Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04385 and RR42_RS04390 are separated by 104 nucleotides RR42_RS04390 and RR42_RS04395 are separated by 167 nucleotides RR42_RS04395 and RR42_RS04400 are separated by 30 nucleotides
RR42_RS04385: RR42_RS04385 - amino acid dehydrogenase, at 975,678 to 976,940
_RS04385
RR42_RS04390: RR42_RS04390 - ABC transporter, at 977,045 to 977,965
_RS04390
RR42_RS04395: RR42_RS04395 - cysteine ABC transporter substrate-binding protein, at 978,133 to 978,930
_RS04395
RR42_RS04400: RR42_RS04400 - ABC transporter permease, at 978,961 to 979,620
_RS04400
Position (kb)
977
978 Strain fitness (log2 ratio)
-1
0
1
2 at 976.144 kb on - strand, within RR42_RS04385 at 976.394 kb on + strand at 976.394 kb on + strand, within RR42_RS04385 at 976.395 kb on - strand, within RR42_RS04385 at 976.506 kb on + strand, within RR42_RS04385 at 976.506 kb on + strand, within RR42_RS04385 at 976.507 kb on - strand, within RR42_RS04385 at 976.585 kb on - strand, within RR42_RS04385 at 976.585 kb on - strand, within RR42_RS04385 at 976.977 kb on - strand at 977.011 kb on - strand at 977.044 kb on + strand at 977.152 kb on + strand, within RR42_RS04390 at 977.152 kb on + strand, within RR42_RS04390 at 977.222 kb on + strand, within RR42_RS04390 at 977.222 kb on + strand, within RR42_RS04390 at 977.223 kb on - strand, within RR42_RS04390 at 977.224 kb on + strand, within RR42_RS04390 at 977.250 kb on - strand, within RR42_RS04390 at 977.317 kb on + strand, within RR42_RS04390 at 977.684 kb on + strand, within RR42_RS04390 at 977.684 kb on + strand, within RR42_RS04390 at 977.757 kb on - strand, within RR42_RS04390 at 977.757 kb on - strand, within RR42_RS04390 at 977.757 kb on - strand, within RR42_RS04390 at 977.852 kb on + strand, within RR42_RS04390 at 977.853 kb on - strand, within RR42_RS04390 at 977.853 kb on - strand, within RR42_RS04390 at 977.903 kb on + strand at 977.933 kb on + strand at 977.933 kb on + strand at 977.944 kb on + strand at 977.964 kb on - strand at 978.035 kb on + strand at 978.132 kb on + strand at 978.132 kb on + strand at 978.133 kb on - strand at 978.133 kb on - strand at 978.133 kb on - strand at 978.230 kb on + strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.287 kb on - strand, within RR42_RS04395 at 978.490 kb on + strand, within RR42_RS04395 at 978.490 kb on + strand, within RR42_RS04395 at 978.491 kb on - strand, within RR42_RS04395 at 978.497 kb on - strand, within RR42_RS04395 at 978.598 kb on + strand, within RR42_RS04395 at 978.599 kb on - strand, within RR42_RS04395 at 978.599 kb on - strand, within RR42_RS04395 at 978.661 kb on + strand, within RR42_RS04395
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 976,144 - RR42_RS04385 0.37 +0.7 976,394 + +0.3 976,394 + RR42_RS04385 0.57 +0.4 976,395 - RR42_RS04385 0.57 +0.3 976,506 + RR42_RS04385 0.66 +0.3 976,506 + RR42_RS04385 0.66 +0.5 976,507 - RR42_RS04385 0.66 +0.9 976,585 - RR42_RS04385 0.72 +0.3 976,585 - RR42_RS04385 0.72 -0.3 976,977 - +0.1 977,011 - +0.8 977,044 + +0.4 977,152 + RR42_RS04390 0.12 -1.2 977,152 + RR42_RS04390 0.12 -1.0 977,222 + RR42_RS04390 0.19 +0.5 977,222 + RR42_RS04390 0.19 +2.3 977,223 - RR42_RS04390 0.19 +0.2 977,224 + RR42_RS04390 0.19 +1.6 977,250 - RR42_RS04390 0.22 +0.1 977,317 + RR42_RS04390 0.30 +1.3 977,684 + RR42_RS04390 0.69 +1.5 977,684 + RR42_RS04390 0.69 -1.0 977,757 - RR42_RS04390 0.77 -0.8 977,757 - RR42_RS04390 0.77 +0.8 977,757 - RR42_RS04390 0.77 -1.3 977,852 + RR42_RS04390 0.88 -1.4 977,853 - RR42_RS04390 0.88 +0.2 977,853 - RR42_RS04390 0.88 -0.5 977,903 + +0.9 977,933 + +1.0 977,933 + +0.1 977,944 + -0.1 977,964 - +0.8 978,035 + +0.6 978,132 + -0.7 978,132 + +0.9 978,133 - -0.5 978,133 - +0.5 978,133 - -0.2 978,230 + RR42_RS04395 0.12 -1.1 978,272 - RR42_RS04395 0.17 -0.5 978,272 - RR42_RS04395 0.17 +0.4 978,272 - RR42_RS04395 0.17 -1.3 978,272 - RR42_RS04395 0.17 +0.8 978,287 - RR42_RS04395 0.19 -0.0 978,490 + RR42_RS04395 0.45 -1.1 978,490 + RR42_RS04395 0.45 -1.2 978,491 - RR42_RS04395 0.45 -0.3 978,497 - RR42_RS04395 0.46 -0.4 978,598 + RR42_RS04395 0.58 -1.3 978,599 - RR42_RS04395 0.58 -0.8 978,599 - RR42_RS04395 0.58 -0.4 978,661 + RR42_RS04395 0.66 +0.3
Or see this region's nucleotide sequence