Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09335 and RR42_RS09340 are separated by 100 nucleotides RR42_RS09340 and RR42_RS09345 are separated by 141 nucleotides RR42_RS09345 and RR42_RS09350 are separated by 303 nucleotides
RR42_RS09335: RR42_RS09335 - DNA polymerase II, at 1,974,110 to 1,976,485
_RS09335
RR42_RS09340: RR42_RS09340 - lysine transporter LysE, at 1,976,586 to 1,977,218
_RS09340
RR42_RS09345: RR42_RS09345 - limonene-1,2-epoxide hydrolase, at 1,977,360 to 1,977,803
_RS09345
RR42_RS09350: RR42_RS09350 - AraC family transcriptional regulator, at 1,978,107 to 1,978,682
_RS09350
Position (kb)
1977
1978 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1976.602 kb on + strand at 1976.603 kb on - strand at 1976.603 kb on - strand at 1976.777 kb on + strand, within RR42_RS09340 at 1976.783 kb on + strand, within RR42_RS09340 at 1976.783 kb on + strand, within RR42_RS09340 at 1976.784 kb on - strand, within RR42_RS09340 at 1976.804 kb on + strand, within RR42_RS09340 at 1976.827 kb on + strand, within RR42_RS09340 at 1976.828 kb on - strand, within RR42_RS09340 at 1976.828 kb on - strand, within RR42_RS09340 at 1976.828 kb on - strand, within RR42_RS09340 at 1976.859 kb on - strand, within RR42_RS09340 at 1976.865 kb on - strand, within RR42_RS09340 at 1976.963 kb on - strand, within RR42_RS09340 at 1976.963 kb on - strand, within RR42_RS09340 at 1976.963 kb on - strand, within RR42_RS09340 at 1976.972 kb on - strand, within RR42_RS09340 at 1977.008 kb on - strand, within RR42_RS09340 at 1977.059 kb on - strand, within RR42_RS09340 at 1977.239 kb on - strand at 1977.242 kb on + strand at 1977.242 kb on + strand at 1977.242 kb on + strand at 1977.242 kb on + strand at 1977.293 kb on + strand at 1977.294 kb on - strand at 1977.329 kb on + strand at 1977.344 kb on + strand at 1977.345 kb on - strand at 1977.387 kb on - strand at 1977.418 kb on + strand, within RR42_RS09345 at 1977.418 kb on + strand, within RR42_RS09345 at 1977.419 kb on - strand, within RR42_RS09345 at 1977.419 kb on - strand, within RR42_RS09345 at 1977.419 kb on - strand, within RR42_RS09345 at 1977.419 kb on - strand, within RR42_RS09345 at 1977.419 kb on - strand, within RR42_RS09345 at 1977.520 kb on + strand, within RR42_RS09345 at 1977.679 kb on + strand, within RR42_RS09345 at 1977.680 kb on - strand, within RR42_RS09345 at 1977.680 kb on - strand, within RR42_RS09345 at 1977.680 kb on - strand, within RR42_RS09345 at 1977.684 kb on + strand, within RR42_RS09345 at 1977.684 kb on + strand, within RR42_RS09345 at 1977.685 kb on - strand, within RR42_RS09345 at 1977.685 kb on - strand, within RR42_RS09345 at 1977.798 kb on - strand at 1977.798 kb on - strand at 1977.977 kb on - strand at 1977.983 kb on - strand at 1977.992 kb on + strand at 1978.011 kb on + strand at 1978.011 kb on + strand at 1978.012 kb on - strand at 1978.040 kb on + strand at 1978.040 kb on + strand at 1978.040 kb on + strand at 1978.041 kb