Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03845 and MMP_RS08990 are separated by 27 nucleotides MMP_RS08990 and MMP_RS03850 are separated by 125 nucleotides MMP_RS03850 and MMP_RS03855 are separated by 24 nucleotides
MMP_RS03845: MMP_RS03845 - DNA adenine methylase, at 727,879 to 728,799
_RS03845
MMP_RS08990: MMP_RS08990 - hypothetical protein, at 728,827 to 728,997
_RS08990
MMP_RS03850: MMP_RS03850 - PRC-barrel domain-containing protein, at 729,123 to 729,377
_RS03850
MMP_RS03855: MMP_RS03855 - aspartate dehydrogenase, at 729,402 to 730,205
_RS03855
Position (kb) 728
729 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 727.848 kb on + strand at 727.873 kb on - strand at 727.878 kb on - strand at 727.879 kb on + strand at 728.024 kb on + strand, within MMP_RS03845 at 728.075 kb on - strand, within MMP_RS03845 at 728.198 kb on + strand, within MMP_RS03845 at 728.219 kb on + strand, within MMP_RS03845 at 728.323 kb on + strand, within MMP_RS03845 at 728.382 kb on + strand, within MMP_RS03845 at 728.472 kb on + strand, within MMP_RS03845 at 728.583 kb on + strand, within MMP_RS03845 at 728.602 kb on + strand, within MMP_RS03845 at 728.626 kb on - strand, within MMP_RS03845 at 728.627 kb on + strand, within MMP_RS03845 at 728.627 kb on + strand, within MMP_RS03845 at 728.640 kb on - strand, within MMP_RS03845 at 728.641 kb on + strand, within MMP_RS03845 at 728.658 kb on - strand, within MMP_RS03845 at 728.704 kb on + strand, within MMP_RS03845 at 728.777 kb on - strand at 728.823 kb on - strand at 728.823 kb on - strand at 728.823 kb on - strand at 728.824 kb on + strand at 728.951 kb on + strand, within MMP_RS08990 at 728.952 kb on - strand, within MMP_RS08990 at 728.952 kb on - strand, within MMP_RS08990 at 728.953 kb on + strand, within MMP_RS08990 at 728.984 kb on - strand at 728.985 kb on + strand at 728.985 kb on + strand at 728.985 kb on + strand at 728.997 kb on - strand at 728.997 kb on - strand at 728.998 kb on + strand at 728.998 kb on + strand at 728.998 kb on + strand at 729.003 kb on + strand at 729.033 kb on - strand at 729.034 kb on + strand at 729.042 kb on - strand at 729.042 kb on - strand at 729.042 kb on - strand at 729.047 kb on - strand at 729.047 kb on - strand at 729.048 kb on + strand at 729.070 kb on - strand at 729.076 kb on + strand at 729.080 kb on - strand at 729.089 kb on - strand at 729.120 kb on + strand at 729.120 kb on + strand at 729.168 kb on + strand at 729.178 kb on - strand at 729.180 kb on - strand at 729.180 kb on - strand at 729.181 kb on + strand at 729.181 kb on + strand at 729.219 kb on + strand at 729.384 kb on + strand at 729.387 kb on - strand at 729.388 kb on + strand at 729.419 kb on + strand at 729.755 kb on + strand at 729.760 kb on - strand at 729.986 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 727,848 + -0.5 727,873 - +0.6 727,878 - -0.1 727,879 + +0.2 728,024 + MMP_RS03845 0.16 -0.6 728,075 - MMP_RS03845 0.21 +0.3 728,198 + MMP_RS03845 0.35 -0.2 728,219 + MMP_RS03845 0.37 +0.7 728,323 + MMP_RS03845 0.48 -0.3 728,382 + MMP_RS03845 0.55 -0.8 728,472 + MMP_RS03845 0.64 +0.4 728,583 + MMP_RS03845 0.76 -0.1 728,602 + MMP_RS03845 0.79 +0.9 728,626 - MMP_RS03845 0.81 -0.2 728,627 + MMP_RS03845 0.81 +0.8 728,627 + MMP_RS03845 0.81 +0.5 728,640 - MMP_RS03845 0.83 -0.2 728,641 + MMP_RS03845 0.83 +1.3 728,658 - MMP_RS03845 0.85 -0.3 728,704 + MMP_RS03845 0.90 -0.0 728,777 - -0.4 728,823 - -0.5 728,823 - -0.1 728,823 - +0.3 728,824 + -0.3 728,951 + MMP_RS08990 0.73 +0.0 728,952 - MMP_RS08990 0.73 -0.3 728,952 - MMP_RS08990 0.73 -1.1 728,953 + MMP_RS08990 0.74 -0.2 728,984 - -0.2 728,985 + -0.2 728,985 + -0.1 728,985 + -0.2 728,997 - +0.2 728,997 - +0.7 728,998 + +0.2 728,998 + -0.2 728,998 + -0.3 729,003 + -0.0 729,033 - -0.4 729,034 + +0.0 729,042 - +0.1 729,042 - -0.1 729,042 - -0.2 729,047 - +1.0 729,047 - +0.2 729,048 + +0.1 729,070 - -1.5 729,076 + -1.1 729,080 - +0.2 729,089 - -1.4 729,120 + -0.6 729,120 + -1.2 729,168 + -1.6 729,178 - -1.5 729,180 - -1.1 729,180 - -3.0 729,181 + -1.3 729,181 + -1.7 729,219 + +0.4 729,384 + -1.1 729,387 - -0.0 729,388 + -0.3 729,419 + -0.4 729,755 + -1.0 729,760 - -1.1 729,986 + -0.9
Or see this region's nucleotide sequence