Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2153 and Rru_A2154 overlap by 4 nucleotides Rru_A2154 and Rru_A2155 overlap by 1 nucleotides Rru_A2155 and Rru_A2156 are separated by 442 nucleotides
Rru_A2153: Rru_A2153 - TonB-like (NCBI), at 2,501,387 to 2,502,217
_A2153
Rru_A2154: Rru_A2154 - Biopolymer transport protein ExbD/TolR (NCBI), at 2,502,214 to 2,502,681
_A2154
Rru_A2155: Rru_A2155 - MotA/TolQ/ExbB proton channel (NCBI), at 2,502,681 to 2,503,571
_A2155
Rru_A2156: Rru_A2156 - Enoyl-CoA hydratase/isomerase (NCBI), at 2,504,014 to 2,504,799
_A2156
Position (kb)
2502
2503
2504 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2501.849 kb on - strand, within Rru_A2153 at 2502.338 kb on + strand, within Rru_A2154 at 2502.365 kb on + strand, within Rru_A2154 at 2502.365 kb on + strand, within Rru_A2154 at 2502.365 kb on + strand, within Rru_A2154 at 2502.366 kb on - strand, within Rru_A2154 at 2502.366 kb on - strand, within Rru_A2154 at 2503.090 kb on + strand, within Rru_A2155 at 2503.090 kb on + strand, within Rru_A2155 at 2503.090 kb on + strand, within Rru_A2155 at 2503.090 kb on + strand, within Rru_A2155 at 2503.091 kb on - strand, within Rru_A2155 at 2503.091 kb on - strand, within Rru_A2155 at 2503.091 kb on - strand, within Rru_A2155 at 2503.566 kb on - strand at 2503.642 kb on + strand at 2503.642 kb on + strand at 2503.643 kb on - strand at 2503.710 kb on - strand at 2503.732 kb on + strand at 2503.732 kb on + strand at 2503.733 kb on - strand at 2503.733 kb on - strand at 2503.762 kb on + strand at 2503.766 kb on - strand at 2503.768 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 2,501,849 - Rru_A2153 0.56 -3.7 2,502,338 + Rru_A2154 0.26 -1.0 2,502,365 + Rru_A2154 0.32 -1.3 2,502,365 + Rru_A2154 0.32 +0.2 2,502,365 + Rru_A2154 0.32 -4.9 2,502,366 - Rru_A2154 0.32 -1.6 2,502,366 - Rru_A2154 0.32 -2.9 2,503,090 + Rru_A2155 0.46 -2.7 2,503,090 + Rru_A2155 0.46 -0.6 2,503,090 + Rru_A2155 0.46 +0.7 2,503,090 + Rru_A2155 0.46 +0.7 2,503,091 - Rru_A2155 0.46 +0.8 2,503,091 - Rru_A2155 0.46 +0.6 2,503,091 - Rru_A2155 0.46 +0.4 2,503,566 - -1.8 2,503,642 + -2.0 2,503,642 + -1.1 2,503,643 - +0.4 2,503,710 - +0.3 2,503,732 + -0.4 2,503,732 + -0.7 2,503,733 - +0.6 2,503,733 - -2.3 2,503,762 + -1.0 2,503,766 - -0.5 2,503,768 + -1.3
Or see this region's nucleotide sequence