Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1941 and Rru_A1942 overlap by 19 nucleotides Rru_A1942 and Rru_A1943 are separated by 13 nucleotides
Rru_A1941: Rru_A1941 - acyl-CoA carboxylase alpha chain (NCBI), at 2,248,388 to 2,250,448
_A1941
Rru_A1942: Rru_A1942 - Enoyl-CoA hydratase/isomerase (NCBI), at 2,250,430 to 2,251,257
_A1942
Rru_A1943: Rru_A1943 - Propionyl-CoA carboxylase (NCBI), at 2,251,271 to 2,252,878
_A1943
Position (kb)
2250
2251
2252 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2249.592 kb on - strand, within Rru_A1941 at 2249.598 kb on + strand, within Rru_A1941 at 2249.598 kb on + strand, within Rru_A1941 at 2249.599 kb on - strand, within Rru_A1941 at 2249.646 kb on + strand, within Rru_A1941 at 2249.906 kb on + strand, within Rru_A1941 at 2249.906 kb on + strand, within Rru_A1941 at 2249.907 kb on - strand, within Rru_A1941 at 2250.048 kb on + strand, within Rru_A1941 at 2250.049 kb on - strand, within Rru_A1941 at 2250.049 kb on - strand, within Rru_A1941 at 2250.270 kb on + strand at 2250.815 kb on + strand, within Rru_A1942 at 2250.815 kb on + strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.929 kb on + strand, within Rru_A1942 at 2250.929 kb on + strand, within Rru_A1942 at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.402 kb on - strand at 2251.404 kb on + strand at 2251.405 kb on - strand at 2251.405 kb on - strand at 2251.425 kb on + strand at 2251.426 kb on - strand at 2251.593 kb on + strand, within Rru_A1943 at 2251.615 kb on - strand, within Rru_A1943 at 2251.615 kb on - strand, within Rru_A1943 at 2251.911 kb on + strand, within Rru_A1943 at 2251.911 kb on + strand, within Rru_A1943 at 2251.977 kb on + strand, within Rru_A1943 at 2251.977 kb on + strand, within Rru_A1943 at 2251.978 kb on - strand, within Rru_A1943 at 2251.978 kb on - strand, within Rru_A1943 at 2252.028 kb on + strand, within Rru_A1943 at 2252.028 kb on + strand, within Rru_A1943 at 2252.028 kb on + strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.136 kb on + strand, within Rru_A1943 at 2252.136 kb on + strand, within Rru_A1943 at 2252.137 kb on - strand, within Rru_A1943
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 2,249,592 - Rru_A1941 0.58 -1.7 2,249,598 + Rru_A1941 0.59 +0.2 2,249,598 + Rru_A1941 0.59 -1.3 2,249,599 - Rru_A1941 0.59 +2.2 2,249,646 + Rru_A1941 0.61 -0.1 2,249,906 + Rru_A1941 0.74 -0.6 2,249,906 + Rru_A1941 0.74 -0.9 2,249,907 - Rru_A1941 0.74 +1.3 2,250,048 + Rru_A1941 0.81 -4.5 2,250,049 - Rru_A1941 0.81 +0.2 2,250,049 - Rru_A1941 0.81 -2.3 2,250,270 + +0.2 2,250,815 + Rru_A1942 0.46 +0.1 2,250,815 + Rru_A1942 0.46 -0.3 2,250,816 - Rru_A1942 0.47 +0.8 2,250,816 - Rru_A1942 0.47 +0.0 2,250,816 - Rru_A1942 0.47 +1.7 2,250,816 - Rru_A1942 0.47 -0.3 2,250,816 - Rru_A1942 0.47 -1.3 2,250,816 - Rru_A1942 0.47 -1.6 2,250,929 + Rru_A1942 0.60 -2.4 2,250,929 + Rru_A1942 0.60 -0.8 2,251,401 + +1.3 2,251,401 + +0.8 2,251,401 + -2.0 2,251,401 + -0.2 2,251,402 - +1.3 2,251,404 + -0.6 2,251,405 - -4.1 2,251,405 - +0.8 2,251,425 + -3.4 2,251,426 - +1.8 2,251,593 + Rru_A1943 0.20 -0.2 2,251,615 - Rru_A1943 0.21 +0.6 2,251,615 - Rru_A1943 0.21 -2.3 2,251,911 + Rru_A1943 0.40 -0.9 2,251,911 + Rru_A1943 0.40 +1.4 2,251,977 + Rru_A1943 0.44 +0.0 2,251,977 + Rru_A1943 0.44 -3.6 2,251,978 - Rru_A1943 0.44 -0.1 2,251,978 - Rru_A1943 0.44 -0.1 2,252,028 + Rru_A1943 0.47 +0.4 2,252,028 + Rru_A1943 0.47 +1.5 2,252,028 + Rru_A1943 0.47 +0.1 2,252,029 - Rru_A1943 0.47 +0.4 2,252,029 - Rru_A1943 0.47 -0.3 2,252,029 - Rru_A1943 0.47 -2.2 2,252,029 - Rru_A1943 0.47 -1.9 2,252,136 + Rru_A1943 0.54 -3.8 2,252,136 + Rru_A1943 0.54 -1.9 2,252,137 - Rru_A1943 0.54 +1.4
Or see this region's nucleotide sequence