Experiment: sodium fluoride 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt crtL and surE are separated by 70 nucleotides surE and pheS are separated by 135 nucleotides pheS and Synpcc7942_2065 are separated by 123 nucleotides Synpcc7942_2065 and Synpcc7942_2066 are separated by 96 nucleotides
Synpcc7942_2062: crtL - lycopene cyclase (CrtL-type), at 2,138,585 to 2,139,820
crtL
Synpcc7942_2063: surE - stationary phase survival protein SurE, at 2,139,891 to 2,140,667
surE
Synpcc7942_2064: pheS - phenylalanyl-tRNA synthetase subunit alpha, at 2,140,803 to 2,141,804
pheS
Synpcc7942_2065: Synpcc7942_2065 - hypothetical protein, at 2,141,928 to 2,142,182
_2065
Synpcc7942_2066: Synpcc7942_2066 - tRNA-dihydrouridine synthase A, at 2,142,279 to 2,143,310
_2066
Position (kb)
2140
2141
2142 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2139.832 kb on + strand at 2139.832 kb on + strand at 2139.832 kb on - strand at 2139.856 kb on - strand at 2139.856 kb on - strand at 2139.865 kb on + strand at 2139.914 kb on + strand at 2139.922 kb on - strand at 2139.924 kb on + strand at 2139.932 kb on - strand at 2139.932 kb on - strand at 2140.041 kb on - strand, within surE at 2140.052 kb on + strand, within surE at 2140.130 kb on + strand, within surE at 2140.130 kb on + strand, within surE at 2140.138 kb on - strand, within surE at 2140.179 kb on + strand, within surE at 2140.179 kb on + strand, within surE at 2140.203 kb on - strand, within surE at 2140.246 kb on + strand, within surE at 2140.270 kb on - strand, within surE at 2140.278 kb on + strand, within surE at 2140.278 kb on + strand, within surE at 2140.278 kb on + strand, within surE at 2140.278 kb on + strand, within surE at 2140.285 kb on - strand, within surE at 2140.286 kb on - strand, within surE at 2140.286 kb on - strand, within surE at 2140.286 kb on - strand, within surE at 2140.291 kb on - strand, within surE at 2140.314 kb on + strand, within surE at 2140.332 kb on - strand, within surE at 2140.341 kb on - strand, within surE at 2140.341 kb on - strand, within surE at 2140.349 kb on + strand, within surE at 2140.352 kb on - strand, within surE at 2140.369 kb on - strand, within surE at 2140.403 kb on + strand, within surE at 2140.411 kb on - strand, within surE at 2140.440 kb on + strand, within surE at 2140.440 kb on + strand, within surE at 2140.440 kb on + strand, within surE at 2140.440 kb on + strand, within surE at 2140.440 kb on + strand, within surE at 2140.440 kb on + strand, within surE at 2140.448 kb on - strand, within surE at 2140.448 kb on - strand, within surE at 2140.448 kb on - strand, within surE at 2140.451 kb on + strand, within surE at 2140.480 kb on + strand, within surE at 2140.493 kb on - strand, within surE at 2140.500 kb on - strand, within surE at 2140.524 kb on - strand, within surE at 2140.526 kb on + strand, within surE at 2140.527 kb on + strand, within surE at 2140.573 kb on + strand, within surE at 2140.581 kb on + strand, within surE at 2140.595 kb on + strand at 2140.605 kb on + strand at 2140.614 kb on + strand at 2140.720 kb on - strand at 2140.722 kb on + strand at 2140.722 kb on + strand at 2140.722 kb on + strand at 2140.722 kb on + strand at 2140.730 kb on - strand at 2141.959 kb on + strand, within Synpcc7942_2065 at 2142.041 kb on - strand, within Synpcc7942_2065 at 2142.200 kb on + strand at 2142.239 kb on - strand at 2142.255 kb on - strand at 2142.255 kb on - strand at 2142.294 kb on + strand at 2142.300 kb on - strand at 2142.302 kb on - strand at 2142.368 kb on - strand at 2142.376 kb on + strand at 2142.376 kb on + strand at 2142.376 kb on + strand at 2142.384 kb on - strand, within Synpcc7942_2066 at 2142.412 kb on + strand, within Synpcc7942_2066 at 2142.429 kb on - strand, within Synpcc7942_2066 at 2142.430 kb on - strand, within Synpcc7942_2066 at 2142.460 kb on + strand, within Synpcc7942_2066 at 2142.466 kb on - strand, within Synpcc7942_2066 at 2142.476 kb on + strand, within Synpcc7942_2066 at 2142.484 kb on - strand, within Synpcc7942_2066 at 2142.524 kb on + strand, within Synpcc7942_2066 at 2142.530 kb on + strand, within Synpcc7942_2066 at 2142.532 kb on - strand, within Synpcc7942_2066 at 2142.538 kb on + strand, within Synpcc7942_2066 at 2142.538 kb on + strand, within Synpcc7942_2066 at 2142.538 kb on + strand, within Synpcc7942_2066 at 2142.607 kb on + strand, within Synpcc7942_2066 at 2142.625 kb on - strand, within Synpcc7942_2066 at 2142.634 kb on + strand, within Synpcc7942_2066 at 2142.642 kb on - strand, within Synpcc7942_2066 at 2142.750 kb on + strand, within Synpcc7942_2066 at 2142.770 kb on - strand, within Synpcc7942_2066 at 2142.785 kb on - strand, within Synpcc7942_2066 at 2142.794 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 25 mM remove 2,139,832 + +0.9 2,139,832 + -1.7 2,139,832 - -0.7 2,139,856 - +1.6 2,139,856 - +2.1 2,139,865 + +2.9 2,139,914 + -1.7 2,139,922 - +1.9 2,139,924 + -0.7 2,139,932 - -2.3 2,139,932 - -1.7 2,140,041 - surE Synpcc7942_2063 0.19 -3.3 2,140,052 + surE Synpcc7942_2063 0.21 -1.7 2,140,130 + surE Synpcc7942_2063 0.31 -2.3 2,140,130 + surE Synpcc7942_2063 0.31 -3.1 2,140,138 - surE Synpcc7942_2063 0.32 -2.7 2,140,179 + surE Synpcc7942_2063 0.37 -2.7 2,140,179 + surE Synpcc7942_2063 0.37 -2.3 2,140,203 - surE Synpcc7942_2063 0.40 -3.9 2,140,246 + surE Synpcc7942_2063 0.46 -2.3 2,140,270 - surE Synpcc7942_2063 0.49 -1.7 2,140,278 + surE Synpcc7942_2063 0.50 -3.5 2,140,278 + surE Synpcc7942_2063 0.50 -1.7 2,140,278 + surE Synpcc7942_2063 0.50 -2.7 2,140,278 + surE Synpcc7942_2063 0.50 -1.7 2,140,285 - surE Synpcc7942_2063 0.51 -0.7 2,140,286 - surE Synpcc7942_2063 0.51 -3.1 2,140,286 - surE Synpcc7942_2063 0.51 -2.7 2,140,286 - surE Synpcc7942_2063 0.51 -3.7 2,140,291 - surE Synpcc7942_2063 0.51 -1.1 2,140,314 + surE Synpcc7942_2063 0.54 -0.7 2,140,332 - surE Synpcc7942_2063 0.57 -2.7 2,140,341 - surE Synpcc7942_2063 0.58 -2.3 2,140,341 - surE Synpcc7942_2063 0.58 -1.3 2,140,349 + surE Synpcc7942_2063 0.59 -3.5 2,140,352 - surE Synpcc7942_2063 0.59 -2.7 2,140,369 - surE Synpcc7942_2063 0.62 -2.3 2,140,403 + surE Synpcc7942_2063 0.66 -0.7 2,140,411 - surE Synpcc7942_2063 0.67 -2.3 2,140,440 + surE Synpcc7942_2063 0.71 +1.5 2,140,440 + surE Synpcc7942_2063 0.71 -1.7 2,140,440 + surE Synpcc7942_2063 0.71 -0.7 2,140,440 + surE Synpcc7942_2063 0.71 -2.7 2,140,440 + surE Synpcc7942_2063 0.71 -2.3 2,140,440 + surE Synpcc7942_2063 0.71 -3.5 2,140,448 - surE Synpcc7942_2063 0.72 +0.3 2,140,448 - surE Synpcc7942_2063 0.72 -4.2 2,140,448 - surE Synpcc7942_2063 0.72 -2.7 2,140,451 + surE Synpcc7942_2063 0.72 -1.7 2,140,480 + surE Synpcc7942_2063 0.76 -0.7 2,140,493 - surE Synpcc7942_2063 0.77 -2.3 2,140,500 - surE Synpcc7942_2063 0.78 -1.7 2,140,524 - surE Synpcc7942_2063 0.81 -3.3 2,140,526 + surE Synpcc7942_2063 0.82 -3.1 2,140,527 + surE Synpcc7942_2063 0.82 -2.3 2,140,573 + surE Synpcc7942_2063 0.88 -0.7 2,140,581 + surE Synpcc7942_2063 0.89 -0.7 2,140,595 + -3.7 2,140,605 + -2.1 2,140,614 + -1.7 2,140,720 - +1.1 2,140,722 + +1.3 2,140,722 + +1.0 2,140,722 + +3.0 2,140,722 + +3.8 2,140,730 - +1.9 2,141,959 + Synpcc7942_2065 0.12 -1.7 2,142,041 - Synpcc7942_2065 0.44 -2.3 2,142,200 + -0.7 2,142,239 - +0.7 2,142,255 - +0.3 2,142,255 - -1.7 2,142,294 + -0.7 2,142,300 - +0.6 2,142,302 - +2.1 2,142,368 - -3.0 2,142,376 + +1.0 2,142,376 + +1.6 2,142,376 + -2.0 2,142,384 - Synpcc7942_2066 0.10 -0.7 2,142,412 + Synpcc7942_2066 0.13 +2.4 2,142,429 - Synpcc7942_2066 0.15 +1.7 2,142,430 - Synpcc7942_2066 0.15 +0.7 2,142,460 + Synpcc7942_2066 0.18 -2.3 2,142,466 - Synpcc7942_2066 0.18 +1.4 2,142,476 + Synpcc7942_2066 0.19 -2.3 2,142,484 - Synpcc7942_2066 0.20 +1.7 2,142,524 + Synpcc7942_2066 0.24 +1.6 2,142,530 + Synpcc7942_2066 0.24 -0.4 2,142,532 - Synpcc7942_2066 0.25 -2.3 2,142,538 + Synpcc7942_2066 0.25 +0.9 2,142,538 + Synpcc7942_2066 0.25 +0.9 2,142,538 + Synpcc7942_2066 0.25 -0.7 2,142,607 + Synpcc7942_2066 0.32 -1.7 2,142,625 - Synpcc7942_2066 0.34 +1.1 2,142,634 + Synpcc7942_2066 0.34 +2.3 2,142,642 - Synpcc7942_2066 0.35 +3.4 2,142,750 + Synpcc7942_2066 0.46 +1.9 2,142,770 - Synpcc7942_2066 0.48 -1.7 2,142,785 - Synpcc7942_2066 0.49 +0.6 2,142,794 + -1.7
Or see this region's nucleotide sequence