Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0198 and Rru_A0199 overlap by 27 nucleotides Rru_A0199 and Rru_A0200 are separated by 167 nucleotides Rru_A0200 and Rru_A0201 are separated by 179 nucleotides Rru_A0201 and Rru_A0202 overlap by 4 nucleotides
Rru_A0198: Rru_A0198 - hypothetical protein (NCBI), at 246,145 to 248,130
_A0198
Rru_A0199: Rru_A0199 - hypothetical protein (NCBI), at 248,104 to 248,298
_A0199
Rru_A0200: Rru_A0200 - Restriction endonuclease (NCBI), at 248,466 to 249,677
_A0200
Rru_A0201: Rru_A0201 - Endonuclease/exonuclease/phosphatase (NCBI), at 249,857 to 250,651
_A0201
Rru_A0202: Rru_A0202 - Phospholipase D/Transphosphatidylase (NCBI), at 250,648 to 253,059
_A0202
Position (kb) 248
249
250 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 247.727 kb on + strand, within Rru_A0198 at 247.836 kb on + strand, within Rru_A0198 at 247.837 kb on - strand, within Rru_A0198 at 247.859 kb on + strand, within Rru_A0198 at 248.128 kb on + strand at 248.128 kb on + strand at 248.235 kb on + strand, within Rru_A0199 at 248.235 kb on + strand, within Rru_A0199 at 248.235 kb on + strand, within Rru_A0199 at 248.236 kb on - strand, within Rru_A0199 at 248.332 kb on + strand at 248.332 kb on + strand at 248.333 kb on - strand at 248.333 kb on - strand at 248.333 kb on - strand at 248.333 kb on - strand at 248.372 kb on + strand at 248.373 kb on - strand at 248.416 kb on + strand at 248.416 kb on + strand at 248.421 kb on - strand at 248.614 kb on - strand, within Rru_A0200 at 248.682 kb on - strand, within Rru_A0200 at 248.695 kb on + strand, within Rru_A0200 at 248.696 kb on - strand, within Rru_A0200 at 248.737 kb on + strand, within Rru_A0200 at 248.789 kb on + strand, within Rru_A0200 at 248.789 kb on + strand, within Rru_A0200 at 248.789 kb on + strand, within Rru_A0200 at 248.789 kb on + strand, within Rru_A0200 at 248.789 kb on + strand, within Rru_A0200 at 248.790 kb on - strand, within Rru_A0200 at 248.790 kb on - strand, within Rru_A0200 at 248.793 kb on + strand, within Rru_A0200 at 248.835 kb on + strand, within Rru_A0200 at 248.855 kb on - strand, within Rru_A0200 at 248.867 kb on - strand, within Rru_A0200 at 248.977 kb on + strand, within Rru_A0200 at 248.996 kb on + strand, within Rru_A0200 at 248.997 kb on - strand, within Rru_A0200 at 248.997 kb on - strand, within Rru_A0200 at 248.997 kb on - strand, within Rru_A0200 at 249.073 kb on + strand, within Rru_A0200 at 249.074 kb on - strand, within Rru_A0200 at 249.074 kb on - strand, within Rru_A0200 at 249.074 kb on - strand, within Rru_A0200 at 249.118 kb on + strand, within Rru_A0200 at 249.118 kb on + strand, within Rru_A0200 at 249.118 kb on + strand, within Rru_A0200 at 249.119 kb on - strand, within Rru_A0200 at 249.119 kb on - strand, within Rru_A0200 at 249.122 kb on + strand, within Rru_A0200 at 249.122 kb on + strand, within Rru_A0200 at 249.123 kb on - strand, within Rru_A0200 at 249.123 kb on - strand, within Rru_A0200 at 249.123 kb on - strand, within Rru_A0200 at 249.201 kb on + strand, within Rru_A0200 at 249.202 kb on - strand, within Rru_A0200 at 249.205 kb on + strand, within Rru_A0200 at 249.206 kb on - strand, within Rru_A0200 at 249.257 kb on + strand, within Rru_A0200 at 249.258 kb on - strand, within