Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02165 and RR42_RS02170 are separated by 215 nucleotides RR42_RS02170 and RR42_RS02175 are separated by 20 nucleotides RR42_RS02175 and RR42_RS02180 are separated by 8 nucleotides
RR42_RS02165: RR42_RS02165 - LysR family transcriptional regulator, at 481,318 to 482,256
_RS02165
RR42_RS02170: RR42_RS02170 - hypothetical protein, at 482,472 to 483,209
_RS02170
RR42_RS02175: RR42_RS02175 - acetolactate synthase, at 483,230 to 484,855
_RS02175
RR42_RS02180: RR42_RS02180 - ABC transporter substrate-binding protein, at 484,864 to 485,850
_RS02180
Position (kb)
483
484
485 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 482.261 kb on - strand at 482.293 kb on + strand at 482.360 kb on - strand at 482.397 kb on + strand at 482.397 kb on + strand at 482.397 kb on + strand at 482.397 kb on + strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.398 kb on - strand at 482.399 kb on - strand at 482.435 kb on + strand at 482.521 kb on - strand at 482.521 kb on - strand at 482.522 kb on + strand at 482.869 kb on + strand, within RR42_RS02170 at 482.869 kb on + strand, within RR42_RS02170 at 482.872 kb on + strand, within RR42_RS02170 at 482.916 kb on + strand, within RR42_RS02170 at 482.917 kb on - strand, within RR42_RS02170 at 482.925 kb on + strand, within RR42_RS02170 at 482.925 kb on + strand, within RR42_RS02170 at 482.925 kb on + strand, within RR42_RS02170 at 483.033 kb on + strand, within RR42_RS02170 at 483.034 kb on - strand, within RR42_RS02170 at 483.034 kb on - strand, within RR42_RS02170 at 483.041 kb on + strand, within RR42_RS02170 at 483.093 kb on + strand, within RR42_RS02170 at 483.358 kb on - strand at 483.387 kb on + strand at 483.433 kb on - strand, within RR42_RS02175 at 483.454 kb on - strand, within RR42_RS02175 at 484.073 kb on + strand, within RR42_RS02175 at 484.073 kb on + strand, within RR42_RS02175 at 484.074 kb on - strand, within RR42_RS02175 at 484.074 kb on - strand, within RR42_RS02175 at 484.597 kb on - strand, within RR42_RS02175 at 484.788 kb on - strand at 484.788 kb on - strand at 484.865 kb on + strand at 484.866 kb on - strand at 484.904 kb on + strand at 484.904 kb on + strand at 484.905 kb on - strand at 485.072 kb on + strand, within RR42_RS02180 at 485.101 kb on + strand, within RR42_RS02180 at 485.202 kb on + strand, within RR42_RS02180 at 485.257 kb on + strand, within RR42_RS02180 at 485.268 kb on + strand, within RR42_RS02180 at 485.269 kb on - strand, within RR42_RS02180 at 485.300 kb on - strand, within RR42_RS02180 at 485.300 kb on - strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.384 kb on + strand, within RR42_RS02180 at 485.385 kb on - strand, within RR42_RS02180 at 485.385 kb on - strand, within RR42_RS02180 at 485.519 kb on + strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.520 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.736 kb on - strand, within RR42_RS02180 at 485.751 kb on + strand, within RR42_RS02180
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 482,261 - +1.1 482,293 + -0.8 482,360 - -1.8 482,397 + +0.7 482,397 + +0.6 482,397 + +0.6 482,397 + +0.4 482,398 - -0.0 482,398 - -0.1 482,398 - -0.6 482,398 - +1.3 482,398 - -1.2 482,399 - +0.9 482,435 + -1.8 482,521 - -0.3 482,521 - +1.9 482,522 + -0.4 482,869 + RR42_RS02170 0.54 +0.8 482,869 + RR42_RS02170 0.54 +0.5 482,872 + RR42_RS02170 0.54 +0.4 482,916 + RR42_RS02170 0.60 +0.4 482,917 - RR42_RS02170 0.60 +0.2 482,925 + RR42_RS02170 0.61 -0.3 482,925 + RR42_RS02170 0.61 +0.4 482,925 + RR42_RS02170 0.61 +0.6 483,033 + RR42_RS02170 0.76 -0.6 483,034 - RR42_RS02170 0.76 +0.3 483,034 - RR42_RS02170 0.76 +0.2 483,041 + RR42_RS02170 0.77 -0.8 483,093 + RR42_RS02170 0.84 -1.7 483,358 - +0.4 483,387 + -0.6 483,433 - RR42_RS02175 0.12 +1.0 483,454 - RR42_RS02175 0.14 -0.7 484,073 + RR42_RS02175 0.52 -0.3 484,073 + RR42_RS02175 0.52 -1.0 484,074 - RR42_RS02175 0.52 +1.2 484,074 - RR42_RS02175 0.52 +0.5 484,597 - RR42_RS02175 0.84 +0.2 484,788 - -0.6 484,788 - +0.7 484,865 + +0.5 484,866 - -0.4 484,904 + +0.6 484,904 + +0.2 484,905 - -0.5 485,072 + RR42_RS02180 0.21 +2.5 485,101 + RR42_RS02180 0.24 -0.3 485,202 + RR42_RS02180 0.34 +0.0 485,257 + RR42_RS02180 0.40 +0.9 485,268 + RR42_RS02180 0.41 +2.1 485,269 - RR42_RS02180 0.41 -0.1 485,300 - RR42_RS02180 0.44 -0.6 485,300 - RR42_RS02180 0.44 -0.9 485,384 + RR42_RS02180 0.53 +0.3 485,384 + RR42_RS02180 0.53 -0.1 485,384 + RR42_RS02180 0.53 -0.2 485,384 + RR42_RS02180 0.53 -0.6 485,385 - RR42_RS02180 0.53 +0.0 485,385 - RR42_RS02180 0.53 +1.4 485,519 + RR42_RS02180 0.66 +1.9 485,520 - RR42_RS02180 0.66 -0.6 485,520 - RR42_RS02180 0.66 +0.2 485,520 - RR42_RS02180 0.66 -0.1 485,736 - RR42_RS02180 0.88 -0.1 485,736 - RR42_RS02180 0.88 +1.5 485,736 - RR42_RS02180 0.88 -0.2 485,751 + RR42_RS02180 0.90 -0.9
Or see this region's nucleotide sequence