Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_B0038 and Rru_B0039 are separated by 122 nucleotides Rru_B0039 and Rru_B0040 are separated by 6 nucleotides
Rru_B0038: Rru_B0038 - dTDP-4-dehydrorhamnose 3,5-epimerase (NCBI), at 38,320 to 38,886
_B0038
Rru_B0039: Rru_B0039 - Type I secretion system ATPase, PrtD (NCBI), at 39,009 to 40,766
_B0039
Rru_B0040: Rru_B0040 - Type I secretion membrane fusion protein, HlyD (NCBI), at 40,773 to 42,251
_B0040
Position (kb)
39
40
41 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 38.009 kb on - strand at 38.009 kb on - strand at 38.034 kb on + strand at 38.035 kb on - strand at 38.035 kb on - strand at 38.035 kb on - strand at 38.036 kb on + strand at 38.036 kb on + strand at 38.036 kb on + strand at 38.037 kb on - strand at 38.037 kb on - strand at 38.037 kb on - strand at 38.037 kb on - strand at 38.054 kb on + strand at 38.054 kb on + strand at 38.054 kb on + strand at 38.054 kb on + strand at 38.054 kb on + strand at 38.054 kb on + strand at 38.055 kb on - strand at 38.058 kb on + strand at 38.058 kb on + strand at 38.058 kb on + strand at 38.059 kb on - strand at 38.059 kb on - strand at 38.163 kb on + strand at 38.163 kb on + strand at 38.178 kb on + strand at 38.231 kb on + strand at 38.251 kb on + strand at 38.251 kb on + strand at 38.252 kb on - strand at 38.252 kb on - strand at 38.721 kb on + strand, within Rru_B0038 at 38.879 kb on - strand at 38.879 kb on - strand at 38.879 kb on - strand at 38.879 kb on - strand at 38.885 kb on - strand at 38.909 kb on - strand at 38.937 kb on + strand at 38.937 kb on + strand at 38.937 kb on + strand at 38.938 kb on - strand at 38.938 kb on - strand at 38.938 kb on - strand at 38.986 kb on + strand at 39.122 kb on + strand at 39.153 kb on + strand at 39.153 kb on + strand at 39.154 kb on - strand at 39.168 kb on + strand at 39.169 kb on - strand at 39.329 kb on + strand, within Rru_B0039 at 39.329 kb on + strand, within Rru_B0039 at 39.329 kb on + strand, within Rru_B0039 at 39.330 kb on - strand, within Rru_B0039 at 39.330 kb on - strand, within Rru_B0039 at 39.330 kb on - strand, within Rru_B0039 at 39.372 kb on + strand, within Rru_B0039 at 39.372 kb on + strand, within Rru_B0039 at 39.372 kb on + strand, within Rru_B0039 at 39.373 kb on - strand, within Rru_B0039 at 39.373 kb on - strand, within Rru_B0039 at 39.375 kb on - strand, within Rru_B0039 at 39.375 kb on - strand, within Rru_B0039 at 39.375 kb on - strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.615 kb on + strand, within Rru_B0039 at 39.616 kb on - strand, within Rru_B0039 at 39.616 kb on - strand, within Rru_B0039 at 39.616 kb on - strand, within Rru_B0039 at 39.616 kb on - strand, within Rru_B0039 at 39.616 kb on - strand, within Rru_B0039 at 39.616 kb on - strand, within