Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21975 and RR42_RS21980 are separated by 59 nucleotides RR42_RS21980 and RR42_RS21985 are separated by 13 nucleotides RR42_RS21985 and RR42_RS21990 overlap by 8 nucleotides RR42_RS21990 and RR42_RS21995 overlap by 4 nucleotides
RR42_RS21975: RR42_RS21975 - hypothetical protein, at 258,302 to 262,522
_RS21975
RR42_RS21980: RR42_RS21980 - transporter, at 262,582 to 263,139
_RS21980
RR42_RS21985: RR42_RS21985 - sugar ABC transporter, at 263,153 to 264,598
_RS21985
RR42_RS21990: RR42_RS21990 - LPS N-acetylglucosaminyl transferase, at 264,591 to 265,577
_RS21990
RR42_RS21995: RR42_RS21995 - glycosyl transferase family 1, at 265,574 to 267,175
_RS21995
Position (kb) 263
264
265 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 262.176 kb on - strand at 262.253 kb on + strand at 262.253 kb on + strand at 262.254 kb on - strand at 262.254 kb on - strand at 262.320 kb on - strand at 262.320 kb on - strand at 262.320 kb on - strand at 262.442 kb on + strand at 262.442 kb on + strand at 262.442 kb on + strand at 262.443 kb on - strand at 262.443 kb on - strand at 262.556 kb on + strand at 262.557 kb on - strand at 262.593 kb on + strand at 262.593 kb on + strand at 262.593 kb on + strand at 262.744 kb on + strand, within RR42_RS21980 at 262.744 kb on + strand, within RR42_RS21980 at 262.745 kb on - strand, within RR42_RS21980 at 262.961 kb on - strand, within RR42_RS21980 at 263.168 kb on - strand at 263.304 kb on - strand, within RR42_RS21985 at 263.978 kb on + strand, within RR42_RS21985 at 263.978 kb on + strand, within RR42_RS21985 at 264.066 kb on - strand, within RR42_RS21985 at 264.104 kb on + strand, within RR42_RS21985 at 264.398 kb on + strand, within RR42_RS21985 at 264.472 kb on + strand at 264.472 kb on + strand at 264.472 kb on + strand at 264.577 kb on - strand at 264.597 kb on - strand at 264.777 kb on + strand, within RR42_RS21990 at 264.778 kb on - strand, within RR42_RS21990 at 264.778 kb on - strand, within RR42_RS21990 at 264.778 kb on - strand, within RR42_RS21990 at 264.783 kb on + strand, within RR42_RS21990 at 264.793 kb on + strand, within RR42_RS21990 at 264.793 kb on + strand, within RR42_RS21990 at 264.793 kb on + strand, within RR42_RS21990 at 265.107 kb on + strand, within RR42_RS21990 at 265.245 kb on + strand, within RR42_RS21990 at 265.246 kb on - strand, within RR42_RS21990 at 265.273 kb on - strand, within RR42_RS21990 at 265.350 kb on + strand, within RR42_RS21990 at 265.351 kb on - strand, within RR42_RS21990 at 265.351 kb on - strand, within RR42_RS21990 at 265.351 kb on - strand, within RR42_RS21990 at 265.563 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 262,176 - +0.4 262,253 + -0.0 262,253 + +0.8 262,254 - -1.4 262,254 - -0.2 262,320 - +0.8 262,320 - -1.3 262,320 - -1.5 262,442 + -1.7 262,442 + -0.8 262,442 + +0.7 262,443 - +0.3 262,443 - -0.6 262,556 + +0.1 262,557 - -0.5 262,593 + +0.8 262,593 + +1.0 262,593 + -0.4 262,744 + RR42_RS21980 0.29 -0.4 262,744 + RR42_RS21980 0.29 -0.8 262,745 - RR42_RS21980 0.29 +0.4 262,961 - RR42_RS21980 0.68 -0.2 263,168 - -1.3 263,304 - RR42_RS21985 0.10 +0.3 263,978 + RR42_RS21985 0.57 +1.2 263,978 + RR42_RS21985 0.57 -3.2 264,066 - RR42_RS21985 0.63 +0.3 264,104 + RR42_RS21985 0.66 -0.5 264,398 + RR42_RS21985 0.86 -1.1 264,472 + -0.6 264,472 + +0.1 264,472 + -1.8 264,577 - -0.6 264,597 - +0.8 264,777 + RR42_RS21990 0.19 +1.7 264,778 - RR42_RS21990 0.19 -2.0 264,778 - RR42_RS21990 0.19 -0.8 264,778 - RR42_RS21990 0.19 -3.3 264,783 + RR42_RS21990 0.19 +0.6 264,793 + RR42_RS21990 0.20 -0.3 264,793 + RR42_RS21990 0.20 -0.3 264,793 + RR42_RS21990 0.20 -0.4 265,107 + RR42_RS21990 0.52 +0.2 265,245 + RR42_RS21990 0.66 -1.5 265,246 - RR42_RS21990 0.66 +1.0 265,273 - RR42_RS21990 0.69 +2.3 265,350 + RR42_RS21990 0.77 +0.4 265,351 - RR42_RS21990 0.77 +0.4 265,351 - RR42_RS21990 0.77 -0.8 265,351 - RR42_RS21990 0.77 -2.2 265,563 + +1.9
Or see this region's nucleotide sequence