Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28790 and RR42_RS28795 are separated by 346 nucleotides RR42_RS28795 and RR42_RS28800 are separated by 286 nucleotides RR42_RS28800 and RR42_RS28805 are separated by 118 nucleotides
RR42_RS28790: RR42_RS28790 - hypothetical protein, at 1,810,699 to 1,811,301
_RS28790
RR42_RS28795: RR42_RS28795 - hypothetical protein, at 1,811,648 to 1,811,866
_RS28795
RR42_RS28800: RR42_RS28800 - isocitrate dehydrogenase, at 1,812,153 to 1,814,390
_RS28800
RR42_RS28805: RR42_RS28805 - hypothetical protein, at 1,814,509 to 1,815,510
_RS28805
Position (kb)
1812
1813
1814
1815 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1811.355 kb on + strand at 1811.355 kb on + strand at 1811.356 kb on - strand at 1811.574 kb on - strand at 1811.591 kb on - strand at 1811.597 kb on + strand at 1811.597 kb on + strand at 1811.597 kb on + strand at 1811.597 kb on + strand at 1811.598 kb on - strand at 1811.598 kb on - strand at 1811.598 kb on - strand at 1811.601 kb on - strand at 1811.601 kb on - strand at 1811.611 kb on - strand at 1811.701 kb on - strand, within RR42_RS28795 at 1811.701 kb on - strand, within RR42_RS28795 at 1811.724 kb on + strand, within RR42_RS28795 at 1811.725 kb on - strand, within RR42_RS28795 at 1811.735 kb on - strand, within RR42_RS28795 at 1811.735 kb on - strand, within RR42_RS28795 at 1811.765 kb on + strand, within RR42_RS28795 at 1811.766 kb on - strand, within RR42_RS28795 at 1811.766 kb on - strand, within RR42_RS28795 at 1811.766 kb on - strand, within RR42_RS28795 at 1811.779 kb on - strand, within RR42_RS28795 at 1811.779 kb on - strand, within RR42_RS28795 at 1811.779 kb on - strand, within RR42_RS28795 at 1811.864 kb on + strand at 1811.865 kb on - strand at 1811.907 kb on + strand at 1812.129 kb on + strand at 1812.129 kb on + strand at 1812.130 kb on - strand at 1812.148 kb on - strand at 1812.180 kb on + strand at 1812.181 kb on - strand at 1812.300 kb on - strand at 1812.300 kb on - strand at 1812.300 kb on - strand at 1812.300 kb on - strand at 1812.471 kb on + strand, within RR42_RS28800 at 1812.534 kb on + strand, within RR42_RS28800 at 1812.618 kb on + strand, within RR42_RS28800 at 1812.619 kb on - strand, within RR42_RS28800 at 1812.702 kb on + strand, within RR42_RS28800 at 1812.703 kb on - strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.864 kb on + strand, within RR42_RS28800 at 1812.865 kb on - strand, within RR42_RS28800 at 1813.059 kb on + strand, within RR42_RS28800 at 1813.060 kb on - strand, within RR42_RS28800 at 1813.060 kb on - strand, within RR42_RS28800 at 1813.060 kb on - strand, within RR42_RS28800 at 1813.416 kb on + strand, within RR42_RS28800 at 1813.417 kb on - strand, within RR42_RS28800 at 1813.417 kb on - strand, within RR42_RS28800 at 1813.417 kb on - strand, within RR42_RS28800 at 1813.675 kb on - strand, within RR42_RS28800 at 1813.675 kb on - strand, within RR42_RS28800 at 1813.675 kb on - strand, within RR42_RS28800 at 1813.806 kb on + strand, within RR42_RS28800 at 1813.893 kb on + strand, within RR42_RS28800 at 1813.893 kb on + strand, within RR42_RS28800 at 1813.894 kb on - strand, within RR42_RS28800 at 1813.894 kb on - strand, within RR42_RS28800 at 1813.894 kb on - strand, within RR42_RS28800 at 1814.145 kb on + strand, within RR42_RS28800 at 1814.157 kb on + strand, within RR42_RS28800 at 1814.157 kb on + strand, within RR42_RS28800 at 1814.307 kb on + strand at 1814.307 kb on + strand at 1814.307 kb on + strand at 1814.307 kb on + strand at 1814.307 kb on + strand at 1814.307 kb on + strand at 1814.308 kb on - strand at 1814.308 kb on - strand at 1814.388 kb