Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01330 and RR42_RS01335 are separated by 222 nucleotides RR42_RS01335 and RR42_RS01340 are separated by 39 nucleotides
RR42_RS01330: RR42_RS01330 - Fis family transcriptional regulator, at 301,044 to 302,765
_RS01330
RR42_RS01335: RR42_RS01335 - methylmalonyl-CoA carboxyltransferase, at 302,988 to 304,535
_RS01335
RR42_RS01340: RR42_RS01340 - hypothetical protein, at 304,575 to 305,597
_RS01340
Position (kb) 302
303
304
305 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 302.304 kb on + strand, within RR42_RS01330 at 302.740 kb on - strand at 302.740 kb on - strand at 302.767 kb on - strand at 302.873 kb on + strand at 302.873 kb on + strand at 302.874 kb on - strand at 302.938 kb on + strand at 302.939 kb on - strand at 302.951 kb on - strand at 302.963 kb on + strand at 303.172 kb on - strand, within RR42_RS01335 at 303.420 kb on + strand, within RR42_RS01335 at 303.547 kb on - strand, within RR42_RS01335 at 303.619 kb on + strand, within RR42_RS01335 at 303.620 kb on - strand, within RR42_RS01335 at 303.656 kb on + strand, within RR42_RS01335 at 303.733 kb on - strand, within RR42_RS01335 at 303.844 kb on - strand, within RR42_RS01335 at 303.844 kb on - strand, within RR42_RS01335 at 304.161 kb on + strand, within RR42_RS01335 at 304.235 kb on + strand, within RR42_RS01335 at 304.236 kb on - strand, within RR42_RS01335 at 304.236 kb on - strand, within RR42_RS01335 at 304.236 kb on - strand, within RR42_RS01335 at 304.317 kb on + strand, within RR42_RS01335 at 304.317 kb on + strand, within RR42_RS01335 at 304.317 kb on + strand, within RR42_RS01335 at 304.318 kb on - strand, within RR42_RS01335 at 304.568 kb on + strand at 304.568 kb on + strand at 304.569 kb on - strand at 304.569 kb on - strand at 304.569 kb on - strand at 305.120 kb on + strand, within RR42_RS01340 at 305.120 kb on + strand, within RR42_RS01340 at 305.120 kb on + strand, within RR42_RS01340 at 305.120 kb on + strand, within RR42_RS01340 at 305.120 kb on + strand, within RR42_RS01340 at 305.121 kb on - strand, within RR42_RS01340 at 305.168 kb on + strand, within RR42_RS01340 at 305.175 kb on + strand, within RR42_RS01340 at 305.175 kb on + strand, within RR42_RS01340 at 305.176 kb on - strand, within RR42_RS01340 at 305.361 kb on + strand, within RR42_RS01340 at 305.388 kb on + strand, within RR42_RS01340 at 305.388 kb on + strand, within RR42_RS01340 at 305.388 kb on + strand, within RR42_RS01340 at 305.389 kb on - strand, within RR42_RS01340 at 305.389 kb on - strand, within RR42_RS01340
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 302,304 + RR42_RS01330 0.73 -0.0 302,740 - +2.4 302,740 - -1.7 302,767 - -1.3 302,873 + -0.3 302,873 + -0.6 302,874 - -0.1 302,938 + -2.0 302,939 - -1.6 302,951 - +0.7 302,963 + -1.6 303,172 - RR42_RS01335 0.12 -0.4 303,420 + RR42_RS01335 0.28 +0.8 303,547 - RR42_RS01335 0.36 +0.5 303,619 + RR42_RS01335 0.41 -0.3 303,620 - RR42_RS01335 0.41 +0.9 303,656 + RR42_RS01335 0.43 +0.3 303,733 - RR42_RS01335 0.48 -2.6 303,844 - RR42_RS01335 0.55 -1.1 303,844 - RR42_RS01335 0.55 -0.1 304,161 + RR42_RS01335 0.76 +0.9 304,235 + RR42_RS01335 0.81 -0.4 304,236 - RR42_RS01335 0.81 +1.5 304,236 - RR42_RS01335 0.81 +0.5 304,236 - RR42_RS01335 0.81 +0.1 304,317 + RR42_RS01335 0.86 -1.3 304,317 + RR42_RS01335 0.86 -0.7 304,317 + RR42_RS01335 0.86 +0.6 304,318 - RR42_RS01335 0.86 -3.7 304,568 + -1.2 304,568 + -0.1 304,569 - -0.0 304,569 - -0.3 304,569 - +2.5 305,120 + RR42_RS01340 0.53 +0.8 305,120 + RR42_RS01340 0.53 -0.2 305,120 + RR42_RS01340 0.53 -0.6 305,120 + RR42_RS01340 0.53 -0.1 305,120 + RR42_RS01340 0.53 -1.5 305,121 - RR42_RS01340 0.53 +0.2 305,168 + RR42_RS01340 0.58 +0.4 305,175 + RR42_RS01340 0.59 -0.1 305,175 + RR42_RS01340 0.59 -0.3 305,176 - RR42_RS01340 0.59 -0.0 305,361 + RR42_RS01340 0.77 -1.4 305,388 + RR42_RS01340 0.79 -2.7 305,388 + RR42_RS01340 0.79 +0.5 305,388 + RR42_RS01340 0.79 +0.7 305,389 - RR42_RS01340 0.80 +0.7 305,389 - RR42_RS01340 0.80 +0.9
Or see this region's nucleotide sequence