Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20113 and SM_b20114 are separated by 2 nucleotides SM_b20114 and SM_b20115 are separated by 26 nucleotides SM_b20115 and SM_b20116 are separated by 134 nucleotides
SM_b20113: SM_b20113 - dehydrogenase, at 124,709 to 125,641
_b20113
SM_b20114: SM_b20114 - hypothetical protein, at 125,644 to 126,369
_b20114
SM_b20115: SM_b20115 - dihydroxy-acid dehydratase, at 126,396 to 128,117
_b20115
SM_b20116: SM_b20116 - hypothetical protein, at 128,252 to 130,456
_b20116
Position (kb) 126
127
128
129 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 125.412 kb on + strand, within SM_b20113 at 125.465 kb on - strand, within SM_b20113 at 125.558 kb on - strand at 125.646 kb on - strand at 125.709 kb on - strand at 126.197 kb on + strand, within SM_b20114 at 126.198 kb on - strand, within SM_b20114 at 126.198 kb on - strand, within SM_b20114 at 126.208 kb on - strand, within SM_b20114 at 126.319 kb on + strand at 126.319 kb on + strand at 126.359 kb on + strand at 126.359 kb on + strand at 126.359 kb on + strand at 126.359 kb on + strand at 126.360 kb on - strand at 126.360 kb on - strand at 126.360 kb on - strand at 126.503 kb on - strand at 126.515 kb on - strand at 126.558 kb on + strand at 126.688 kb on + strand, within SM_b20115 at 126.773 kb on + strand, within SM_b20115 at 127.009 kb on + strand, within SM_b20115 at 127.010 kb on - strand, within SM_b20115 at 127.010 kb on - strand, within SM_b20115 at 127.162 kb on + strand, within SM_b20115 at 127.162 kb on + strand, within SM_b20115 at 127.163 kb on - strand, within SM_b20115 at 127.180 kb on + strand, within SM_b20115 at 127.181 kb on - strand, within SM_b20115 at 127.181 kb on - strand, within SM_b20115 at 127.430 kb on + strand, within SM_b20115 at 127.430 kb on + strand, within SM_b20115 at 127.431 kb on - strand, within SM_b20115 at 127.547 kb on - strand, within SM_b20115 at 127.622 kb on - strand, within SM_b20115 at 127.770 kb on + strand, within SM_b20115 at 128.127 kb on + strand at 128.128 kb on - strand at 128.159 kb on + strand at 128.159 kb on + strand at 128.159 kb on + strand at 128.160 kb on - strand at 128.160 kb on - strand at 128.160 kb on - strand at 128.160 kb on - strand at 128.469 kb on + strand at 128.518 kb on - strand, within SM_b20116 at 128.561 kb on + strand, within SM_b20116 at 128.561 kb on + strand, within SM_b20116 at 128.561 kb on + strand, within SM_b20116 at 128.561 kb on + strand, within SM_b20116 at 128.589 kb on + strand, within SM_b20116 at 128.590 kb on - strand, within SM_b20116 at 128.646 kb on + strand, within SM_b20116 at 128.706 kb on + strand, within SM_b20116 at 128.707 kb on - strand, within SM_b20116 at 128.795 kb on + strand, within SM_b20116 at 128.796 kb on - strand, within SM_b20116 at 128.901 kb on + strand, within SM_b20116 at 128.902 kb on - strand, within SM_b20116 at 129.042 kb on + strand, within SM_b20116 at 129.042 kb on + strand, within SM_b20116 at 129.042 kb on + strand, within SM_b20116 at 129.042 kb on + strand, within SM_b20116 at 129.051 kb on - strand, within SM_b20116
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 125,412 + SM_b20113 0.75 -0.3 125,465 - SM_b20113 0.81 +0.4 125,558 - -3.0 125,646 - -2.5 125,709 - +0.2 126,197 + SM_b20114 0.76 -3.5 126,198 - SM_b20114 0.76 +1.1 126,198 - SM_b20114 0.76 +0.7 126,208 - SM_b20114 0.78 +0.0 126,319 + +0.2 126,319 + -3.6 126,359 + -0.4 126,359 + -1.3 126,359 + -0.2 126,359 + -0.2 126,360 - +0.6 126,360 - +1.8 126,360 - -2.2 126,503 - -0.7 126,515 - -0.1 126,558 + -0.3 126,688 + SM_b20115 0.17 -2.6 126,773 + SM_b20115 0.22 -1.3 127,009 + SM_b20115 0.36 -0.3 127,010 - SM_b20115 0.36 -0.7 127,010 - SM_b20115 0.36 -1.8 127,162 + SM_b20115 0.44 -1.4 127,162 + SM_b20115 0.44 -0.2 127,163 - SM_b20115 0.45 -1.7 127,180 + SM_b20115 0.46 +0.9 127,181 - SM_b20115 0.46 +0.6 127,181 - SM_b20115 0.46 +0.4 127,430 + SM_b20115 0.60 -0.7 127,430 + SM_b20115 0.60 +0.4 127,431 - SM_b20115 0.60 +1.1 127,547 - SM_b20115 0.67 -0.3 127,622 - SM_b20115 0.71 +1.4 127,770 + SM_b20115 0.80 -2.6 128,127 + -0.3 128,128 - +2.8 128,159 + -1.7 128,159 + +0.4 128,159 + -0.2 128,160 - -2.9 128,160 - -1.0 128,160 - -3.3 128,160 - +0.1 128,469 + -1.9 128,518 - SM_b20116 0.12 -0.6 128,561 + SM_b20116 0.14 +3.0 128,561 + SM_b20116 0.14 +0.0 128,561 + SM_b20116 0.14 -2.3 128,561 + SM_b20116 0.14 +0.6 128,589 + SM_b20116 0.15 +1.4 128,590 - SM_b20116 0.15 +1.8 128,646 + SM_b20116 0.18 -0.3 128,706 + SM_b20116 0.21 -1.9 128,707 - SM_b20116 0.21 -1.3 128,795 + SM_b20116 0.25 +0.1 128,796 - SM_b20116 0.25 -0.4 128,901 + SM_b20116 0.29 +0.3 128,902 - SM_b20116 0.29 -0.1 129,042 + SM_b20116 0.36 -1.6 129,042 + SM_b20116 0.36 -0.0 129,042 + SM_b20116 0.36 +0.5 129,042 + SM_b20116 0.36 +0.1 129,051 - SM_b20116 0.36 -0.9
Or see this region's nucleotide sequence