Rru_B0039 at 39.616 kb on - strand, within Rru_B0039 at 39.813 kb on + strand, within Rru_B0039 at 39.813 kb on + strand, within Rru_B0039 at 39.813 kb on + strand, within Rru_B0039 at 39.813 kb on + strand, within Rru_B0039 at 39.813 kb on + strand, within Rru_B0039 at 39.814 kb on - strand, within Rru_B0039 at 39.814 kb on - strand, within Rru_B0039 at 39.814 kb on - strand, within Rru_B0039 at 39.814 kb on - strand, within Rru_B0039 at 39.942 kb on + strand, within Rru_B0039 at 39.942 kb on + strand, within Rru_B0039 at 39.943 kb on - strand, within Rru_B0039 at 39.943 kb on - strand, within Rru_B0039 at 40.257 kb on + strand, within Rru_B0039 at 40.257 kb on + strand, within Rru_B0039 at 40.258 kb on - strand, within Rru_B0039 at 40.258 kb on - strand, within Rru_B0039 at 40.258 kb on - strand, within Rru_B0039 at 40.258 kb on - strand, within Rru_B0039 at 40.258 kb on - strand, within Rru_B0039 at 40.399 kb on - strand, within Rru_B0039 at 40.399 kb on - strand, within Rru_B0039 at 40.399 kb on - strand, within Rru_B0039 at 40.476 kb on + strand, within Rru_B0039 at 40.476 kb on + strand, within Rru_B0039 at 40.476 kb on + strand, within Rru_B0039 at 40.476 kb on + strand, within Rru_B0039 at 40.477 kb on - strand, within Rru_B0039 at 40.477 kb on - strand, within Rru_B0039 at 40.477 kb on - strand, within Rru_B0039 at 40.477 kb on - strand, within Rru_B0039 at 40.477 kb on - strand, within Rru_B0039 at 40.478 kb on + strand, within Rru_B0039 at 40.478 kb on + strand, within Rru_B0039 at 40.478 kb on + strand, within Rru_B0039 at 40.479 kb on - strand, within Rru_B0039 at 40.479 kb on - strand, within Rru_B0039 at 40.604 kb on + strand at 40.605 kb on - strand at 40.605 kb on - strand at 40.605 kb on - strand at 40.605 kb on - strand at 40.764 kb on + strand at 40.764 kb on + strand at 40.764 kb on + strand at 40.764 kb on + strand at 40.764 kb on + strand at 40.764 kb on + strand at 40.764 kb on + strand at 40.765 kb on - strand at 40.765 kb on - strand at 40.765 kb on - strand at 40.765 kb on - strand at 40.765 kb on - strand at 40.765 kb on - strand at 40.765 kb on - strand at 40.765 kb on - strand at 40.765 kb on - strand at 40.784 kb on + strand at 40.784 kb on + strand at 40.784 kb on + strand at 40.785 kb on - strand at 41.253 kb on + strand, within Rru_B0040 at 41.253 kb on + strand, within Rru_B0040 at 41.253 kb on + strand, within Rru_B0040 at 41.253 kb on + strand, within Rru_B0040 at 41.253 kb on + strand, within Rru_B0040 at 41.253 kb on + strand, within Rru_B0040 at 41.253 kb on + strand, within Rru_B0040 at 41.253 kb on + strand, within Rru_B0040 at 41.254 kb on - strand, within Rru_B0040 at 41.254 kb on - strand, within Rru_B0040 at 41.254 kb on - strand, within Rru_B0040 at 41.399 kb on + strand, within Rru_B0040 