Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0232 and Rru_A0233 are separated by 144 nucleotides Rru_A0233 and Rru_A0234 overlap by 4 nucleotides Rru_A0234 and Rru_A0235 are separated by 254 nucleotides
Rru_A0232: Rru_A0232 - hypothetical protein (NCBI), at 285,307 to 288,021
_A0232
Rru_A0233: Rru_A0233 - hypothetical protein (NCBI), at 288,166 to 289,485
_A0233
Rru_A0234: Rru_A0234 - hypothetical protein (NCBI), at 289,482 to 290,006
_A0234
Rru_A0235: Rru_A0235 - preprotein translocase subunit SecA (RefSeq), at 290,261 to 292,951
_A0235
Position (kb) 288
289
290 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 287.242 kb on + strand, within Rru_A0232 at 287.360 kb on + strand, within Rru_A0232 at 287.435 kb on - strand, within Rru_A0232 at 287.783 kb on - strand at 287.842 kb on + strand at 287.843 kb on - strand at 287.843 kb on - strand at 288.109 kb on + strand at 288.109 kb on + strand at 288.167 kb on + strand at 288.168 kb on - strand at 288.168 kb on - strand at 288.179 kb on - strand at 288.223 kb on + strand at 288.224 kb on - strand at 288.224 kb on - strand at 288.247 kb on - strand at 288.247 kb on - strand at 288.485 kb on - strand, within Rru_A0233 at 288.485 kb on - strand, within Rru_A0233 at 288.612 kb on + strand, within Rru_A0233 at 288.612 kb on + strand, within Rru_A0233 at 288.613 kb on - strand, within Rru_A0233 at 288.613 kb on - strand, within Rru_A0233 at 288.879 kb on + strand, within Rru_A0233 at 288.880 kb on - strand, within Rru_A0233 at 288.880 kb on - strand, within Rru_A0233 at 288.970 kb on + strand, within Rru_A0233 at 288.970 kb on + strand, within Rru_A0233 at 288.970 kb on + strand, within Rru_A0233 at 288.971 kb on - strand, within Rru_A0233 at 289.103 kb on + strand, within Rru_A0233 at 289.103 kb on + strand, within Rru_A0233 at 289.104 kb on - strand, within Rru_A0233 at 289.104 kb on - strand, within Rru_A0233 at 289.104 kb on - strand, within Rru_A0233 at 289.172 kb on + strand, within Rru_A0233 at 289.172 kb on + strand, within Rru_A0233 at 289.172 kb on + strand, within Rru_A0233 at 289.172 kb on + strand, within Rru_A0233 at 289.172 kb on + strand, within Rru_A0233 at 289.172 kb on + strand, within Rru_A0233 at 289.172 kb on + strand, within Rru_A0233 at 289.172 kb on + strand, within Rru_A0233 at 289.173 kb on - strand, within Rru_A0233 at 289.173 kb on - strand, within Rru_A0233 at 289.173 kb on - strand, within Rru_A0233 at 289.173 kb on - strand, within Rru_A0233 at 289.173 kb on - strand, within Rru_A0233 at 289.360 kb on + strand at 289.361 kb on - strand at 289.405 kb on + strand at 289.405 kb on + strand at 289.405 kb on + strand at 289.406 kb on - strand at 289.406 kb on - strand at 289.406 kb on - strand at 289.460 kb on - strand at 289.490 