Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05570 and RR42_RS05575 are separated by 18 nucleotides RR42_RS05575 and RR42_RS05580 are separated by 22 nucleotides RR42_RS05580 and RR42_RS05585 are separated by 52 nucleotides
RR42_RS05570: RR42_RS05570 - NADH:ubiquinone oxidoreductase subunit M, at 1,227,161 to 1,228,627
_RS05570
RR42_RS05575: RR42_RS05575 - NADH:ubiquinone oxidoreductase subunit N, at 1,228,646 to 1,230,121
_RS05575
RR42_RS05580: RR42_RS05580 - membrane protein, at 1,230,144 to 1,230,452
_RS05580
RR42_RS05585: RR42_RS05585 - ADP-ribose pyrophosphatase, at 1,230,505 to 1,231,128
_RS05585
Position (kb) 1228
1229
1230
1231 Strain fitness (log2 ratio) -1
0
1
2 at 1230.143 kb on + strand at 1230.369 kb on + strand, within RR42_RS05580 at 1230.631 kb on - strand, within RR42_RS05585 at 1230.671 kb on - strand, within RR42_RS05585 at 1230.742 kb on + strand, within RR42_RS05585 at 1230.871 kb on + strand, within RR42_RS05585 at 1230.921 kb on + strand, within RR42_RS05585 at 1230.922 kb on - strand, within RR42_RS05585 at 1231.115 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,230,143 + +0.9 1,230,369 + RR42_RS05580 0.73 +1.4 1,230,631 - RR42_RS05585 0.20 +0.1 1,230,671 - RR42_RS05585 0.27 +1.6 1,230,742 + RR42_RS05585 0.38 +1.9 1,230,871 + RR42_RS05585 0.59 -0.9 1,230,921 + RR42_RS05585 0.67 +0.3 1,230,922 - RR42_RS05585 0.67 +0.5 1,231,115 + -1.4
Or see this region's nucleotide sequence