Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25890 and RR42_RS25895 are separated by 148 nucleotides RR42_RS25895 and RR42_RS25900 are separated by 268 nucleotides
RR42_RS25890: RR42_RS25890 - Branched-chain amino acid aminotransferase (EC 2.6.1.42) (from data) , at 1,149,447 to 1,150,538
_RS25890
RR42_RS25895: RR42_RS25895 - IclR family transcriptional regulator, at 1,150,687 to 1,151,460
_RS25895
RR42_RS25900: RR42_RS25900 - enoyl-CoA hydratase, at 1,151,729 to 1,152,505
_RS25900
Position (kb)
1150
1151
1152 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1149.691 kb on - strand, within RR42_RS25890 at 1149.867 kb on + strand, within RR42_RS25890 at 1149.867 kb on + strand, within RR42_RS25890 at 1149.867 kb on + strand, within RR42_RS25890 at 1150.011 kb on + strand, within RR42_RS25890 at 1150.011 kb on + strand, within RR42_RS25890 at 1150.011 kb on + strand, within RR42_RS25890 at 1150.012 kb on - strand, within RR42_RS25890 at 1150.062 kb on + strand, within RR42_RS25890 at 1150.063 kb on - strand, within RR42_RS25890 at 1150.218 kb on - strand, within RR42_RS25890 at 1150.218 kb on - strand, within RR42_RS25890 at 1150.391 kb on - strand, within RR42_RS25890 at 1150.537 kb on - strand at 1150.570 kb on + strand at 1150.570 kb on + strand at 1150.570 kb on + strand at 1150.570 kb on + strand at 1150.570 kb on + strand at 1150.571 kb on - strand at 1150.571 kb on - strand at 1150.764 kb on - strand at 1150.884 kb on - strand, within RR42_RS25895 at 1150.898 kb on + strand, within RR42_RS25895 at 1150.898 kb on + strand, within RR42_RS25895 at 1151.206 kb on + strand, within RR42_RS25895 at 1151.300 kb on + strand, within RR42_RS25895 at 1151.301 kb on - strand, within RR42_RS25895 at 1151.663 kb on - strand at 1151.668 kb on - strand at 1151.692 kb on + strand at 1151.692 kb on + strand at 1151.693 kb on - strand at 1151.794 kb on - strand at 1151.794 kb on - strand at 1151.875 kb on + strand, within RR42_RS25900 at 1151.875 kb on + strand, within RR42_RS25900 at 1151.875 kb on + strand, within RR42_RS25900 at 1151.875 kb on + strand, within RR42_RS25900 at 1151.876 kb on - strand, within RR42_RS25900 at 1151.876 kb on - strand, within RR42_RS25900 at 1151.981 kb on + strand, within RR42_RS25900 at 1151.981 kb on + strand, within RR42_RS25900 at 1151.981 kb on + strand, within RR42_RS25900 at 1151.981 kb on + strand, within RR42_RS25900 at 1152.002 kb on + strand, within RR42_RS25900 at 1152.003 kb on - strand, within RR42_RS25900 at 1152.209 kb on + strand, within RR42_RS25900
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,149,691 - RR42_RS25890 0.22 -0.1 1,149,867 + RR42_RS25890 0.38 -1.7 1,149,867 + RR42_RS25890 0.38 -0.9 1,149,867 + RR42_RS25890 0.38 -0.1 1,150,011 + RR42_RS25890 0.52 -0.3 1,150,011 + RR42_RS25890 0.52 +0.7 1,150,011 + RR42_RS25890 0.52 +0.1 1,150,012 - RR42_RS25890 0.52 +0.5 1,150,062 + RR42_RS25890 0.56 -3.0 1,150,063 - RR42_RS25890 0.56 +0.1 1,150,218 - RR42_RS25890 0.71 -1.4 1,150,218 - RR42_RS25890 0.71 +0.7 1,150,391 - RR42_RS25890 0.86 -0.3 1,150,537 - -1.8 1,150,570 + +0.2 1,150,570 + +0.3 1,150,570 + +0.1 1,150,570 + -0.1 1,150,570 + +0.8 1,150,571 - -0.3 1,150,571 - +0.3 1,150,764 - -0.4 1,150,884 - RR42_RS25895 0.25 -2.2 1,150,898 + RR42_RS25895 0.27 +0.5 1,150,898 + RR42_RS25895 0.27 -0.4 1,151,206 + RR42_RS25895 0.67 +0.6 1,151,300 + RR42_RS25895 0.79 -0.7 1,151,301 - RR42_RS25895 0.79 -2.1 1,151,663 - -1.3 1,151,668 - +0.2 1,151,692 + -1.4 1,151,692 + -0.9 1,151,693 - -1.0 1,151,794 - +0.3 1,151,794 - +2.5 1,151,875 + RR42_RS25900 0.19 +0.1 1,151,875 + RR42_RS25900 0.19 +0.7 1,151,875 + RR42_RS25900 0.19 -0.7 1,151,875 + RR42_RS25900 0.19 -1.2 1,151,876 - RR42_RS25900 0.19 +1.5 1,151,876 - RR42_RS25900 0.19 -0.3 1,151,981 + RR42_RS25900 0.32 -0.9 1,151,981 + RR42_RS25900 0.32 -1.9 1,151,981 + RR42_RS25900 0.32 -0.7 1,151,981 + RR42_RS25900 0.32 -0.4 1,152,002 + RR42_RS25900 0.35 -0.7 1,152,003 - RR42_RS25900 0.35 -0.7 1,152,209 + RR42_RS25900 0.62 -0.3
Or see this region's nucleotide sequence