Experiment: Gentamicin 0.00048828125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrH and frr overlap by 14 nucleotides frr and Synpcc7942_0508 are separated by 6 nucleotides Synpcc7942_0508 and Synpcc7942_0509 are separated by 38 nucleotides Synpcc7942_0509 and serB overlap by 4 nucleotides
Synpcc7942_0506: pyrH - uridylate kinase, at 492,988 to 493,749
pyrH
Synpcc7942_0507: frr - ribosome recycling factor, at 493,736 to 494,284
frr
Synpcc7942_0508: Synpcc7942_0508 - geranylgeranyl reductase, at 494,291 to 495,421
_0508
Synpcc7942_0509: Synpcc7942_0509 - hypothetical protein, at 495,460 to 495,933
_0509
Synpcc7942_0510: serB - 2-hydroxy-3-keto-5-methylthiopentenyl-1- phosphate phosphatase, at 495,930 to 496,580
serB
Position (kb) 494
495
496 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 494.286 kb on - strand at 494.286 kb on - strand at 494.299 kb on - strand at 494.318 kb on + strand at 494.354 kb on - strand at 494.369 kb on - strand at 494.390 kb on + strand at 494.394 kb on - strand at 494.402 kb on + strand at 494.402 kb on + strand at 494.415 kb on - strand, within Synpcc7942_0508 at 494.420 kb on - strand, within Synpcc7942_0508 at 494.468 kb on + strand, within Synpcc7942_0508 at 494.484 kb on - strand, within Synpcc7942_0508 at 494.556 kb on + strand, within Synpcc7942_0508 at 494.559 kb on - strand, within Synpcc7942_0508 at 494.559 kb on - strand, within Synpcc7942_0508 at 494.628 kb on - strand, within Synpcc7942_0508 at 494.646 kb on - strand, within Synpcc7942_0508 at 494.646 kb on - strand, within Synpcc7942_0508 at 494.674 kb on + strand, within Synpcc7942_0508 at 494.692 kb on + strand, within Synpcc7942_0508 at 494.697 kb on + strand, within Synpcc7942_0508 at 494.709 kb on + strand, within Synpcc7942_0508 at 494.738 kb on - strand, within Synpcc7942_0508 at 494.751 kb on + strand, within Synpcc7942_0508 at 494.753 kb on - strand, within Synpcc7942_0508 at 494.791 kb on - strand, within Synpcc7942_0508 at 494.804 kb on - strand, within Synpcc7942_0508 at 494.809 kb on - strand, within Synpcc7942_0508 at 494.824 kb on + strand, within Synpcc7942_0508 at 494.828 kb on - strand, within Synpcc7942_0508 at 494.832 kb on - strand, within Synpcc7942_0508 at 494.832 kb on - strand, within Synpcc7942_0508 at 494.837 kb on - strand, within Synpcc7942_0508 at 494.916 kb on - strand, within Synpcc7942_0508 at 494.916 kb on - strand, within Synpcc7942_0508 at 494.941 kb on + strand, within Synpcc7942_0508 at 494.981 kb on - strand, within Synpcc7942_0508 at 495.034 kb on + strand, within Synpcc7942_0508 at 495.041 kb on + strand, within Synpcc7942_0508 at 495.041 kb on + strand, within Synpcc7942_0508 at 495.041 kb on + strand, within Synpcc7942_0508 at 495.042 kb on - strand, within Synpcc7942_0508 at 495.042 kb on - strand, within Synpcc7942_0508 at 495.049 kb on - strand, within Synpcc7942_0508 at 495.049 kb on - strand, within Synpcc7942_0508 at 495.049 