Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0189 and Rru_A0190 are separated by 227 nucleotides Rru_A0190 and Rru_A0191 are separated by 367 nucleotides Rru_A0191 and Rru_A0192 are separated by 97 nucleotides
Rru_A0189: Rru_A0189 - ABC transporter, transmembrane region (NCBI), at 228,812 to 230,593
_A0189
Rru_A0190: Rru_A0190 - TonB-dependent receptor (NCBI), at 230,821 to 232,953
_A0190
Rru_A0191: Rru_A0191 - Aspartate 1-decarboxylase-like (NCBI), at 233,321 to 233,728
_A0191
Rru_A0192: Rru_A0192 - Multi antimicrobial extrusion protein MatE (NCBI), at 233,826 to 235,253
_A0192
Position (kb) 230
231
232
233 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 229.910 kb on - strand, within Rru_A0189 at 229.910 kb on - strand, within Rru_A0189 at 229.910 kb on - strand, within Rru_A0189 at 229.917 kb on + strand, within Rru_A0189 at 230.040 kb on + strand, within Rru_A0189 at 230.040 kb on + strand, within Rru_A0189 at 230.040 kb on + strand, within Rru_A0189 at 230.041 kb on - strand, within Rru_A0189 at 230.041 kb on - strand, within Rru_A0189 at 230.041 kb on - strand, within Rru_A0189 at 230.073 kb on - strand, within Rru_A0189 at 230.073 kb on - strand, within Rru_A0189 at 230.131 kb on + strand, within Rru_A0189 at 230.131 kb on + strand, within Rru_A0189 at 230.131 kb on + strand, within Rru_A0189 at 230.131 kb on + strand, within Rru_A0189 at 230.132 kb on - strand, within Rru_A0189 at 230.132 kb on - strand, within Rru_A0189 at 230.132 kb on - strand, within Rru_A0189 at 230.346 kb on + strand, within Rru_A0189 at 230.484 kb on + strand at 230.485 kb on - strand at 230.570 kb on + strand at 230.822 kb on + strand at 230.891 kb on + strand at 230.897 kb on + strand at 230.897 kb on + strand at 230.897 kb on + strand at 230.898 kb on - strand at 230.927 kb on + strand at 230.999 kb on + strand at 230.999 kb on + strand at 230.999 kb on + strand at 230.999 kb on + strand at 230.999 kb on + strand at 230.999 kb on + strand at 231.000 kb on - strand at 231.000 kb on - strand at 231.000 kb on - strand at 231.000 kb on - strand at 231.098 kb on + strand, within Rru_A0190 at 231.098 kb on + strand, within Rru_A0190 at 231.116 kb on + strand, within Rru_A0190 at 231.117 kb on - strand, within Rru_A0190 at 231.195 kb on + strand, within Rru_A0190 at 231.195 kb on + strand, within Rru_A0190 at 231.196 kb on - strand, within Rru_A0190 at 231.197 kb on + strand, within Rru_A0190 at 231.197 kb on + strand, within Rru_A0190 at 231.197 kb on + strand, within Rru_A0190 at 231.197 kb on + strand, within Rru_A0190 at 231.197 kb on + strand, within Rru_A0190 at 231.197 kb on + strand, within Rru_A0190 at 231.197 kb on + strand, within Rru_A0190 at 231.198 kb on - strand, within Rru_A0190 at 231.198 kb on - strand, within Rru_A0190 at 231.198 kb on - strand, within Rru_A0190 at 231.359 kb on + strand, within Rru_A0190 