Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1414 and Rru_A1415 are separated by 99 nucleotides Rru_A1415 and Rru_A1416 overlap by 4 nucleotides Rru_A1416 and Rru_A1417 are separated by 123 nucleotides Rru_A1417 and Rru_A1418 are separated by 139 nucleotides Rru_A1418 and Rru_A1419 are separated by 227 nucleotides
Rru_A1414: Rru_A1414 - regulatory protein GntR (NCBI), at 1,672,026 to 1,672,802
_A1414
Rru_A1415: Rru_A1415 - Protein of unknown function DUF132 (NCBI), at 1,672,902 to 1,673,366
_A1415
Rru_A1416: Rru_A1416 - hypothetical protein (NCBI), at 1,673,363 to 1,673,614
_A1416
Rru_A1417: Rru_A1417 - thioredoxin-disulfide reductase (NCBI), at 1,673,738 to 1,675,318
_A1417
Rru_A1418: Rru_A1418 - Alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen (NCBI), at 1,675,458 to 1,676,021
_A1418
Rru_A1419: Rru_A1419 - Carboxylyase-related protein (NCBI), at 1,676,249 to 1,677,640
_A1419
Position (kb)
1673
1674
1675
1676 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1672.851 kb on + strand at 1672.851 kb on + strand at 1672.857 kb on + strand at 1672.857 kb on + strand at 1672.857 kb on + strand at 1672.861 kb on + strand at 1673.036 kb on + strand, within Rru_A1415 at 1673.037 kb on - strand, within Rru_A1415 at 1673.037 kb on - strand, within Rru_A1415 at 1673.071 kb on - strand, within Rru_A1415 at 1673.117 kb on - strand, within Rru_A1415 at 1673.117 kb on - strand, within Rru_A1415 at 1673.273 kb on + strand, within Rru_A1415 at 1673.273 kb on + strand, within Rru_A1415 at 1673.274 kb on - strand, within Rru_A1415 at 1673.323 kb on + strand at 1673.839 kb on + strand at 1673.839 kb on + strand at 1673.839 kb on + strand at 1673.840 kb on - strand at 1673.840 kb on - strand at 1673.840 kb on - strand at 1673.842 kb on - strand at 1674.036 kb on + strand, within Rru_A1417 at 1674.036 kb on + strand, within Rru_A1417 at 1674.037 kb on - strand, within Rru_A1417 at 1674.037 kb on - strand, within Rru_A1417 at 1674.037 kb on - strand, within Rru_A1417 at 1674.312 kb on + strand, within Rru_A1417 at 1674.483 kb on + strand, within Rru_A1417 at 1674.484 kb on - strand, within Rru_A1417 at 1674.633 kb on + strand, within Rru_A1417 at 1674.633 kb on + strand, within Rru_A1417 at 1674.633 kb on + strand, within Rru_A1417 at 1674.634 kb on - strand, within Rru_A1417 at 1674.634 kb on - strand, within Rru_A1417 at 1674.634 kb on - strand, within Rru_A1417 at 1674.766 kb on + strand, within Rru_A1417 at 1674.798 kb on + strand, within Rru_A1417 at 1674.799 kb on - strand, within Rru_A1417 at 1674.799 kb on - strand, within Rru_A1417 at 1674.799 kb on - strand, within Rru_A1417 at 1674.799 kb on - strand, within Rru_A1417 at 1674.952 kb on - strand, within Rru_A1417 at 1674.952 kb on - strand, within Rru_A1417 at 1674.952 kb on - strand, within Rru_A1417 at 1674.964 kb on + strand, within Rru_A1417 at 1674.964 kb on + strand, within Rru_A1417 at 1674.965 kb on - strand, within Rru_A1417 at 1674.965 kb on - strand, within Rru_A1417 at 1675.281 kb on + strand at 1675.282 kb on - strand at 1675.459 kb on + strand at 1676.181 kb on - strand at 1676.233 kb on + strand at 1676.234 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 1,672,851 + +1.3 1,672,851 + -2.5 1,672,857 + +0.2 1,672,857 + -5.5 1,672,857 + +0.4 1,672,861 + -2.3 1,673,036 + Rru_A1415 0.29 +0.5 1,673,037 - Rru_A1415 0.29 -2.8 1,673,037 - Rru_A1415 0.29 +0.7 1,673,071 - Rru_A1415 0.36 +1.7 1,673,117 - Rru_A1415 0.46 +0.9 1,673,117 - Rru_A1415 0.46 +0.6 1,673,273 + Rru_A1415 0.80 +0.3 1,673,273 + Rru_A1415 0.80 -0.7 1,673,274 - Rru_A1415 0.80 +0.5 1,673,323 + -0.4 1,673,839 + +0.6 1,673,839 + -2.1 1,673,839 + +0.9 1,673,840 - -0.5 1,673,840 - -3.3 1,673,840 - -2.1 1,673,842 - +0.5 1,674,036 + Rru_A1417 0.19 -2.2 1,674,036 + Rru_A1417 0.19 -0.5 1,674,037 - Rru_A1417 0.19 +1.2 1,674,037 - Rru_A1417 0.19 -3.1 1,674,037 - Rru_A1417 0.19 +0.6 1,674,312 + Rru_A1417 0.36 -1.8 1,674,483 + Rru_A1417 0.47 -1.5 1,674,484 - Rru_A1417 0.47 -1.7 1,674,633 + Rru_A1417 0.57 +0.5 1,674,633 + Rru_A1417 0.57 -1.6 1,674,633 + Rru_A1417 0.57 -1.8 1,674,634 - Rru_A1417 0.57 -0.1 1,674,634 - Rru_A1417 0.57 +1.5 1,674,634 - Rru_A1417 0.57 +0.2 1,674,766 + Rru_A1417 0.65 -2.6 1,674,798 + Rru_A1417 0.67 -0.5 1,674,799 - Rru_A1417 0.67 +1.5 1,674,799 - Rru_A1417 0.67 +2.5 1,674,799 - Rru_A1417 0.67 -1.4 1,674,799 - Rru_A1417 0.67 +1.5 1,674,952 - Rru_A1417 0.77 +2.5 1,674,952 - Rru_A1417 0.77 -0.5 1,674,952 - Rru_A1417 0.77 -1.2 1,674,964 + Rru_A1417 0.78 +1.5 1,674,964 + Rru_A1417 0.78 +0.5 1,674,965 - Rru_A1417 0.78 -0.7 1,674,965 - Rru_A1417 0.78 -1.8 1,675,281 + +1.5 1,675,282 - -0.7 1,675,459 + -0.8 1,676,181 - +0.9 1,676,233 + +0.9 1,676,234 - -0.7
Or see this region's nucleotide sequence