kb on - strand, within Synpcc7942_0508 at 495.083 kb on + strand, within Synpcc7942_0508 at 495.091 kb on - strand, within Synpcc7942_0508 at 495.095 kb on + strand, within Synpcc7942_0508 at 495.098 kb on - strand, within Synpcc7942_0508 at 495.102 kb on - strand, within Synpcc7942_0508 at 495.102 kb on - strand, within Synpcc7942_0508 at 495.111 kb on + strand, within Synpcc7942_0508 at 495.127 kb on + strand, within Synpcc7942_0508 at 495.127 kb on + strand, within Synpcc7942_0508 at 495.135 kb on - strand, within Synpcc7942_0508 at 495.194 kb on + strand at 495.194 kb on + strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.217 kb on + strand, within Synpcc7942_0508 at 495.217 kb on + strand, within Synpcc7942_0508 at 495.217 kb on - strand, within Synpcc7942_0508 at 495.229 kb on + strand, within Synpcc7942_0508 at 495.235 kb on + strand, within Synpcc7942_0508 at 495.235 kb on + strand, within Synpcc7942_0508 at 495.235 kb on + strand, within Synpcc7942_0508 at 495.235 kb on + strand, within Synpcc7942_0508 at 495.235 kb on + strand, within Synpcc7942_0508 at 495.236 kb on + strand, within Synpcc7942_0508 at 495.243 kb on - strand, within Synpcc7942_0508 at 495.243 kb on - strand at 495.243 kb on - strand, within Synpcc7942_0508 at 495.243 kb on - strand, within Synpcc7942_0508 at 495.243 kb on - strand, within Synpcc7942_0508 at 495.272 kb on + strand at 495.282 kb on - strand, within Synpcc7942_0508 at 495.304 kb on - strand, within Synpcc7942_0508 at 495.315 kb on - strand at 495.352 kb on + strand at 495.353 kb on + strand at 495.360 kb on - strand at 495.419 kb on - strand at 495.423 kb on - strand at 495.423 kb on - strand at 495.428 kb on + strand at 495.431 kb on - strand at 495.458 kb on + strand at 495.458 kb on + strand at 495.548 kb on - strand, within Synpcc7942_0509 at 495.555 kb on + strand, within Synpcc7942_0509 at 495.563 kb on - strand, within Synpcc7942_0509 at 495.575 kb on + strand, within Synpcc7942_0509 at 495.592 kb on - strand, within Synpcc7942_0509 at 495.656 kb on + strand, within Synpcc7942_0509 at 495.674 kb on - strand, within Synpcc7942_0509 at 495.680 kb on + strand, within Synpcc7942_0509 at 495.685 kb on + strand, within Synpcc7942_0509 at 495.710 kb on - strand, within Synpcc7942_0509 at 495.722 kb on - strand, within Synpcc7942_0509 at 495.731 kb on - strand, within Synpcc7942_0509 at 495.733 kb on + strand at 495.759 kb on + strand, within Synpcc7942_0509 at 495.766 kb on + strand, within Synpcc7942_0509 at 495.780 kb on - strand, within Synpcc7942_0509 at 495.791 kb on - strand, within Synpcc7942_0509 at 495.829 kb on - strand, within Synpcc7942_0509 at 495.850 kb on + strand, within Synpcc7942_0509 at 495.850 kb on + strand, within Synpcc7942_0509 at 495.858 kb on - strand, within Synpcc7942_0509 at 495.865 kb on + strand, within Synpcc7942_0509 at 495.896 kb on + strand at 495.903 kb on - strand at 495.943 kb on - strand