Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21570 and RR42_RS21575 are separated by 125 nucleotides RR42_RS21575 and RR42_RS21580 are separated by 34 nucleotides
RR42_RS21570: RR42_RS21570 - membrane protein, at 164,956 to 166,104
_RS21570
RR42_RS21575: RR42_RS21575 - peptidase E, at 166,230 to 166,985
_RS21575
RR42_RS21580: RR42_RS21580 - cysteine desulfurase, at 167,020 to 168,204
_RS21580
Position (kb)
166
167 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 165.236 kb on + strand, within RR42_RS21570 at 165.237 kb on - strand, within RR42_RS21570 at 165.237 kb on - strand, within RR42_RS21570 at 165.237 kb on - strand, within RR42_RS21570 at 165.237 kb on - strand, within RR42_RS21570 at 165.237 kb on - strand, within RR42_RS21570 at 165.400 kb on - strand, within RR42_RS21570 at 165.400 kb on - strand, within RR42_RS21570 at 165.401 kb on + strand, within RR42_RS21570 at 165.401 kb on + strand, within RR42_RS21570 at 165.402 kb on - strand, within RR42_RS21570 at 165.402 kb on - strand, within RR42_RS21570 at 165.402 kb on - strand, within RR42_RS21570 at 165.402 kb on - strand, within RR42_RS21570 at 165.452 kb on + strand, within RR42_RS21570 at 165.527 kb on + strand, within RR42_RS21570 at 165.527 kb on + strand, within RR42_RS21570 at 165.617 kb on + strand, within RR42_RS21570 at 165.618 kb on - strand, within RR42_RS21570 at 165.630 kb on - strand, within RR42_RS21570 at 165.648 kb on - strand, within RR42_RS21570 at 165.716 kb on + strand, within RR42_RS21570 at 165.717 kb on - strand, within RR42_RS21570 at 165.717 kb on - strand, within RR42_RS21570 at 165.717 kb on - strand, within RR42_RS21570 at 165.717 kb on - strand, within RR42_RS21570 at 165.717 kb on - strand, within RR42_RS21570 at 165.719 kb on + strand, within RR42_RS21570 at 165.719 kb on + strand, within RR42_RS21570 at 165.720 kb on - strand, within RR42_RS21570 at 165.744 kb on - strand, within RR42_RS21570 at 165.744 kb on - strand, within RR42_RS21570 at 165.795 kb on - strand, within RR42_RS21570 at 165.795 kb on - strand, within RR42_RS21570 at 165.795 kb on - strand, within RR42_RS21570 at 165.795 kb on - strand, within RR42_RS21570 at 165.961 kb on - strand, within RR42_RS21570 at 165.999 kb on - strand at 166.128 kb on - strand at 166.128 kb on - strand at 166.128 kb on - strand at 166.318 kb on - strand, within RR42_RS21575 at 166.631 kb on - strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.831 kb on + strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.832 kb on - strand, within RR42_RS21575 at 166.934 kb on - strand at 166.998 kb on + strand at 167.073 kb on + strand at 167.074 kb on - strand at 167.074 kb on - strand at 167.081 kb on + strand at 167.082 kb on - strand at 167.117 kb on + strand at 167.264 kb on + strand, within RR42_RS21580 at 167.468 kb on + strand, within RR42_RS21580 at 167.468 kb on + strand, within RR42_RS21580 at 167.469 kb on - strand, within RR42_RS21580 at 167.469 kb on - strand, within RR42_RS21580 at 167.531 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.943 kb on + strand, within RR42_RS21580 at 167.944 kb on - strand, within RR42_RS21580 at 167.944 kb on - strand, within RR42_RS21580
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 165,236 + RR42_RS21570 0.24 +0.5 165,237 - RR42_RS21570 0.24 -1.2 165,237 - RR42_RS21570 0.24 +0.4 165,237 - RR42_RS21570 0.24 -0.2 165,237 - RR42_RS21570 0.24 -0.7 165,237 - RR42_RS21570 0.24 +1.4 165,400 - RR42_RS21570 0.39 -0.4 165,400 - RR42_RS21570 0.39 -0.2 165,401 + RR42_RS21570 0.39 -0.4 165,401 + RR42_RS21570 0.39 -0.6 165,402 - RR42_RS21570 0.39 -1.1 165,402 - RR42_RS21570 0.39 -0.2 165,402 - RR42_RS21570 0.39 -1.1 165,402 - RR42_RS21570 0.39 +1.4 165,452 + RR42_RS21570 0.43 +0.8 165,527 + RR42_RS21570 0.50 +0.2 165,527 + RR42_RS21570 0.50 -0.6 165,617 + RR42_RS21570 0.58 +0.3 165,618 - RR42_RS21570 0.58 -1.2 165,630 - RR42_RS21570 0.59 -0.2 165,648 - RR42_RS21570 0.60 -0.2 165,716 + RR42_RS21570 0.66 +0.1 165,717 - RR42_RS21570 0.66 -1.1 165,717 - RR42_RS21570 0.66 -0.2 165,717 - RR42_RS21570 0.66 -1.2 165,717 - RR42_RS21570 0.66 +0.0 165,717 - RR42_RS21570 0.66 -0.7 165,719 + RR42_RS21570 0.66 +0.0 165,719 + RR42_RS21570 0.66 -2.0 165,720 - RR42_RS21570 0.66 +0.3 165,744 - RR42_RS21570 0.69 -0.2 165,744 - RR42_RS21570 0.69 +1.4 165,795 - RR42_RS21570 0.73 -0.4 165,795 - RR42_RS21570 0.73 -0.8 165,795 - RR42_RS21570 0.73 -0.2 165,795 - RR42_RS21570 0.73 -0.6 165,961 - RR42_RS21570 0.87 +0.8 165,999 - -1.1 166,128 - -0.4 166,128 - -1.0 166,128 - -1.0 166,318 - RR42_RS21575 0.12 -0.8 166,631 - RR42_RS21575 0.53 -0.6 166,831 + RR42_RS21575 0.79 -0.2 166,831 + RR42_RS21575 0.79 -0.4 166,831 + RR42_RS21575 0.79 -0.6 166,831 + RR42_RS21575 0.79 -2.3 166,831 + RR42_RS21575 0.79 +1.8 166,831 + RR42_RS21575 0.79 -0.8 166,831 + RR42_RS21575 0.79 -0.2 166,832 - RR42_RS21575 0.80 -0.0 166,832 - RR42_RS21575 0.80 +0.3 166,832 - RR42_RS21575 0.80 +0.5 166,832 - RR42_RS21575 0.80 -1.0 166,832 - RR42_RS21575 0.80 +0.3 166,934 - -0.7 166,998 + -1.0 167,073 + -0.2 167,074 - +0.6 167,074 - +0.2 167,081 + +0.9 167,082 - +2.4 167,117 + -1.0 167,264 + RR42_RS21580 0.21 +0.7 167,468 + RR42_RS21580 0.38 -2.3 167,468 + RR42_RS21580 0.38 +1.4 167,469 - RR42_RS21580 0.38 -0.3 167,469 - RR42_RS21580 0.38 -0.2 167,531 + RR42_RS21580 0.43 +0.6 167,943 + RR42_RS21580 0.78 -1.5 167,943 + RR42_RS21580 0.78 +2.4 167,943 + RR42_RS21580 0.78 -1.0 167,944 - RR42_RS21580 0.78 -1.1 167,944 - RR42_RS21580 0.78 +0.0
Or see this region's nucleotide sequence