Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00128 and SMc00457 overlap by 8 nucleotides SMc00457 and SMc00122 are separated by 97 nucleotides SMc00122 and SMc00064 are separated by 120 nucleotides SMc00064 and SMc00123 are separated by 61 nucleotides
SMc00128: SMc00128 - hypothetical protein, at 1,099,690 to 1,100,235
SMc00128
SMc00457: SMc00457 - hypothetical protein, at 1,100,228 to 1,100,815
SMc00457
SMc00122: SMc00122 - penicillin-binding protein, at 1,100,913 to 1,103,147
SMc00122
SMc00064: SMc00064 - hypothetical protein, at 1,103,268 to 1,103,735
SMc00064
SMc00123: SMc00123 - hypothetical protein, at 1,103,797 to 1,104,132
SMc00123
Position (kb)
1100
1101
1102
1103
1104 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1100.846 kb on - strand at 1100.846 kb on - strand at 1103.154 kb on - strand at 1103.154 kb on - strand at 1103.154 kb on - strand at 1103.154 kb on - strand at 1103.455 kb on - strand, within SMc00064 at 1103.575 kb on - strand, within SMc00064 at 1103.575 kb on - strand, within SMc00064 at 1103.688 kb on + strand, within SMc00064 at 1103.689 kb on - strand at 1103.707 kb on + strand at 1103.708 kb on - strand at 1103.708 kb on - strand at 1103.768 kb on + strand at 1103.769 kb on - strand at 1103.769 kb on - strand at 1104.143 kb on + strand at 1104.143 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 1,100,846 - +0.2 1,100,846 - -0.4 1,103,154 - +0.3 1,103,154 - +0.8 1,103,154 - -0.2 1,103,154 - +0.8 1,103,455 - SMc00064 0.40 -0.5 1,103,575 - SMc00064 0.66 -3.7 1,103,575 - SMc00064 0.66 -0.1 1,103,688 + SMc00064 0.90 +0.5 1,103,689 - +0.8 1,103,707 + -0.5 1,103,708 - -1.4 1,103,708 - +3.1 1,103,768 + +0.9 1,103,769 - -0.8 1,103,769 - -1.4 1,104,143 + +0.7 1,104,143 + +1.6
Or see this region's nucleotide sequence