at 231.359 kb on + strand, within Rru_A0190 at 231.360 kb on - strand, within Rru_A0190 at 231.362 kb on + strand, within Rru_A0190 at 231.411 kb on - strand, within Rru_A0190 at 231.422 kb on + strand, within Rru_A0190 at 231.422 kb on + strand, within Rru_A0190 at 231.456 kb on + strand, within Rru_A0190 at 231.457 kb on - strand, within Rru_A0190 at 231.457 kb on - strand, within Rru_A0190 at 231.515 kb on + strand, within Rru_A0190 at 231.516 kb on - strand, within Rru_A0190 at 231.543 kb on + strand, within Rru_A0190 at 231.563 kb on + strand, within Rru_A0190 at 231.563 kb on + strand, within Rru_A0190 at 231.564 kb on - strand, within Rru_A0190 at 231.593 kb on + strand, within Rru_A0190 at 231.593 kb on + strand, within Rru_A0190 at 231.594 kb on - strand, within Rru_A0190 at 231.594 kb on - strand, within Rru_A0190 at 231.632 kb on + strand, within Rru_A0190 at 231.632 kb on + strand, within Rru_A0190 at 231.632 kb on + strand, within Rru_A0190 at 231.633 kb on - strand, within Rru_A0190 at 231.636 kb on + strand, within Rru_A0190 at 231.636 kb on + strand, within Rru_A0190 at 231.637 kb on - strand, within Rru_A0190 at 231.637 kb on - strand, within Rru_A0190 at 231.637 kb on - strand, within Rru_A0190 at 231.722 kb on + strand, within Rru_A0190 at 231.723 kb on - strand, within Rru_A0190 at 231.807 kb on - strand, within Rru_A0190 at 231.821 kb on + strand, within Rru_A0190 at 231.821 kb on + strand, within Rru_A0190 at 231.821 kb on + strand, within Rru_A0190 at 231.822 kb on - strand, within Rru_A0190 at 231.950 kb on + strand, within Rru_A0190 at 232.006 kb on + strand, within Rru_A0190 at 232.055 kb on + strand, within Rru_A0190 at 232.085 kb on + strand, within Rru_A0190 at 232.085 kb on + strand, within Rru_A0190 at 232.086 kb on - strand, within Rru_A0190 at 232.133 kb on + strand, within Rru_A0190 at 232.241 kb on + strand, within Rru_A0190 at 232.241 kb on + strand, within Rru_A0190 at 232.241 kb on + strand, within Rru_A0190 at 232.241 kb on + strand, within Rru_A0190 at 232.242 kb on - strand, within Rru_A0190 at 232.263 kb on + strand, within Rru_A0190 at 232.264 kb on - strand, within Rru_A0190 at 232.264 kb on - strand, within Rru_A0190 at 232.436 kb on + strand, within Rru_A0190 at 232.436 kb on + strand, within Rru_A0190 at 232.487 kb on + strand, within Rru_A0190 at 232.488 kb on - strand, within Rru_A0190 at 232.488 kb on - strand, within Rru_A0190 at 232.488 kb on - strand, within Rru_A0190 at 232.490 kb on + strand, within Rru_A0190 at 232.490 kb on + strand, within Rru_A0190 at 232.491 kb on - strand, within Rru_A0190 at 232.491 kb on - strand, within Rru_A0190 at 232.491 kb on - strand, within Rru_A0190 at 232.491 kb on - strand, within Rru_A0190 at 232.491 kb on - strand, within Rru_A0190 at 232.491 kb on - strand, within Rru_A0190 at 232.532 kb on + strand, within Rru_A0190 at 232.532 