +0.4 221,842 - Rru_A0184 0.86 -4.3 222,104 - -0.9 222,104 - +0.2 222,104 - -3.6 222,272 - Rru_A0185 0.10 -1.4 222,299 + Rru_A0185 0.11 +0.8 222,398 + Rru_A0185 0.16 -0.7 222,398 + Rru_A0185 0.16 +0.0 222,398 + Rru_A0185 0.16 +0.3 222,399 - Rru_A0185 0.16 -0.6 222,399 - Rru_A0185 0.16 -0.0 222,402 + Rru_A0185 0.17 +0.3 222,402 + Rru_A0185 0.17 -2.5 222,402 + Rru_A0185 0.17 +0.1 222,402 + Rru_A0185 0.17 +0.1 222,402 + Rru_A0185 0.17 +0.7 222,402 + Rru_A0185 0.17 +0.1 222,402 + Rru_A0185 0.17 +0.1 222,402 + Rru_A0185 0.17 +0.8 222,652 + Rru_A0185 0.29 +1.1 222,906 + Rru_A0185 0.42 -0.0 223,276 + Rru_A0185 0.61 -0.5 223,276 + Rru_A0185 0.61 +1.8 223,277 - Rru_A0185 0.61 +0.4 223,357 + Rru_A0185 0.65 +0.1 224,071 + +0.1 224,072 - +0.0 224,211 + Rru_A0186 0.11 -0.6 224,212 - Rru_A0186 0.11 -0.2 224,253 - Rru_A0186 0.13 -0.4 224,253 - Rru_A0186 0.13 +1.1 224,330 + Rru_A0186 0.18 -0.3 224,330 + Rru_A0186 0.18 -0.3 224,330 + Rru_A0186 0.18 -3.2 224,331 - Rru_A0186 0.18 -2.7 224,331 - Rru_A0186 0.18 -3.7 224,331 - Rru_A0186 0.18 +2.3 224,331 - Rru_A0186 0.18 +0.9 224,331 - Rru_A0186 0.18 -1.1 224,465 - Rru_A0186 0.26 -3.1 224,478 + Rru_A0186 0.27 +0.3 224,478 + Rru_A0186 0.27 -0.4 224,479 - Rru_A0186 0.27 -4.1 224,479 - Rru_A0186 0.27 +0.2 224,571 + Rru_A0186 0.32 +0.3 224,571 + Rru_A0186 0.32 +1.2 224,857 - Rru_A0186 0.49 -0.8 224,946 - Rru_A0186 0.55 +0.7 224,980 + Rru_A0186 0.57 -0.7 225,032 + Rru_A0186 0.60 -2.2
Or see this region's nucleotide sequence