at 41.399 kb on + strand, within Rru_B0040 at 41.399 kb on + strand, within Rru_B0040 at 41.399 kb on + strand, within Rru_B0040 at 41.399 kb on + strand, within Rru_B0040 at 41.399 kb on + strand, within Rru_B0040 at 41.399 kb on + strand, within Rru_B0040 at 41.400 kb on - strand, within Rru_B0040 at 41.400 kb on - strand, within Rru_B0040 at 41.400 kb on - strand, within Rru_B0040 at 41.556 kb on + strand, within Rru_B0040 at 41.556 kb on + strand, within Rru_B0040 at 41.557 kb on - strand, within Rru_B0040 at 41.557 kb on - strand, within Rru_B0040 at 41.557 kb on - strand, within Rru_B0040 at 41.557 kb on - strand, within Rru_B0040 at 41.557 kb on - strand, within Rru_B0040 at 41.557 kb on - strand, within Rru_B0040 at 41.557 kb on - strand, within Rru_B0040 at 41.558 kb on + strand, within Rru_B0040 at 41.559 kb on - strand, within Rru_B0040 at 41.627 kb on + strand, within Rru_B0040 at 41.627 kb on + strand, within Rru_B0040 at 41.627 kb on + strand, within Rru_B0040 at 41.627 kb on + strand, within Rru_B0040 at 41.627 kb on + strand, within Rru_B0040 at 41.628 kb on - strand, within Rru_B0040 at 41.628 kb on - strand, within Rru_B0040
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 38,009 - +0.8 38,009 - -0.8 38,034 + -0.3 38,035 - -3.1 38,035 - -4.2 38,035 - -4.7 38,036 + -3.0 38,036 + +0.3 38,036 + -0.8 38,037 - +0.0 38,037 - +0.9 38,037 - -0.2 38,037 - -1.9 38,054 + -3.1 38,054 + -4.3 38,054 + +0.5 38,054 + +0.6 38,054 + +1.3 38,054 + +1.0 38,055 - -0.5 38,058 + -0.3 38,058 + +0.0 38,058 + +0.4 38,059 - -2.1 38,059 - -1.6 38,163 + -3.8 38,163 + +0.9 38,178 + -3.9 38,231 + +0.3 38,251 + +0.3 38,251 + -0.4 38,252 - +0.2 38,252 - -4.7 38,721 + Rru_B0038 0.71 -0.5 38,879 - -0.6 38,879 - +1.2 38,879 - -0.2 38,879 - -3.4 38,885 - -3.2 38,909 - -4.1 38,937 + +0.7 38,937 + -1.0 38,937 + +0.5 38,938 - +1.1 38,938 - +1.9 38,938 - +1.1 38,986 + -2.9 39,122 + +0.7 39,153 + +0.5 39,153 + +1.6 39,154 - +0.7 39,168 + -4.8 39,169 - -2.5 39,329 + Rru_B0039 0.18 -4.6 39,329 + Rru_B0039 0.18 -3.9 39,329 + Rru_B0039 0.18 +0.4 39,330 - Rru_B0039 0.18 -1.6 39,330 - Rru_B0039 0.18 -0.2 39,330 - Rru_B0039 0.18 +0.7 39,372 + Rru_B0039 0.21 -2.3 39,372 + Rru_B0039 0.21 -5.3 39,372 + Rru_B0039 0.21 -4.4 39,373 - Rru_B0039 0.21 +2.0 39,373 - Rru_B0039 0.21 -2.5 39,375 - Rru_B0039 0.21 +0.3 39,375 - Rru_B0039 0.21 -3.4 39,375 - Rru_B0039 0.21 -3.0 39,615 + Rru_B0039 0.34 +1.1 39,615 + Rru_B0039 0.34 -0.3 39,615 + Rru_B0039 0.34 +1.2 39,615 + Rru_B0039 0.34 +2.3 39,615 + Rru_B0039 0.34 -0.2 39,615 + Rru_B0039 0.34 +0.1 39,615 + Rru_B0039 0.34 +0.5 39,615 + Rru_B0039 0.34 +1.7 39,615 + Rru_B0039 0.34 +1.8 39,615 + Rru_B0039 0.34 +0.6 39,615 + Rru_B0039 0.34 -0.5 39,615 + Rru_B0039 0.34 -0.6 39,615 + Rru_B0039 0.34 +1.4 39,615 + Rru_B0039 0.34 +1.5 39,616 - Rru_B0039 0.35 -2.3 39,616 - Rru_B0039 0.35 -0.3 39,616 - Rru_B0039 0.35 -0.1 39,616 - Rru_B0039 0.35 -0.7 39,616 - Rru_B0039 0.35 +1.6 39,616 - Rru_B0039 0.35 +1.1 39,616 - Rru_B0039 0.35 +0.7 39,813 + Rru_B0039 0.46 +0.6 39,813 + Rru_B0039 0.46 -0.0 39,813 + Rru_B0039 0.46 -2.6 39,813 + Rru_B0039 0.46 -3.3 39,813 + Rru_B0039 0.46 -0.4 39,814 - Rru_B0039 0.46 -1.7 39,814 - Rru_B0039 0.46 +1.5 39,814 - Rru_B0039 0.46 +0.8 39,814 - Rru_B0039 0.46 -3.8 39,942 + Rru_B0039 0.53 -1.4 39,942 + Rru_B0039 0.53 -1.0 39,943 - Rru_B0039 0.53 +0.5 39,943 - Rru_B0039 0.53 +0.5 40,257 + Rru_B0039 0.71 +0.8 40,257 + Rru_B0039 0.71 -0.4 40,258 - Rru_B0039 0.71 -1.8 40,258 - Rru_B0039 0.71 +0.8 40,258 - Rru_B0039 0.71 +1.1 40,258 - Rru_B0039 0.71 -1.7 40,258 - Rru_B0039 0.71 -0.1 40,399 - Rru_B0039 0.79 +0.7 40,399 - Rru_B0039 0.79 -3.3 40,399 - Rru_B0039 0.79 +2.6 40,476 + Rru_B0039 0.83 +0.8 40,476 + Rru_B0039 0.83 -5.9 40,476 + Rru_B0039 0.83 -3.3 40,476 + Rru_B0039 0.83 +1.7 40,477 - Rru_B0039 0.84 +0.3 40,477 - Rru_B0039 0.84 +0.7 40,477 - Rru_B0039 0.84 -0.0 40,477 - Rru_B0039 0.84 +1.7 40,477 - Rru_B0039 0.84 -0.7 40,478 + Rru_B0039 0.84 +0.5 40,478 + Rru_B0039 0.84 +3.2 40,478 + Rru_B0039 0.84 -0.2 40,479 - Rru_B0039 0.84 +0.2 40,479 - Rru_B0039 0.84 -0.9 40,604 + +0.8 40,605 - -1.6 40,605 - +0.9 40,605 - +1.9 40,605 - +0.8 40,764 + -3.7 40,764 + -3.8 40,764 + -0.2 40,764 + -0.5 40,764 + +0.5 40,764 + -1.6 40,764 + -3.6 40,765 - -0.6 40,765 - +0.2 40,765 - +0.1 40,765 - -0.1 40,765 - -2.4 40,765 - +0.3 40,765 - -5.7 40,765 - +0.9 40,765 - -0.8 40,784 + -3.1 40,784 + -2.7 40,784 + +1.1 40,785 - +1.3 41,253 + Rru_B0040 0.32 -1.7 41,253 + Rru_B0040 0.32 -0.2 41,253 + Rru_B0040 0.32 -0.3 41,253 + Rru_B0040 0.32 +0.8 41,253 + Rru_B0040 0.32 +0.3 41,253 + Rru_B0040 0.32 +0.9 41,253 + Rru_B0040 0.32 -4.0 41,253 + Rru_B0040 0.32 +1.3 41,254 - Rru_B0040 0.33 +1.7 41,254 - Rru_B0040 0.33 +0.3 41,254 - Rru_B0040 0.33 +0.5 41,399 + Rru_B0040 0.42 +0.8 41,399 + Rru_B0040 0.42 -1.1 41,399 + Rru_B0040 0.42 +0.1 41,399 + Rru_B0040 0.42 +1.5 41,399 + Rru_B0040 0.42 -0.1 41,399 + Rru_B0040 0.42 +1.0 41,399 + Rru_B0040 0.42 -0.3 41,400 - Rru_B0040 0.42 -4.4 41,400 - Rru_B0040 0.42 +0.8 41,400 - Rru_B0040 0.42 -4.9 41,556 + Rru_B0040 0.53 -0.7 41,556 + Rru_B0040 0.53 -0.9 41,557 - Rru_B0040 0.53 -0.8 41,557 - Rru_B0040 0.53 -0.7 41,557 - Rru_B0040 0.53 -3.8 41,557 - Rru_B0040 0.53 -2.2 41,557 - Rru_B0040 0.53 +0.9 41,557 - Rru_B0040 0.53 +0.9 41,557 - Rru_B0040 0.53 +0.1 41,558 + Rru_B0040 0.53 -1.6 41,559 - Rru_B0040 0.53 +1.5 41,627 + Rru_B0040 0.58 -1.9 41,627 + Rru_B0040 0.58 +0.9 41,627 + Rru_B0040 0.58 +0.2 41,627 + Rru_B0040 0.58 -3.8 41,627 + Rru_B0040 0.58 +1.7 41,628 - Rru_B0040 0.58 +0.4 41,628 - Rru_B0040 0.58 +1.3
Or see this region's nucleotide sequence