Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0045 and SMa5015 are separated by 308 nucleotides SMa5015 and SMa0050 are separated by 140 nucleotides SMa0050 and SMa0052 overlap by 10 nucleotides SMa0052 and SMa0056 are separated by 581 nucleotides
SMa0045: SMa0045 - carbonic anhydrase, at 25,848 to 26,597
SMa0045
SMa5015: SMa5015 - hypothetical protein, at 26,906 to 27,154
SMa5015
SMa0050: SMa0050 - hypothetical protein, at 27,295 to 27,516
SMa0050
SMa0052: SMa0052 - hypothetical protein, at 27,507 to 27,713
SMa0052
SMa0056: SMa0056 - dehydratase/enolase, at 28,295 to 29,428
SMa0056
Position (kb) 27
28 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 26.601 kb on + strand at 26.657 kb on + strand at 26.657 kb on + strand at 26.657 kb on + strand at 26.658 kb on - strand at 26.658 kb on - strand at 26.658 kb on - strand at 26.705 kb on + strand at 26.706 kb on - strand at 26.730 kb on + strand at 26.902 kb on + strand at 26.966 kb on - strand, within SMa5015 at 27.119 kb on + strand, within SMa5015 at 27.251 kb on - strand at 27.310 kb on + strand at 27.435 kb on + strand, within SMa0050 at 27.435 kb on + strand, within SMa0050 at 27.435 kb on + strand, within SMa0050 at 27.436 kb on - strand, within SMa0050 at 27.599 kb on + strand, within SMa0052 at 27.599 kb on + strand, within SMa0052 at 27.600 kb on - strand, within SMa0052 at 27.621 kb on + strand, within SMa0052 at 27.621 kb on + strand, within SMa0052 at 27.645 kb on - strand, within SMa0052 at 27.782 kb on + strand at 27.783 kb on - strand at 27.797 kb on + strand at 27.844 kb on - strand at 27.888 kb on - strand at 27.966 kb on + strand at 27.966 kb on + strand at 27.966 kb on + strand at 27.966 kb on + strand at 28.027 kb on + strand at 28.027 kb on + strand at 28.028 kb on - strand at 28.028 kb on - strand at 28.274 kb on + strand at 28.274 kb on + strand at 28.296 kb on + strand at 28.296 kb on + strand at 28.297 kb on - strand at 28.371 kb on + strand at 28.371 kb on + strand at 28.371 kb on + strand at 28.410 kb on + strand, within SMa0056 at 28.430 kb on + strand, within SMa0056 at 28.633 kb on + strand, within SMa0056
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 26,601 + -1.5 26,657 + -4.6 26,657 + -0.5 26,657 + +0.5 26,658 - +0.7 26,658 - +0.8 26,658 - -2.0 26,705 + +0.0 26,706 - +0.4 26,730 + +2.9 26,902 + +0.9 26,966 - SMa5015 0.24 -1.6 27,119 + SMa5015 0.86 -0.3 27,251 - -1.5 27,310 + -1.6 27,435 + SMa0050 0.63 +4.1 27,435 + SMa0050 0.63 +0.3 27,435 + SMa0050 0.63 +0.7 27,436 - SMa0050 0.64 +0.9 27,599 + SMa0052 0.44 -3.3 27,599 + SMa0052 0.44 -0.2 27,600 - SMa0052 0.45 -0.2 27,621 + SMa0052 0.55 +2.2 27,621 + SMa0052 0.55 -3.3 27,645 - SMa0052 0.67 +2.5 27,782 + +0.7 27,783 - -2.3 27,797 + +0.4 27,844 - +0.4 27,888 - +3.3 27,966 + -2.5 27,966 + +2.3 27,966 + -1.9 27,966 + -0.7 28,027 + +0.6 28,027 + -0.2 28,028 - +0.1 28,028 - +0.2 28,274 + +0.9 28,274 + -1.9 28,296 + -3.3 28,296 + +1.1 28,297 - +1.0 28,371 + +1.4 28,371 + -0.1 28,371 + +1.7 28,410 + SMa0056 0.10 +2.3 28,430 + SMa0056 0.12 +0.9 28,633 + SMa0056 0.30 -0.9
Or see this region's nucleotide sequence