on - strand at 1978.041 kb on - strand at 1978.046 kb on - strand at 1978.046 kb on - strand at 1978.049 kb on - strand at 1978.095 kb on + strand at 1978.138 kb on + strand at 1978.196 kb on - strand, within RR42_RS09350 at 1978.227 kb on + strand, within RR42_RS09350 at 1978.252 kb on - strand, within RR42_RS09350 at 1978.306 kb on - strand, within RR42_RS09350 at 1978.473 kb on + strand, within RR42_RS09350 at 1978.473 kb on + strand, within RR42_RS09350 at 1978.474 kb on - strand, within RR42_RS09350 at 1978.482 kb on + strand, within RR42_RS09350 at 1978.482 kb on + strand, within RR42_RS09350 at 1978.483 kb on - strand, within RR42_RS09350 at 1978.540 kb on + strand, within RR42_RS09350 at 1978.637 kb on - strand at 1978.789 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 1,976,602 + +0.1 1,976,603 - -0.4 1,976,603 - +0.1 1,976,777 + RR42_RS09340 0.30 -0.5 1,976,783 + RR42_RS09340 0.31 +0.9 1,976,783 + RR42_RS09340 0.31 -1.3 1,976,784 - RR42_RS09340 0.31 -0.3 1,976,804 + RR42_RS09340 0.34 -0.8 1,976,827 + RR42_RS09340 0.38 -0.6 1,976,828 - RR42_RS09340 0.38 +0.2 1,976,828 - RR42_RS09340 0.38 +0.2 1,976,828 - RR42_RS09340 0.38 -0.2 1,976,859 - RR42_RS09340 0.43 +0.0 1,976,865 - RR42_RS09340 0.44 +1.5 1,976,963 - RR42_RS09340 0.60 -0.9 1,976,963 - RR42_RS09340 0.60 +0.6 1,976,963 - RR42_RS09340 0.60 +0.1 1,976,972 - RR42_RS09340 0.61 +0.0 1,977,008 - RR42_RS09340 0.67 -0.2 1,977,059 - RR42_RS09340 0.75 -0.7 1,977,239 - -0.2 1,977,242 + +0.6 1,977,242 + -0.1 1,977,242 + +0.0 1,977,242 + -0.9 1,977,293 + -0.0 1,977,294 - +0.5 1,977,329 + -0.3 1,977,344 + +0.2 1,977,345 - -1.0 1,977,387 - +0.6 1,977,418 + RR42_RS09345 0.13 -0.6 1,977,418 + RR42_RS09345 0.13 -0.4 1,977,419 - RR42_RS09345 0.13 +0.6 1,977,419 - RR42_RS09345 0.13 +0.7 1,977,419 - RR42_RS09345 0.13 -0.3 1,977,419 - RR42_RS09345 0.13 +0.5 1,977,419 - RR42_RS09345 0.13 +0.9 1,977,520 + RR42_RS09345 0.36 +0.8 1,977,679 + RR42_RS09345 0.72 +0.4 1,977,680 - RR42_RS09345 0.72 -0.6 1,977,680 - RR42_RS09345 0.72 +1.6 1,977,680 - RR42_RS09345 0.72 +1.8 1,977,684 + RR42_RS09345 0.73 +0.4 1,977,684 + RR42_RS09345 0.73 +0.6 1,977,685 - RR42_RS09345 0.73 +0.4 1,977,685 - RR42_RS09345 0.73 -0.8 1,977,798 - +0.3 1,977,798 - -0.1 1,977,977 - -0.8 1,977,983 - +1.2 1,977,992 + +1.1 1,978,011 + -0.6 1,978,011 + +0.2 1,978,012 - -0.7 1,978,040 + -0.3 1,978,040 + +0.1 1,978,040 + -0.6 1,978,041 - +0.2 1,978,041 - +0.6 1,978,046 - -1.3 1,978,046 - -0.4 1,978,049 - -0.3 1,978,095 + -1.4 1,978,138 + -0.0 1,978,196 - RR42_RS09350 0.15 -1.0 1,978,227 + RR42_RS09350 0.21 +0.1 1,978,252 - RR42_RS09350 0.25 -0.0 1,978,306 - RR42_RS09350 0.35 +0.6 1,978,473 + RR42_RS09350 0.64 -2.6 1,978,473 + RR42_RS09350 0.64 +0.2 1,978,474 - RR42_RS09350 0.64 -0.8 1,978,482 + RR42_RS09350 0.65 +0.9 1,978,482 + RR42_RS09350 0.65 -0.4 1,978,483 - RR42_RS09350 0.65 -0.3 1,978,540 + RR42_RS09350 0.75 +0.6 1,978,637 - -0.3 1,978,789 + -0.7
Or see this region's nucleotide sequence