at 495.956 kb on - strand at 495.979 kb on - strand at 495.979 kb on - strand at 495.981 kb on + strand at 496.119 kb on - strand, within serB at 496.148 kb on + strand, within serB at 496.159 kb on + strand, within serB at 496.171 kb on + strand, within serB at 496.174 kb on - strand, within serB at 496.176 kb on + strand, within serB at 496.212 kb on + strand, within serB at 496.213 kb on + strand, within serB at 496.223 kb on - strand, within serB at 496.232 kb on + strand, within serB at 496.250 kb on - strand, within serB at 496.255 kb on + strand, within serB at 496.263 kb on - strand, within serB at 496.264 kb on + strand, within serB at 496.267 kb on - strand, within serB at 496.346 kb on + strand, within serB at 496.351 kb on + strand at 496.351 kb on + strand, within serB at 496.354 kb on - strand, within serB at 496.354 kb on - strand, within serB at 496.354 kb on - strand, within serB at 496.354 kb on - strand, within serB at 496.359 kb on - strand, within serB
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.00048828125 mg/ml remove 494,286 - -1.6 494,286 - -0.0 494,299 - -0.3 494,318 + +2.5 494,354 - +1.6 494,369 - +1.9 494,390 + +1.8 494,394 - +1.9 494,402 + +1.5 494,402 + +0.4 494,415 - Synpcc7942_0508 0.11 +3.4 494,420 - Synpcc7942_0508 0.11 +1.8 494,468 + Synpcc7942_0508 0.16 +0.7 494,484 - Synpcc7942_0508 0.17 +1.0 494,556 + Synpcc7942_0508 0.23 +1.4 494,559 - Synpcc7942_0508 0.24 +1.8 494,559 - Synpcc7942_0508 0.24 +2.2 494,628 - Synpcc7942_0508 0.30 +3.5 494,646 - Synpcc7942_0508 0.31 +2.1 494,646 - Synpcc7942_0508 0.31 +2.5 494,674 + Synpcc7942_0508 0.34 +2.7 494,692 + Synpcc7942_0508 0.35 +1.0 494,697 + Synpcc7942_0508 0.36 +2.2 494,709 + Synpcc7942_0508 0.37 +1.5 494,738 - Synpcc7942_0508 0.40 -2.3 494,751 + Synpcc7942_0508 0.41 -0.4 494,753 - Synpcc7942_0508 0.41 +3.0 494,791 - Synpcc7942_0508 0.44 +2.6 494,804 - Synpcc7942_0508 0.45 +2.1 494,809 - Synpcc7942_0508 0.46 +1.9 494,824 + Synpcc7942_0508 0.47 -0.0 494,828 - Synpcc7942_0508 0.47 +1.7 494,832 - Synpcc7942_0508 0.48 +2.5 494,832 - Synpcc7942_0508 0.48 +2.4 494,837 - Synpcc7942_0508 0.48 +0.9 494,916 - Synpcc7942_0508 0.55 +4.0 494,916 - Synpcc7942_0508 0.55 +2.3 494,941 + Synpcc7942_0508 0.57 +3.0 494,981 - Synpcc7942_0508 0.61 +1.2 495,034 + Synpcc7942_0508 0.66 +1.5 495,041 + Synpcc7942_0508 0.66 +2.9 495,041 + Synpcc7942_0508 0.66 -1.6 495,041 + Synpcc7942_0508 0.66 +2.2 495,042 - Synpcc7942_0508 0.66 +1.8 495,042 - Synpcc7942_0508 0.66 +3.8 495,049 - Synpcc7942_0508 0.67 +2.0 495,049 - Synpcc7942_0508 0.67 +3.2 495,049 - Synpcc7942_0508 0.67 +1.9 495,083 + Synpcc7942_0508 0.70 +3.2 495,091 - Synpcc7942_0508 0.71 +1.6 495,095 + Synpcc7942_0508 0.71 +1.8 495,098 - Synpcc7942_0508 0.71 +2.6 495,102 - Synpcc7942_0508 0.72 +1.4 495,102 - Synpcc7942_0508 0.72 +1.6 495,111 + Synpcc7942_0508 0.73 +2.3 495,127 + Synpcc7942_0508 0.74 +3.9 495,127 + Synpcc7942_0508 0.74 +1.8 495,135 - Synpcc7942_0508 0.75 +1.2 495,194 + +2.1 495,194 + Synpcc7942_0508 0.80 +3.2 495,202 - Synpcc7942_0508 0.81 +2.7 495,202 - Synpcc7942_0508 0.81 +3.7 495,202 - Synpcc7942_0508 0.81 +2.2 495,202 - Synpcc7942_0508 0.81 +1.9 495,202 - Synpcc7942_0508 0.81 +1.6 495,202 - Synpcc7942_0508 0.81 +2.9 495,202 - Synpcc7942_0508 0.81 +0.6 495,217 + Synpcc7942_0508 0.82 +3.9 495,217 + Synpcc7942_0508 0.82 -0.5 495,217 - Synpcc7942_0508 0.82 +2.2 495,229 + Synpcc7942_0508 0.83 +0.7 495,235 + Synpcc7942_0508 0.83 +1.8 495,235 + Synpcc7942_0508 0.83 +0.5 495,235 + Synpcc7942_0508 0.83 +2.6 495,235 + Synpcc7942_0508 0.83 +2.6 495,235 + Synpcc7942_0508 0.83 +2.6 495,236 + Synpcc7942_0508 0.84 +3.6 495,243 - Synpcc7942_0508 0.84 +2.7 495,243 - -3.2 495,243 - Synpcc7942_0508 0.84 +1.6 495,243 - Synpcc7942_0508 0.84 +2.3 495,243 - Synpcc7942_0508 0.84 +2.8 495,272 + -0.0 495,282 - Synpcc7942_0508 0.88 +0.8 495,304 - Synpcc7942_0508 0.90 +2.8 495,315 - -0.4 495,352 + +2.4 495,353 + +2.9 495,360 - -0.1 495,419 - +1.5 495,423 - -0.2 495,423 - +0.8 495,428 + -1.0 495,431 - -0.9 495,458 + -1.6 495,458 + -1.6 495,548 - Synpcc7942_0509 0.19 -1.4 495,555 + Synpcc7942_0509 0.20 -0.4 495,563 - Synpcc7942_0509 0.22 -2.2 495,575 + Synpcc7942_0509 0.24 -0.2 495,592 - Synpcc7942_0509 0.28 +0.8 495,656 + Synpcc7942_0509 0.41 +1.0 495,674 - Synpcc7942_0509 0.45 +1.7 495,680 + Synpcc7942_0509 0.46 +0.4 495,685 + Synpcc7942_0509 0.47 +1.0 495,710 - Synpcc7942_0509 0.53 -1.0 495,722 - Synpcc7942_0509 0.55 -0.1 495,731 - Synpcc7942_0509 0.57 +0.8 495,733 + -1.6 495,759 + Synpcc7942_0509 0.63 +1.8 495,766 + Synpcc7942_0509 0.65 +1.0 495,780 - Synpcc7942_0509 0.68 +1.0 495,791 - Synpcc7942_0509 0.70 +0.6 495,829 - Synpcc7942_0509 0.78 +0.4 495,850 + Synpcc7942_0509 0.82 +2.3 495,850 + Synpcc7942_0509 0.82 +1.5 495,858 - Synpcc7942_0509 0.84 +1.2 495,865 + Synpcc7942_0509 0.85 +0.9 495,896 + +0.7 495,903 - +1.7 495,943 - +0.2 495,956 - -0.1 495,979 - -0.6 495,979 - +1.5 495,981 + +2.6 496,119 - serB Synpcc7942_0510 0.29 -0.5 496,148 + serB Synpcc7942_0510 0.33 +0.7 496,159 + serB Synpcc7942_0510 0.35 +2.2 496,171 + serB Synpcc7942_0510 0.37 +3.3 496,174 - serB Synpcc7942_0510 0.37 +0.1 496,176 + serB Synpcc7942_0510 0.38 +1.4 496,212 + serB Synpcc7942_0510 0.43 -0.5 496,213 + serB Synpcc7942_0510 0.43 +1.3 496,223 - serB Synpcc7942_0510 0.45 +0.4 496,232 + serB Synpcc7942_0510 0.46 +0.9 496,250 - serB Synpcc7942_0510 0.49 +0.8 496,255 + serB Synpcc7942_0510 0.50 +0.7 496,263 - serB Synpcc7942_0510 0.51 +0.3 496,264 + serB Synpcc7942_0510 0.51 +1.3 496,267 - serB Synpcc7942_0510 0.52 -0.8 496,346 + serB Synpcc7942_0510 0.64 +0.4 496,351 + -0.6 496,351 + serB Synpcc7942_0510 0.65 -1.6 496,354 - serB Synpcc7942_0510 0.65 -1.6 496,354 - serB Synpcc7942_0510 0.65 +2.6 496,354 - serB Synpcc7942_0510 0.65 +0.2 496,354 - serB Synpcc7942_0510 0.65 +1.4 496,359 - serB Synpcc7942_0510 0.66 +1.2
Or see this region's nucleotide sequence