Rru_A0200 at 249.258 kb on - strand, within Rru_A0200 at 249.305 kb on + strand, within Rru_A0200 at 249.306 kb on - strand, within Rru_A0200 at 249.385 kb on + strand, within Rru_A0200 at 249.390 kb on + strand, within Rru_A0200 at 249.454 kb on - strand, within Rru_A0200 at 249.454 kb on - strand, within Rru_A0200 at 249.529 kb on + strand, within Rru_A0200 at 249.530 kb on - strand, within Rru_A0200 at 249.536 kb on + strand, within Rru_A0200 at 249.717 kb on - strand at 249.773 kb on - strand at 250.292 kb on + strand, within Rru_A0201 at 250.292 kb on + strand, within Rru_A0201 at 250.293 kb on - strand, within Rru_A0201 at 250.293 kb on - strand, within Rru_A0201 at 250.472 kb on + strand, within Rru_A0201 at 250.472 kb on + strand, within Rru_A0201 at 250.472 kb on + strand, within Rru_A0201 at 250.473 kb on - strand, within Rru_A0201 at 250.473 kb on - strand, within Rru_A0201
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 247,727 + Rru_A0198 0.80 -1.1 247,836 + Rru_A0198 0.85 +0.3 247,837 - Rru_A0198 0.85 +0.4 247,859 + Rru_A0198 0.86 +0.6 248,128 + -1.9 248,128 + -3.2 248,235 + Rru_A0199 0.67 -2.4 248,235 + Rru_A0199 0.67 -0.4 248,235 + Rru_A0199 0.67 -2.6 248,236 - Rru_A0199 0.68 +0.5 248,332 + +0.6 248,332 + -0.1 248,333 - -1.1 248,333 - -2.4 248,333 - +1.8 248,333 - -2.7 248,372 + -0.2 248,373 - +0.8 248,416 + +1.5 248,416 + -0.6 248,421 - +0.4 248,614 - Rru_A0200 0.12 +0.2 248,682 - Rru_A0200 0.18 -1.3 248,695 + Rru_A0200 0.19 +1.0 248,696 - Rru_A0200 0.19 -1.3 248,737 + Rru_A0200 0.22 -5.6 248,789 + Rru_A0200 0.27 -0.7 248,789 + Rru_A0200 0.27 +0.2 248,789 + Rru_A0200 0.27 -0.4 248,789 + Rru_A0200 0.27 -3.4 248,789 + Rru_A0200 0.27 -1.5 248,790 - Rru_A0200 0.27 -0.0 248,790 - Rru_A0200 0.27 -0.2 248,793 + Rru_A0200 0.27 -1.8 248,835 + Rru_A0200 0.30 +2.8 248,855 - Rru_A0200 0.32 -1.4 248,867 - Rru_A0200 0.33 +0.5 248,977 + Rru_A0200 0.42 -3.2 248,996 + Rru_A0200 0.44 -1.5 248,997 - Rru_A0200 0.44 +0.8 248,997 - Rru_A0200 0.44 -0.5 248,997 - Rru_A0200 0.44 +3.0 249,073 + Rru_A0200 0.50 +0.0 249,074 - Rru_A0200 0.50 -0.5 249,074 - Rru_A0200 0.50 +0.6 249,074 - Rru_A0200 0.50 -1.1 249,118 + Rru_A0200 0.54 -2.0 249,118 + Rru_A0200 0.54 +1.2 249,118 + Rru_A0200 0.54 -1.3 249,119 - Rru_A0200 0.54 +0.6 249,119 - Rru_A0200 0.54 +0.1 249,122 + Rru_A0200 0.54 +0.5 249,122 + Rru_A0200 0.54 -3.0 249,123 - Rru_A0200 0.54 +0.8 249,123 - Rru_A0200 0.54 +0.2 249,123 - Rru_A0200 0.54 -1.7 249,201 + Rru_A0200 0.61 -1.8 249,202 - Rru_A0200 0.61 -1.0 249,205 + Rru_A0200 0.61 +0.5 249,206 - Rru_A0200 0.61 -2.4 249,257 + Rru_A0200 0.65 -0.5 249,258 - Rru_A0200 0.65 -1.9 249,258 - Rru_A0200 0.65 -1.4 249,305 + Rru_A0200 0.69 -0.8 249,306 - Rru_A0200 0.69 -2.5 249,385 + Rru_A0200 0.76 -3.9 249,390 + Rru_A0200 0.76 -0.4 249,454 - Rru_A0200 0.82 +0.1 249,454 - Rru_A0200 0.82 +1.5 249,529 + Rru_A0200 0.88 +0.3 249,530 - Rru_A0200 0.88 -2.1 249,536 + Rru_A0200 0.88 +1.3 249,717 - -2.5 249,773 - -2.7 250,292 + Rru_A0201 0.55 -0.5 250,292 + Rru_A0201 0.55 +0.2 250,293 - Rru_A0201 0.55 +0.4 250,293 - Rru_A0201 0.55 -4.1 250,472 + Rru_A0201 0.77 -1.7 250,472 + Rru_A0201 0.77 -1.3 250,472 + Rru_A0201 0.77 +0.2 250,473 - Rru_A0201 0.77 +0.3 250,473 - Rru_A0201 0.77 -1.2
Or see this region's nucleotide sequence