Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00885 and RR42_RS00890 are separated by 84 nucleotides RR42_RS00890 and RR42_RS00895 are separated by 48 nucleotides RR42_RS00895 and RR42_RS00900 are separated by 119 nucleotides RR42_RS00900 and RR42_RS00905 overlap by 4 nucleotides
RR42_RS00885: RR42_RS00885 - acetyl-CoA acetyltransferase, at 213,807 to 215,003
_RS00885
RR42_RS00890: RR42_RS00890 - short-chain dehydrogenase, at 215,088 to 215,768
_RS00890
RR42_RS00895: RR42_RS00895 - acyl-CoA dehydrogenase, at 215,817 to 216,947
_RS00895
RR42_RS00900: RR42_RS00900 - 2-hydroxychromene-2-carboxylate isomerase, at 217,067 to 217,768
_RS00900
RR42_RS00905: RR42_RS00905 - haloacid dehalogenase, at 217,765 to 218,454
_RS00905
Position (kb) 215
216
217 Strain fitness (log2 ratio) -2
-1
0
1
2 at 215.067 kb on + strand at 215.305 kb on + strand, within RR42_RS00890 at 215.306 kb on - strand, within RR42_RS00890 at 215.306 kb on - strand, within RR42_RS00890 at 215.313 kb on + strand, within RR42_RS00890 at 215.313 kb on + strand, within RR42_RS00890 at 215.313 kb on + strand, within RR42_RS00890 at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.742 kb on + strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.743 kb on - strand at 215.961 kb on + strand, within RR42_RS00895 at 215.985 kb on + strand, within RR42_RS00895 at 216.007 kb on - strand, within RR42_RS00895 at 216.253 kb on - strand, within RR42_RS00895 at 216.479 kb on + strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.504 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.774 kb on + strand, within RR42_RS00895 at 216.774 kb on + strand, within RR42_RS00895 at 216.775 kb on - strand, within RR42_RS00895 at 216.775 kb on - strand, within RR42_RS00895 at 216.895 kb on - strand at 216.922 kb on + strand at 216.923 kb on - strand at 216.923 kb on - strand at 217.088 kb on + strand at 217.088 kb on + strand at 217.088 kb on + strand at 217.089 kb on - strand at 217.154 kb on + strand, within RR42_RS00900 at 217.250 kb on + strand, within RR42_RS00900 at 217.292 kb on - strand, within RR42_RS00900 at 217.299 kb on - strand, within RR42_RS00900 at 217.730 kb on - strand at 217.899 kb on + strand, within RR42_RS00905 at 217.900 kb on - strand, within RR42_RS00905
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 215,067 + +0.3 215,305 + RR42_RS00890 0.32 +0.3 215,306 - RR42_RS00890 0.32 +0.4 215,306 - RR42_RS00890 0.32 +0.5 215,313 + RR42_RS00890 0.33 +0.3 215,313 + RR42_RS00890 0.33 +0.4 215,313 + RR42_RS00890 0.33 -2.5 215,742 + -0.1 215,742 + +0.7 215,742 + +0.2 215,742 + -0.4 215,742 + -0.1 215,742 + -0.1 215,743 - +1.8 215,743 - -0.3 215,743 - -1.2 215,743 - +0.1 215,743 - -0.5 215,961 + RR42_RS00895 0.13 -1.3 215,985 + RR42_RS00895 0.15 -0.1 216,007 - RR42_RS00895 0.17 +0.6 216,253 - RR42_RS00895 0.39 -0.1 216,479 + RR42_RS00895 0.59 -0.3 216,480 - RR42_RS00895 0.59 +0.2 216,480 - RR42_RS00895 0.59 +0.8 216,480 - RR42_RS00895 0.59 -1.3 216,504 + RR42_RS00895 0.61 -0.6 216,600 + RR42_RS00895 0.69 +0.7 216,600 + RR42_RS00895 0.69 -0.3 216,600 + RR42_RS00895 0.69 -0.1 216,774 + RR42_RS00895 0.85 -0.2 216,774 + RR42_RS00895 0.85 -2.5 216,775 - RR42_RS00895 0.85 -0.3 216,775 - RR42_RS00895 0.85 +0.9 216,895 - +1.2 216,922 + +0.1 216,923 - +0.9 216,923 - +0.5 217,088 + -2.2 217,088 + +0.6 217,088 + -0.3 217,089 - -1.9 217,154 + RR42_RS00900 0.12 -1.4 217,250 + RR42_RS00900 0.26 -1.3 217,292 - RR42_RS00900 0.32 +1.8 217,299 - RR42_RS00900 0.33 -1.1 217,730 - -1.1 217,899 + RR42_RS00905 0.19 -0.1 217,900 - RR42_RS00905 0.20 +0.5
Or see this region's nucleotide sequence