Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02485 and RR42_RS02490 overlap by 16 nucleotides RR42_RS02490 and RR42_RS02495 are separated by 141 nucleotides RR42_RS02495 and RR42_RS02500 are separated by 369 nucleotides
RR42_RS02485: RR42_RS02485 - short-chain dehydrogenase, at 558,270 to 558,869
_RS02485
RR42_RS02490: RR42_RS02490 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 558,854 to 559,411
_RS02490
RR42_RS02495: RR42_RS02495 - cardiolipin synthase; exported protein, at 559,553 to 560,872
_RS02495
RR42_RS02500: RR42_RS02500 - TetR family transcriptional regulator, at 561,242 to 561,832
_RS02500
Position (kb) 559
560
561 Strain fitness (log2 ratio) -2
-1
0
1
2 at 558.736 kb on + strand, within RR42_RS02485 at 558.736 kb on + strand, within RR42_RS02485 at 558.736 kb on + strand, within RR42_RS02485 at 558.737 kb on - strand, within RR42_RS02485 at 558.760 kb on - strand, within RR42_RS02485 at 558.855 kb on + strand at 558.976 kb on + strand, within RR42_RS02490 at 559.101 kb on + strand, within RR42_RS02490 at 559.101 kb on + strand, within RR42_RS02490 at 559.102 kb on - strand, within RR42_RS02490 at 559.449 kb on - strand at 559.449 kb on - strand at 559.790 kb on + strand, within RR42_RS02495 at 559.790 kb on + strand, within RR42_RS02495 at 559.791 kb on - strand, within RR42_RS02495 at 559.791 kb on - strand, within RR42_RS02495 at 559.791 kb on - strand, within RR42_RS02495 at 559.797 kb on - strand, within RR42_RS02495 at 559.825 kb on - strand, within RR42_RS02495 at 559.953 kb on + strand, within RR42_RS02495 at 559.953 kb on + strand, within RR42_RS02495 at 560.190 kb on + strand, within RR42_RS02495 at 560.406 kb on - strand, within RR42_RS02495 at 560.406 kb on - strand, within RR42_RS02495 at 560.406 kb on - strand, within RR42_RS02495 at 560.513 kb on + strand, within RR42_RS02495 at 560.513 kb on + strand, within RR42_RS02495 at 560.513 kb on + strand, within RR42_RS02495 at 560.532 kb on - strand, within RR42_RS02495 at 560.690 kb on + strand, within RR42_RS02495 at 560.819 kb on + strand at 560.819 kb on + strand at 560.819 kb on + strand at 560.820 kb on - strand at 560.820 kb on - strand at 560.820 kb on - strand at 560.821 kb on + strand at 560.821 kb on + strand at 560.821 kb on + strand at 560.821 kb on + strand at 560.821 kb on + strand at 560.821 kb on + strand at 560.822 kb on - strand at 560.822 kb on - strand at 560.906 kb on + strand at 560.907 kb on - strand at 560.907 kb on - strand at 561.065 kb on - strand at 561.129 kb on + strand at 561.129 kb on + strand at 561.130 kb on - strand at 561.142 kb on + strand at 561.142 kb on + strand at 561.552 kb on - strand, within RR42_RS02500 at 561.552 kb on - strand, within RR42_RS02500 at 561.735 kb on + strand, within RR42_RS02500 at 561.805 kb on + strand at 561.821 kb on + strand at 561.826 kb on + strand at 561.826 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 558,736 + RR42_RS02485 0.78 -0.3 558,736 + RR42_RS02485 0.78 -0.2 558,736 + RR42_RS02485 0.78 -1.3 558,737 - RR42_RS02485 0.78 -1.0 558,760 - RR42_RS02485 0.82 +0.4 558,855 + +1.4 558,976 + RR42_RS02490 0.22 -0.2 559,101 + RR42_RS02490 0.44 -0.7 559,101 + RR42_RS02490 0.44 -0.6 559,102 - RR42_RS02490 0.44 +0.2 559,449 - +1.3 559,449 - -0.3 559,790 + RR42_RS02495 0.18 -0.7 559,790 + RR42_RS02495 0.18 -1.2 559,791 - RR42_RS02495 0.18 -0.0 559,791 - RR42_RS02495 0.18 +0.2 559,791 - RR42_RS02495 0.18 +0.1 559,797 - RR42_RS02495 0.18 +1.4 559,825 - RR42_RS02495 0.21 +1.7 559,953 + RR42_RS02495 0.30 +1.1 559,953 + RR42_RS02495 0.30 -0.0 560,190 + RR42_RS02495 0.48 -1.5 560,406 - RR42_RS02495 0.65 +0.1 560,406 - RR42_RS02495 0.65 +1.3 560,406 - RR42_RS02495 0.65 -0.1 560,513 + RR42_RS02495 0.73 +1.0 560,513 + RR42_RS02495 0.73 -0.3 560,513 + RR42_RS02495 0.73 -0.9 560,532 - RR42_RS02495 0.74 -0.9 560,690 + RR42_RS02495 0.86 +1.1 560,819 + -1.3 560,819 + -1.3 560,819 + +0.8 560,820 - -0.1 560,820 - +1.4 560,820 - -1.6 560,821 + -0.7 560,821 + +0.4 560,821 + +0.9 560,821 + -1.8 560,821 + -0.3 560,821 + +2.2 560,822 - -1.1 560,822 - +0.9 560,906 + -2.2 560,907 - +0.7 560,907 - -0.4 561,065 - +0.3 561,129 + +0.1 561,129 + -0.0 561,130 - -0.6 561,142 + +1.1 561,142 + +0.3 561,552 - RR42_RS02500 0.52 -0.4 561,552 - RR42_RS02500 0.52 -2.0 561,735 + RR42_RS02500 0.83 -1.7 561,805 + -0.6 561,821 + -0.9 561,826 + -0.9 561,826 + +1.4
Or see this region's nucleotide sequence