Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2218 and Rru_A2219 are separated by 169 nucleotides Rru_A2219 and Rru_A2220 are separated by 368 nucleotides Rru_A2220 and Rru_A2221 are separated by 71 nucleotides Rru_A2221 and Rru_A2222 are separated by 43 nucleotides
Rru_A2218: Rru_A2218 - Short-chain dehydrogenase/reductase SDR (NCBI), at 2,576,756 to 2,577,562
_A2218
Rru_A2219: Rru_A2219 - Dihydroneopterin aldolase family (NCBI), at 2,577,732 to 2,578,151
_A2219
Rru_A2220: Rru_A2220 - Excinuclease ABC, C subunit (NCBI), at 2,578,520 to 2,580,439
_A2220
Rru_A2221: Rru_A2221 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (NCBI), at 2,580,511 to 2,581,104
_A2221
Rru_A2222: Rru_A2222 - Molybdopterin biosynthesis protein (NCBI), at 2,581,148 to 2,582,434
_A2222
Position (kb)
2578
2579
2580
2581 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2577.560 kb on + strand at 2577.560 kb on + strand at 2577.635 kb on + strand at 2578.691 kb on + strand at 2578.691 kb on + strand at 2578.691 kb on + strand at 2578.691 kb on + strand at 2578.691 kb on + strand at 2578.733 kb on + strand, within Rru_A2220 at 2578.734 kb on - strand, within Rru_A2220 at 2578.813 kb on + strand, within Rru_A2220 at 2578.872 kb on - strand, within Rru_A2220 at 2578.907 kb on + strand, within Rru_A2220 at 2578.907 kb on + strand, within Rru_A2220 at 2578.907 kb on + strand, within Rru_A2220 at 2578.908 kb on - strand, within Rru_A2220 at 2578.909 kb on + strand, within Rru_A2220 at 2578.909 kb on + strand, within Rru_A2220 at 2578.909 kb on + strand, within Rru_A2220 at 2578.982 kb on + strand, within Rru_A2220 at 2578.983 kb on - strand, within Rru_A2220 at 2579.020 kb on + strand, within Rru_A2220 at 2579.020 kb on + strand, within Rru_A2220 at 2579.160 kb on - strand, within Rru_A2220 at 2579.160 kb on - strand, within Rru_A2220 at 2579.330 kb on - strand, within Rru_A2220 at 2579.330 kb on - strand, within Rru_A2220 at 2579.510 kb on + strand, within Rru_A2220 at 2579.511 kb on - strand, within Rru_A2220 at 2579.511 kb on - strand, within Rru_A2220 at 2579.783 kb on + strand, within Rru_A2220 at 2579.784 kb on - strand, within Rru_A2220 at 2579.784 kb on - strand, within Rru_A2220 at 2579.784 kb on - strand, within Rru_A2220 at 2579.784 kb on - strand, within Rru_A2220 at 2579.784 kb on - strand, within Rru_A2220 at 2579.797 kb on + strand, within Rru_A2220 at 2579.798 kb on - strand, within Rru_A2220 at 2579.798 kb on - strand, within Rru_A2220 at 2579.798 kb on - strand, within Rru_A2220 at 2579.798 kb on - strand, within Rru_A2220 at 2579.798 kb on - strand, within Rru_A2220 at 2579.913 kb on - strand, within Rru_A2220 at 2579.913 kb on - strand, within Rru_A2220 at 2579.913 kb on - strand, within Rru_A2220 at 2580.278 kb on - strand at 2580.413 kb on + strand at 2580.438 kb on - strand at 2580.485 kb on + strand at 2580.485 kb on + strand at 2580.486 kb on - strand at 2581.089 kb on + strand at 2581.092 kb on + strand at 2581.092 kb on + strand at 2581.093 kb on - strand at 2581.361 kb on - strand, within Rru_A2222 at 2581.361 kb on - strand, within Rru_A2222 at 2581.361 kb on - strand, within Rru_A2222 at 2581.379 kb on + strand, within Rru_A2222
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 2,577,560 + +2.7 2,577,560 + +3.4 2,577,635 + +2.6 2,578,691 + -0.4 2,578,691 + +0.6 2,578,691 + -2.0 2,578,691 + +2.1 2,578,691 + -0.7 2,578,733 + Rru_A2220 0.11 -0.2 2,578,734 - Rru_A2220 0.11 -3.5 2,578,813 + Rru_A2220 0.15 -2.9 2,578,872 - Rru_A2220 0.18 -1.1 2,578,907 + Rru_A2220 0.20 -2.5 2,578,907 + Rru_A2220 0.20 +0.4 2,578,907 + Rru_A2220 0.20 -4.6 2,578,908 - Rru_A2220 0.20 -2.9 2,578,909 + Rru_A2220 0.20 -3.2 2,578,909 + Rru_A2220 0.20 -0.6 2,578,909 + Rru_A2220 0.20 +0.7 2,578,982 + Rru_A2220 0.24 -1.0 2,578,983 - Rru_A2220 0.24 +0.4 2,579,020 + Rru_A2220 0.26 -0.8 2,579,020 + Rru_A2220 0.26 -4.5 2,579,160 - Rru_A2220 0.33 -3.8 2,579,160 - Rru_A2220 0.33 -0.4 2,579,330 - Rru_A2220 0.42 +1.0 2,579,330 - Rru_A2220 0.42 -1.4 2,579,510 + Rru_A2220 0.52 -4.4 2,579,511 - Rru_A2220 0.52 -1.8 2,579,511 - Rru_A2220 0.52 +2.2 2,579,783 + Rru_A2220 0.66 +1.0 2,579,784 - Rru_A2220 0.66 +1.3 2,579,784 - Rru_A2220 0.66 +1.2 2,579,784 - Rru_A2220 0.66 -0.6 2,579,784 - Rru_A2220 0.66 +1.1 2,579,784 - Rru_A2220 0.66 +0.6 2,579,797 + Rru_A2220 0.67 -0.8 2,579,798 - Rru_A2220 0.67 -2.0 2,579,798 - Rru_A2220 0.67 -1.4 2,579,798 - Rru_A2220 0.67 -2.3 2,579,798 - Rru_A2220 0.67 +0.7 2,579,798 - Rru_A2220 0.67 -0.6 2,579,913 - Rru_A2220 0.73 -1.3 2,579,913 - Rru_A2220 0.73 -4.2 2,579,913 - Rru_A2220 0.73 +1.2 2,580,278 - -0.2 2,580,413 + -1.2 2,580,438 - -2.0 2,580,485 + +1.5 2,580,485 + -0.1 2,580,486 - +0.7 2,581,089 + -0.5 2,581,092 + +0.1 2,581,092 + +0.4 2,581,093 - -1.7 2,581,361 - Rru_A2222 0.17 +1.0 2,581,361 - Rru_A2222 0.17 +0.2 2,581,361 - Rru_A2222 0.17 +1.0 2,581,379 + Rru_A2222 0.18 +0.2
Or see this region's nucleotide sequence