Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0444 and Rru_A0445 overlap by 4 nucleotides Rru_A0445 and Rru_A0446 are separated by 186 nucleotides Rru_A0446 and Rru_A0447 are separated by 13 nucleotides
Rru_A0444: Rru_A0444 - Sua5/YciO/YrdC/YwlC (NCBI), at 522,974 to 523,942
_A0444
Rru_A0445: Rru_A0445 - 3(2),5 -bisphosphate nucleotidase (NCBI), at 523,939 to 524,721
_A0445
Rru_A0446: Rru_A0446 - hypothetical protein (NCBI), at 524,908 to 525,354
_A0446
Rru_A0447: Rru_A0447 - AMMECR1 (NCBI), at 525,368 to 526,132
_A0447
Position (kb) 523
524
525 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 522.970 kb on - strand at 524.928 kb on - strand at 524.928 kb on - strand at 524.970 kb on + strand, within Rru_A0446 at 524.970 kb on + strand, within Rru_A0446 at 524.970 kb on + strand, within Rru_A0446 at 524.970 kb on + strand, within Rru_A0446 at 524.971 kb on - strand, within Rru_A0446 at 524.971 kb on - strand, within Rru_A0446 at 524.971 kb on - strand, within Rru_A0446 at 524.999 kb on + strand, within Rru_A0446 at 524.999 kb on + strand, within Rru_A0446 at 525.045 kb on - strand, within Rru_A0446 at 525.062 kb on + strand, within Rru_A0446 at 525.062 kb on + strand, within Rru_A0446 at 525.063 kb on - strand, within Rru_A0446 at 525.063 kb on - strand, within Rru_A0446 at 525.063 kb on - strand, within Rru_A0446 at 525.063 kb on - strand, within Rru_A0446 at 525.454 kb on - strand, within Rru_A0447 at 525.454 kb on - strand, within Rru_A0447 at 525.454 kb on - strand, within Rru_A0447 at 525.475 kb on + strand, within Rru_A0447 at 525.475 kb on + strand, within Rru_A0447 at 525.476 kb on - strand, within Rru_A0447 at 525.481 kb on + strand, within Rru_A0447 at 525.481 kb on + strand, within Rru_A0447 at 525.481 kb on + strand, within Rru_A0447 at 525.482 kb on - strand, within Rru_A0447 at 525.482 kb on - strand, within Rru_A0447
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 522,970 - +1.0 524,928 - -5.2 524,928 - +1.4 524,970 + Rru_A0446 0.14 +0.8 524,970 + Rru_A0446 0.14 +0.6 524,970 + Rru_A0446 0.14 -1.9 524,970 + Rru_A0446 0.14 -2.4 524,971 - Rru_A0446 0.14 +0.6 524,971 - Rru_A0446 0.14 +1.6 524,971 - Rru_A0446 0.14 -0.6 524,999 + Rru_A0446 0.20 -0.3 524,999 + Rru_A0446 0.20 +1.2 525,045 - Rru_A0446 0.31 +0.1 525,062 + Rru_A0446 0.34 -1.2 525,062 + Rru_A0446 0.34 -2.7 525,063 - Rru_A0446 0.35 +1.3 525,063 - Rru_A0446 0.35 +0.1 525,063 - Rru_A0446 0.35 -0.6 525,063 - Rru_A0446 0.35 -3.3 525,454 - Rru_A0447 0.11 -1.0 525,454 - Rru_A0447 0.11 -1.4 525,454 - Rru_A0447 0.11 -1.4 525,475 + Rru_A0447 0.14 -0.4 525,475 + Rru_A0447 0.14 -1.5 525,476 - Rru_A0447 0.14 +1.0 525,481 + Rru_A0447 0.15 -4.1 525,481 + Rru_A0447 0.15 -1.4 525,481 + Rru_A0447 0.15 -1.0 525,482 - Rru_A0447 0.15 +2.0 525,482 - Rru_A0447 0.15 +0.9
Or see this region's nucleotide sequence