Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0110 and Rru_A0111 overlap by 4 nucleotides Rru_A0111 and Rru_A0112 overlap by 4 nucleotides Rru_A0112 and Rru_A0113 are separated by 85 nucleotides
Rru_A0110: Rru_A0110 - hypothetical protein (NCBI), at 129,366 to 130,115
_A0110
Rru_A0111: Rru_A0111 - hypothetical protein (NCBI), at 130,112 to 130,702
_A0111
Rru_A0112: Rru_A0112 - hypothetical protein (NCBI), at 130,699 to 131,421
_A0112
Rru_A0113: Rru_A0113 - type II secretion system protein E (NCBI), at 131,507 to 133,573
_A0113
Position (kb) 130
131
132 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 129.855 kb on + strand, within Rru_A0110 at 129.856 kb on - strand, within Rru_A0110 at 129.856 kb on - strand, within Rru_A0110 at 129.856 kb on - strand, within Rru_A0110 at 131.211 kb on + strand, within Rru_A0112 at 131.211 kb on + strand, within Rru_A0112 at 131.212 kb on - strand, within Rru_A0112 at 131.276 kb on - strand, within Rru_A0112 at 131.302 kb on + strand, within Rru_A0112 at 131.302 kb on + strand, within Rru_A0112 at 131.303 kb on - strand, within Rru_A0112 at 131.303 kb on - strand, within Rru_A0112 at 131.303 kb on - strand, within Rru_A0112 at 131.304 kb on + strand, within Rru_A0112 at 131.305 kb on - strand, within Rru_A0112 at 131.305 kb on - strand, within Rru_A0112 at 131.305 kb on - strand, within Rru_A0112 at 131.372 kb on - strand at 132.240 kb on - strand, within Rru_A0113 at 132.311 kb on + strand, within Rru_A0113 at 132.311 kb on + strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.398 kb on - strand, within Rru_A0113 at 132.398 kb on - strand, within Rru_A0113
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 129,855 + Rru_A0110 0.65 +0.5 129,856 - Rru_A0110 0.65 +0.0 129,856 - Rru_A0110 0.65 +0.8 129,856 - Rru_A0110 0.65 +1.8 131,211 + Rru_A0112 0.71 -0.7 131,211 + Rru_A0112 0.71 -1.1 131,212 - Rru_A0112 0.71 -0.4 131,276 - Rru_A0112 0.80 -0.5 131,302 + Rru_A0112 0.83 -1.4 131,302 + Rru_A0112 0.83 -3.7 131,303 - Rru_A0112 0.84 -0.3 131,303 - Rru_A0112 0.84 -0.1 131,303 - Rru_A0112 0.84 +0.5 131,304 + Rru_A0112 0.84 +0.6 131,305 - Rru_A0112 0.84 +0.1 131,305 - Rru_A0112 0.84 -0.2 131,305 - Rru_A0112 0.84 -0.2 131,372 - -3.9 132,240 - Rru_A0113 0.35 +0.3 132,311 + Rru_A0113 0.39 -1.2 132,311 + Rru_A0113 0.39 +1.0 132,312 - Rru_A0113 0.39 +0.3 132,312 - Rru_A0113 0.39 -0.1 132,312 - Rru_A0113 0.39 -1.2 132,312 - Rru_A0113 0.39 -0.1 132,312 - Rru_A0113 0.39 -1.6 132,312 - Rru_A0113 0.39 +0.5 132,398 - Rru_A0113 0.43 -2.2 132,398 - Rru_A0113 0.43 +0.3
Or see this region's nucleotide sequence