kb on - strand at 289.544 kb on - strand, within Rru_A0234 at 289.549 kb on + strand, within Rru_A0234 at 289.559 kb on - strand, within Rru_A0234 at 289.615 kb on + strand, within Rru_A0234 at 289.616 kb on - strand, within Rru_A0234 at 289.616 kb on - strand, within Rru_A0234 at 289.616 kb on - strand, within Rru_A0234 at 289.652 kb on - strand, within Rru_A0234 at 289.801 kb on + strand, within Rru_A0234 at 289.862 kb on + strand, within Rru_A0234 at 289.862 kb on + strand, within Rru_A0234 at 289.899 kb on + strand, within Rru_A0234 at 289.899 kb on + strand, within Rru_A0234 at 289.900 kb on - strand, within Rru_A0234 at 289.900 kb on - strand, within Rru_A0234 at 290.030 kb on + strand at 290.030 kb on + strand at 290.031 kb on - strand at 290.040 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 287,242 + Rru_A0232 0.71 -4.1 287,360 + Rru_A0232 0.76 -0.9 287,435 - Rru_A0232 0.78 -2.6 287,783 - -4.5 287,842 + -0.9 287,843 - -1.2 287,843 - +0.3 288,109 + -0.2 288,109 + -3.8 288,167 + -1.2 288,168 - -1.3 288,168 - -4.5 288,179 - +0.0 288,223 + -0.2 288,224 - -2.2 288,224 - -1.6 288,247 - -2.8 288,247 - -0.5 288,485 - Rru_A0233 0.24 +1.8 288,485 - Rru_A0233 0.24 +0.3 288,612 + Rru_A0233 0.34 +0.1 288,612 + Rru_A0233 0.34 +0.5 288,613 - Rru_A0233 0.34 +2.0 288,613 - Rru_A0233 0.34 +0.6 288,879 + Rru_A0233 0.54 -2.0 288,880 - Rru_A0233 0.54 +1.2 288,880 - Rru_A0233 0.54 -1.1 288,970 + Rru_A0233 0.61 -1.1 288,970 + Rru_A0233 0.61 +1.2 288,970 + Rru_A0233 0.61 -0.6 288,971 - Rru_A0233 0.61 +0.2 289,103 + Rru_A0233 0.71 -0.4 289,103 + Rru_A0233 0.71 -0.4 289,104 - Rru_A0233 0.71 -3.4 289,104 - Rru_A0233 0.71 -0.3 289,104 - Rru_A0233 0.71 +0.8 289,172 + Rru_A0233 0.76 -0.8 289,172 + Rru_A0233 0.76 +1.3 289,172 + Rru_A0233 0.76 -4.6 289,172 + Rru_A0233 0.76 -1.2 289,172 + Rru_A0233 0.76 -0.8 289,172 + Rru_A0233 0.76 +0.9 289,172 + Rru_A0233 0.76 -0.0 289,172 + Rru_A0233 0.76 +0.4 289,173 - Rru_A0233 0.76 +2.1 289,173 - Rru_A0233 0.76 -1.1 289,173 - Rru_A0233 0.76 -0.1 289,173 - Rru_A0233 0.76 +0.8 289,173 - Rru_A0233 0.76 -0.8 289,360 + -1.5 289,361 - -0.1 289,405 + -1.6 289,405 + +0.7 289,405 + +0.2 289,406 - +0.4 289,406 - +0.4 289,406 - -0.9 289,460 - -0.5 289,490 - -0.8 289,544 - Rru_A0234 0.12 -3.2 289,549 + Rru_A0234 0.13 +1.2 289,559 - Rru_A0234 0.15 -3.2 289,615 + Rru_A0234 0.25 +0.2 289,616 - Rru_A0234 0.26 -2.4 289,616 - Rru_A0234 0.26 -0.8 289,616 - Rru_A0234 0.26 +0.4 289,652 - Rru_A0234 0.32 +1.4 289,801 + Rru_A0234 0.61 +1.3 289,862 + Rru_A0234 0.72 -3.4 289,862 + Rru_A0234 0.72 -2.4 289,899 + Rru_A0234 0.79 -0.2 289,899 + Rru_A0234 0.79 +1.1 289,900 - Rru_A0234 0.80 +0.7 289,900 - Rru_A0234 0.80 +1.3 290,030 + -4.4 290,030 + +1.0 290,031 - -2.4 290,040 + +0.1
Or see this region's nucleotide sequence