Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03120 and MMP_RS03125 overlap by 4 nucleotides MMP_RS03125 and MMP_RS03130 are separated by 76 nucleotides MMP_RS03130 and MMP_RS03135 are separated by 18 nucleotides
MMP_RS03120: MMP_RS03120 - hypothetical protein, at 588,738 to 588,920
_RS03120
MMP_RS03125: MMP_RS03125 - cell division ATPase MinD, at 588,917 to 589,702
_RS03125
MMP_RS03130: MMP_RS03130 - hypothetical protein, at 589,779 to 590,345
_RS03130
MMP_RS03135: MMP_RS03135 - O-phosphoseryl-tRNA(Sec) selenium transferase, at 590,364 to 591,674
_RS03135
Position (kb) 589
590
591 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 588.880 kb on + strand, within MMP_RS03120 at 588.880 kb on + strand, within MMP_RS03120 at 588.888 kb on - strand, within MMP_RS03120 at 588.891 kb on - strand, within MMP_RS03120 at 588.938 kb on - strand at 588.993 kb on - strand at 589.069 kb on - strand, within MMP_RS03125 at 589.129 kb on - strand, within MMP_RS03125 at 589.284 kb on + strand, within MMP_RS03125 at 589.295 kb on - strand, within MMP_RS03125 at 589.322 kb on + strand, within MMP_RS03125 at 589.596 kb on + strand, within MMP_RS03125 at 589.683 kb on + strand at 589.736 kb on - strand at 589.737 kb on + strand at 589.737 kb on + strand at 589.738 kb on - strand at 589.738 kb on - strand at 589.738 kb on - strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.739 kb on + strand at 589.740 kb on - strand at 589.741 kb on + strand at 589.746 kb on + strand at 589.754 kb on + strand at 589.764 kb on - strand at 589.767 kb on - strand at 589.767 kb on - strand at 589.767 kb on - strand at 589.767 kb on - strand at 589.768 kb on + strand at 589.769 kb on - strand at 589.769 kb on - strand at 589.780 kb on - strand at 589.827 kb on + strand at 589.827 kb on + strand at 589.887 kb on + strand, within MMP_RS03130 at 589.889 kb on - strand, within MMP_RS03130 at 589.897 kb on + strand, within MMP_RS03130 at 590.016 kb on + strand, within MMP_RS03130 at 590.031 kb on - strand, within MMP_RS03130 at 590.055 kb on - strand, within MMP_RS03130 at 590.060 kb on - strand, within MMP_RS03130 at 590.100 kb on + strand, within MMP_RS03130 at 590.100 kb on + strand, within MMP_RS03130 at 590.103 kb on + strand, within MMP_RS03130 at 590.267 kb on - strand, within MMP_RS03130 at 590.277 kb on - strand, within MMP_RS03130 at 590.323 kb on - strand at 590.387 kb on - strand at 590.401 kb on + strand at 590.446 kb on - strand at 590.528 kb on - strand at 590.730 kb on + strand at 590.905 kb on - strand at 590.983 kb on + strand at 591.114 kb on - strand at 591.321 kb on - strand at 591.340 kb on - strand at 591.341 kb on + strand at 591.341 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 588,880 + MMP_RS03120 0.78 +0.2 588,880 + MMP_RS03120 0.78 +0.9 588,888 - MMP_RS03120 0.82 -0.8 588,891 - MMP_RS03120 0.84 -0.1 588,938 - +0.8 588,993 - +1.5 589,069 - MMP_RS03125 0.19 +1.5 589,129 - MMP_RS03125 0.27 +1.0 589,284 + MMP_RS03125 0.47 +0.9 589,295 - MMP_RS03125 0.48 +1.6 589,322 + MMP_RS03125 0.52 +1.1 589,596 + MMP_RS03125 0.86 +1.0 589,683 + +1.0 589,736 - +0.1 589,737 + +1.0 589,737 + +1.1 589,738 - +0.7 589,738 - +1.4 589,738 - +0.5 589,739 + +1.6 589,739 + +1.2 589,739 + +1.6 589,739 + +1.3 589,739 + +0.8 589,740 - +0.3 589,741 + +2.1 589,746 + +1.4 589,754 + +1.1 589,764 - +0.8 589,767 - +0.9 589,767 - +1.0 589,767 - +1.3 589,767 - +0.5 589,768 + +1.1 589,769 - +0.2 589,769 - +1.1 589,780 - +0.7 589,827 + -1.1 589,827 + -1.0 589,887 + MMP_RS03130 0.19 -1.3 589,889 - MMP_RS03130 0.19 -0.6 589,897 + MMP_RS03130 0.21 -0.8 590,016 + MMP_RS03130 0.42 -2.6 590,031 - MMP_RS03130 0.44 -2.1 590,055 - MMP_RS03130 0.49 -0.9 590,060 - MMP_RS03130 0.50 -0.6 590,100 + MMP_RS03130 0.57 -0.5 590,100 + MMP_RS03130 0.57 -1.8 590,103 + MMP_RS03130 0.57 -1.4 590,267 - MMP_RS03130 0.86 -1.8 590,277 - MMP_RS03130 0.88 -0.7 590,323 - -1.1 590,387 - -3.5 590,401 + -2.3 590,446 - -2.5 590,528 - -5.0 590,730 + -3.5 590,905 - -2.0 590,983 + -3.7 591,114 - -2.4 591,321 - -1.2 591,340 - -2.4 591,341 + -1.6 591,341 + -3.0
Or see this region's nucleotide sequence