on + strand at 1814.495 kb on - strand at 1814.549 kb on + strand at 1814.557 kb on + strand at 1814.557 kb on + strand at 1814.557 kb on + strand at 1814.846 kb on + strand, within RR42_RS28805 at 1814.931 kb on - strand, within RR42_RS28805 at 1814.931 kb on - strand, within RR42_RS28805 at 1815.017 kb on + strand, within RR42_RS28805 at 1815.017 kb on + strand, within RR42_RS28805 at 1815.104 kb on - strand, within RR42_RS28805 at 1815.104 kb on - strand, within RR42_RS28805 at 1815.221 kb on + strand, within RR42_RS28805 at 1815.221 kb on + strand, within RR42_RS28805 at 1815.222 kb on - strand, within RR42_RS28805 at 1815.222 kb on - strand, within RR42_RS28805 at 1815.293 kb on - strand, within RR42_RS28805 at 1815.324 kb on + strand, within RR42_RS28805
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,811,355 + +0.2 1,811,355 + -0.0 1,811,356 - +0.8 1,811,574 - -0.7 1,811,591 - -2.5 1,811,597 + -0.2 1,811,597 + +0.7 1,811,597 + -0.9 1,811,597 + +0.1 1,811,598 - -0.7 1,811,598 - +1.0 1,811,598 - -0.2 1,811,601 - +0.7 1,811,601 - +0.1 1,811,611 - -0.6 1,811,701 - RR42_RS28795 0.24 -0.9 1,811,701 - RR42_RS28795 0.24 -0.7 1,811,724 + RR42_RS28795 0.35 -0.6 1,811,725 - RR42_RS28795 0.35 -1.1 1,811,735 - RR42_RS28795 0.40 +1.3 1,811,735 - RR42_RS28795 0.40 -0.9 1,811,765 + RR42_RS28795 0.53 +0.2 1,811,766 - RR42_RS28795 0.54 -0.3 1,811,766 - RR42_RS28795 0.54 +0.5 1,811,766 - RR42_RS28795 0.54 +1.6 1,811,779 - RR42_RS28795 0.60 -0.5 1,811,779 - RR42_RS28795 0.60 -1.5 1,811,779 - RR42_RS28795 0.60 -0.3 1,811,864 + -0.7 1,811,865 - +0.2 1,811,907 + -0.1 1,812,129 + +1.2 1,812,129 + -0.9 1,812,130 - +0.1 1,812,148 - -2.6 1,812,180 + -0.3 1,812,181 - -0.6 1,812,300 - -1.8 1,812,300 - -1.6 1,812,300 - -0.2 1,812,300 - -1.3 1,812,471 + RR42_RS28800 0.14 -0.0 1,812,534 + RR42_RS28800 0.17 -1.1 1,812,618 + RR42_RS28800 0.21 +0.3 1,812,619 - RR42_RS28800 0.21 -0.8 1,812,702 + RR42_RS28800 0.25 -0.7 1,812,703 - RR42_RS28800 0.25 +2.0 1,812,864 + RR42_RS28800 0.32 +1.3 1,812,864 + RR42_RS28800 0.32 -1.1 1,812,864 + RR42_RS28800 0.32 +0.4 1,812,864 + RR42_RS28800 0.32 -1.4 1,812,864 + RR42_RS28800 0.32 +1.0 1,812,864 + RR42_RS28800 0.32 -1.4 1,812,865 - RR42_RS28800 0.32 -1.6 1,813,059 + RR42_RS28800 0.40 -1.6 1,813,060 - RR42_RS28800 0.41 -0.2 1,813,060 - RR42_RS28800 0.41 -1.5 1,813,060 - RR42_RS28800 0.41 -0.4 1,813,416 + RR42_RS28800 0.56 +1.4 1,813,417 - RR42_RS28800 0.56 -1.7 1,813,417 - RR42_RS28800 0.56 +1.6 1,813,417 - RR42_RS28800 0.56 -0.7 1,813,675 - RR42_RS28800 0.68 -1.2 1,813,675 - RR42_RS28800 0.68 -1.8 1,813,675 - RR42_RS28800 0.68 -0.5 1,813,806 + RR42_RS28800 0.74 -0.1 1,813,893 + RR42_RS28800 0.78 -1.1 1,813,893 + RR42_RS28800 0.78 -1.1 1,813,894 - RR42_RS28800 0.78 +0.2 1,813,894 - RR42_RS28800 0.78 +1.0 1,813,894 - RR42_RS28800 0.78 -0.0 1,814,145 + RR42_RS28800 0.89 +0.4 1,814,157 + RR42_RS28800 0.90 -1.3 1,814,157 + RR42_RS28800 0.90 -0.7 1,814,307 + -1.1 1,814,307 + -1.0 1,814,307 + +1.6 1,814,307 + +0.2 1,814,307 + +0.6 1,814,307 + +0.4 1,814,308 - -1.2 1,814,308 - -0.8 1,814,388 + -0.4 1,814,495 - -0.3 1,814,549 + -1.6 1,814,557 + +0.1 1,814,557 + +0.7 1,814,557 + -0.6 1,814,846 + RR42_RS28805 0.34 -0.4 1,814,931 - RR42_RS28805 0.42 +0.1 1,814,931 - RR42_RS28805 0.42 +2.0 1,815,017 + RR42_RS28805 0.51 -0.4 1,815,017 + RR42_RS28805 0.51 +0.1 1,815,104 - RR42_RS28805 0.59 +1.2 1,815,104 - RR42_RS28805 0.59 -0.7 1,815,221 + RR42_RS28805 0.71 -2.0 1,815,221 + RR42_RS28805 0.71 -0.0 1,815,222 - RR42_RS28805 0.71 +0.2 1,815,222 - RR42_RS28805 0.71 -0.1 1,815,293 - RR42_RS28805 0.78 +0.6 1,815,324 + RR42_RS28805 0.81 -0.3
Or see this region's nucleotide sequence