kb on + strand, within Rru_A0190 at 232.532 kb on + strand, within Rru_A0190 at 232.532 kb on + strand, within Rru_A0190 at 232.532 kb on + strand, within Rru_A0190 at 232.533 kb on - strand, within Rru_A0190 at 232.533 kb on - strand, within Rru_A0190 at 232.533 kb on - strand, within Rru_A0190 at 233.022 kb on + strand at 233.069 kb on + strand at 233.070 kb on - strand at 233.081 kb on + strand at 233.081 kb on + strand at 233.081 kb on + strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.082 kb on - strand at 233.115 kb on - strand at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.517 kb on + strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.518 kb on - strand, within Rru_A0191 at 233.635 kb on + strand, within Rru_A0191 at 233.635 kb on + strand, within Rru_A0191 at 233.635 kb on + strand, within Rru_A0191 at 233.636 kb on - strand, within Rru_A0191 at 233.636 kb on - strand, within Rru_A0191 at 233.681 kb on - strand, within Rru_A0191 at 233.681 kb on - strand, within Rru_A0191 at 233.681 kb on - strand, within Rru_A0191 at 233.750 kb on + strand at 233.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 229,910 - Rru_A0189 0.62 -0.0 229,910 - Rru_A0189 0.62 -2.7 229,910 - Rru_A0189 0.62 -0.7 229,917 + Rru_A0189 0.62 -1.2 230,040 + Rru_A0189 0.69 -3.5 230,040 + Rru_A0189 0.69 -0.9 230,040 + Rru_A0189 0.69 -2.2 230,041 - Rru_A0189 0.69 -2.8 230,041 - Rru_A0189 0.69 -0.6 230,041 - Rru_A0189 0.69 -1.0 230,073 - Rru_A0189 0.71 -0.8 230,073 - Rru_A0189 0.71 -1.2 230,131 + Rru_A0189 0.74 -2.5 230,131 + Rru_A0189 0.74 +0.5 230,131 + Rru_A0189 0.74 -3.3 230,131 + Rru_A0189 0.74 -2.2 230,132 - Rru_A0189 0.74 -2.4 230,132 - Rru_A0189 0.74 -2.1 230,132 - Rru_A0189 0.74 -2.6 230,346 + Rru_A0189 0.86 +0.3 230,484 + -1.7 230,485 - -1.1 230,570 + -1.9 230,822 + +2.5 230,891 + -0.0 230,897 + -1.2 230,897 + -3.8 230,897 + -0.5 230,898 - -1.9 230,927 + +1.1 230,999 + +0.4 230,999 + +0.4 230,999 + +0.2 230,999 + -1.4 230,999 + -2.2 230,999 + +1.0 231,000 - +0.2 231,000 - -0.8 231,000 - -2.3 231,000 - -1.0 231,098 + Rru_A0190 0.13 -0.6 231,098 + Rru_A0190 0.13 -2.5 231,116 + Rru_A0190 0.14 -2.0 231,117 - Rru_A0190 0.14 -1.7 231,195 + Rru_A0190 0.18 -0.4 231,195 + Rru_A0190 0.18 -1.9 231,196 - Rru_A0190 0.18 -1.6 231,197 + Rru_A0190 0.18 -1.9 231,197 + Rru_A0190 0.18 +0.2 231,197 + Rru_A0190 0.18 -0.4 231,197 + Rru_A0190 0.18 -1.9 231,197 + Rru_A0190 0.18 +0.6 231,197 + Rru_A0190 0.18 -0.1 231,197 + Rru_A0190 0.18 +0.9 231,198 - Rru_A0190 0.18 -1.1 231,198 - Rru_A0190 0.18 +1.8 231,198 - Rru_A0190 0.18 -0.2 231,359 + Rru_A0190 0.25 +0.1 231,359 + Rru_A0190 0.25 +0.2 231,360 - Rru_A0190 0.25 +1.5 231,362 + Rru_A0190 0.25 +0.5 231,411 - Rru_A0190 0.28 +0.8 231,422 + Rru_A0190 0.28 +2.0 231,422 + Rru_A0190 0.28 -1.0 231,456 + Rru_A0190 0.30 +0.4 231,457 - Rru_A0190 0.30 -1.9 231,457 - Rru_A0190 0.30 +0.7 231,515 + Rru_A0190 0.33 +0.4 231,516 - Rru_A0190 0.33 -2.1 231,543 + Rru_A0190 0.34 -0.1 231,563 + Rru_A0190 0.35 -3.8 231,563 + Rru_A0190 0.35 -3.7 231,564 - Rru_A0190 0.35 +0.2 231,593 + Rru_A0190 0.36 -0.6 231,593 + Rru_A0190 0.36 -4.2 231,594 - Rru_A0190 0.36 -1.8 231,594 - Rru_A0190 0.36 +0.6 231,632 + Rru_A0190 0.38 +0.8 231,632 + Rru_A0190 0.38 -0.3 231,632 + Rru_A0190 0.38 +1.3 231,633 - Rru_A0190 0.38 +1.1 231,636 + Rru_A0190 0.38 -1.3 231,636 + Rru_A0190 0.38 -4.2 231,637 - Rru_A0190 0.38 -3.2 231,637 - Rru_A0190 0.38 +0.1 231,637 - Rru_A0190 0.38 -0.1 231,722 + Rru_A0190 0.42 -0.5 231,723 - Rru_A0190 0.42 -1.1 231,807 - Rru_A0190 0.46 +0.3 231,821 + Rru_A0190 0.47 -1.9 231,821 + Rru_A0190 0.47 -1.3 231,821 + Rru_A0190 0.47 +0.7 231,822 - Rru_A0190 0.47 +0.9 231,950 + Rru_A0190 0.53 -0.6 232,006 + Rru_A0190 0.56 -3.5 232,055 + Rru_A0190 0.58 +0.3 232,085 + Rru_A0190 0.59 +0.2 232,085 + Rru_A0190 0.59 +0.0 232,086 - Rru_A0190 0.59 -0.4 232,133 + Rru_A0190 0.62 +0.3 232,241 + Rru_A0190 0.67 -0.2 232,241 + Rru_A0190 0.67 +1.1 232,241 + Rru_A0190 0.67 -2.1 232,241 + Rru_A0190 0.67 +0.2 232,242 - Rru_A0190 0.67 -1.6 232,263 + Rru_A0190 0.68 -3.7 232,264 - Rru_A0190 0.68 -2.0 232,264 - Rru_A0190 0.68 +1.2 232,436 + Rru_A0190 0.76 -0.1 232,436 + Rru_A0190 0.76 -1.3 232,487 + Rru_A0190 0.78 +1.1 232,488 - Rru_A0190 0.78 +0.1 232,488 - Rru_A0190 0.78 +0.6 232,488 - Rru_A0190 0.78 -0.2 232,490 + Rru_A0190 0.78 -1.9 232,490 + Rru_A0190 0.78 -0.9 232,491 - Rru_A0190 0.78 -0.1 232,491 - Rru_A0190 0.78 -3.2 232,491 - Rru_A0190 0.78 -2.9 232,491 - Rru_A0190 0.78 +0.9 232,491 - Rru_A0190 0.78 +0.9 232,491 - Rru_A0190 0.78 +0.1 232,532 + Rru_A0190 0.80 -1.8 232,532 + Rru_A0190 0.80 +0.5 232,532 + Rru_A0190 0.80 +0.3 232,532 + Rru_A0190 0.80 -2.5 232,532 + Rru_A0190 0.80 -0.4 232,533 - Rru_A0190 0.80 -3.9 232,533 - Rru_A0190 0.80 -0.2 232,533 - Rru_A0190 0.80 -0.0 233,022 + +0.1 233,069 + -0.7 233,070 - +2.0 233,081 + +0.3 233,081 + +0.6 233,081 + +0.5 233,082 - -0.4 233,082 - -1.4 233,082 - -0.1 233,082 - -1.8 233,082 - -0.5 233,082 - -3.3 233,082 - -3.3 233,082 - -0.5 233,115 - -3.9 233,517 + Rru_A0191 0.48 -0.3 233,517 + Rru_A0191 0.48 +0.6 233,517 + Rru_A0191 0.48 -0.0 233,517 + Rru_A0191 0.48 -0.6 233,517 + Rru_A0191 0.48 +0.5 233,517 + Rru_A0191 0.48 -2.3 233,517 + Rru_A0191 0.48 -0.7 233,518 - Rru_A0191 0.48 -0.6 233,518 - Rru_A0191 0.48 +0.6 233,518 - Rru_A0191 0.48 -2.2 233,518 - Rru_A0191 0.48 +0.4 233,518 - Rru_A0191 0.48 +1.0 233,518 - Rru_A0191 0.48 -0.7 233,518 - Rru_A0191 0.48 -2.8 233,518 - Rru_A0191 0.48 -1.5 233,635 + Rru_A0191 0.77 -2.2 233,635 + Rru_A0191 0.77 +0.4 233,635 + Rru_A0191 0.77 -0.1 233,636 - Rru_A0191 0.77 +0.8 233,636 - Rru_A0191 0.77 +1.1 233,681 - Rru_A0191 0.88 -0.9 233,681 - Rru_A0191 0.88 -0.8 233,681 - Rru_A0191 0.88 +0.9 233,750 + +1.2 233,780 - -2.5
Or see this region's nucleotide sequence