Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00010 and RR42_RS00015 are separated by 227 nucleotides RR42_RS00015 and RR42_RS00020 are separated by 364 nucleotides
RR42_RS00010: RR42_RS00010 - DNA polymerase III subunit beta, at 2,872 to 3,996
_RS00010
RR42_RS00015: RR42_RS00015 - DNA gyrase subunit B, at 4,224 to 6,761
_RS00015
RR42_RS00020: RR42_RS00020 - hypothetical protein, at 7,126 to 7,770
_RS00020
Position (kb) 4
5
6
7 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4.041 kb on + strand at 4.041 kb on + strand at 4.144 kb on + strand at 4.215 kb on + strand at 6.838 kb on + strand at 6.863 kb on + strand at 6.931 kb on - strand at 6.952 kb on - strand at 7.032 kb on + strand at 7.033 kb on - strand at 7.037 kb on + strand at 7.037 kb on + strand at 7.093 kb on - strand at 7.098 kb on + strand at 7.099 kb on - strand at 7.099 kb on - strand at 7.151 kb on + strand at 7.304 kb on + strand, within RR42_RS00020 at 7.305 kb on - strand, within RR42_RS00020 at 7.326 kb on + strand, within RR42_RS00020 at 7.329 kb on + strand, within RR42_RS00020 at 7.352 kb on + strand, within RR42_RS00020 at 7.402 kb on - strand, within RR42_RS00020 at 7.607 kb on + strand, within RR42_RS00020 at 7.608 kb on - strand, within RR42_RS00020 at 7.608 kb on - strand, within RR42_RS00020 at 7.642 kb on - strand, within RR42_RS00020 at 7.663 kb on + strand, within RR42_RS00020
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 4,041 + -0.4 4,041 + +0.1 4,144 + +0.3 4,215 + +1.2 6,838 + -0.1 6,863 + +0.5 6,931 - +0.7 6,952 - -0.2 7,032 + +0.5 7,033 - -0.6 7,037 + +0.3 7,037 + +1.2 7,093 - +0.2 7,098 + +0.6 7,099 - +0.2 7,099 - -0.4 7,151 + -0.1 7,304 + RR42_RS00020 0.28 +0.0 7,305 - RR42_RS00020 0.28 +0.3 7,326 + RR42_RS00020 0.31 +0.9 7,329 + RR42_RS00020 0.31 +0.6 7,352 + RR42_RS00020 0.35 +0.2 7,402 - RR42_RS00020 0.43 -2.0 7,607 + RR42_RS00020 0.75 +0.8 7,608 - RR42_RS00020 0.75 +1.8 7,608 - RR42_RS00020 0.75 +1.1 7,642 - RR42_RS00020 0.80 +0.8 7,663 + RR42_RS00020 0.83 +2.2
Or see this region's nucleotide sequence