Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06540 and MMP_RS06545 are separated by 10 nucleotides MMP_RS06545 and MMP_RS06550 are separated by 83 nucleotides MMP_RS06550 and MMP_RS06555 are separated by 51 nucleotides MMP_RS06555 and MMP_RS06560 are separated by 262 nucleotides
MMP_RS06540: MMP_RS06540 - 3-methyl-2-oxobutanoate dehydrogenase subunit VorB, at 1,256,753 to 1,257,808
_RS06540
MMP_RS06545: MMP_RS06545 - ferredoxin family protein, at 1,257,819 to 1,258,058
_RS06545
MMP_RS06550: MMP_RS06550 - AMP-binding protein, at 1,258,142 to 1,259,815
_RS06550
MMP_RS06555: MMP_RS06555 - cupin domain-containing protein, at 1,259,867 to 1,260,421
_RS06555
MMP_RS06560: MMP_RS06560 - hypothetical protein, at 1,260,684 to 1,261,130
_RS06560
Position (kb)
1258
1259
1260 Strain fitness (log2 ratio)
-1
0
1 at 1257.685 kb on + strand, within MMP_RS06540 at 1257.705 kb on + strand at 1257.738 kb on - strand at 1257.756 kb on - strand at 1257.756 kb on - strand at 1257.757 kb on + strand at 1257.810 kb on + strand at 1257.812 kb on + strand at 1257.812 kb on + strand at 1257.853 kb on + strand, within MMP_RS06545 at 1257.877 kb on - strand, within MMP_RS06545 at 1257.922 kb on + strand, within MMP_RS06545 at 1257.929 kb on - strand, within MMP_RS06545 at 1257.963 kb on - strand, within MMP_RS06545 at 1258.027 kb on + strand, within MMP_RS06545 at 1258.109 kb on + strand at 1258.130 kb on - strand at 1258.139 kb on - strand at 1258.143 kb on - strand at 1258.320 kb on + strand, within MMP_RS06550 at 1258.406 kb on + strand, within MMP_RS06550 at 1258.670 kb on - strand, within MMP_RS06550 at 1258.676 kb on - strand, within MMP_RS06550 at 1258.733 kb on + strand, within MMP_RS06550 at 1258.733 kb on + strand, within MMP_RS06550 at 1258.908 kb on - strand, within MMP_RS06550 at 1258.937 kb on + strand, within MMP_RS06550 at 1259.003 kb on - strand, within MMP_RS06550 at 1259.449 kb on - strand, within MMP_RS06550 at 1259.611 kb on - strand, within MMP_RS06550 at 1259.660 kb on + strand at 1259.660 kb on + strand at 1259.846 kb on - strand at 1259.860 kb on - strand at 1259.863 kb on + strand at 1259.864 kb on - strand at 1259.869 kb on + strand at 1259.907 kb on + strand at 1259.998 kb on - strand, within MMP_RS06555 at 1260.141 kb on - strand, within MMP_RS06555 at 1260.422 kb on + strand at 1260.422 kb on + strand at 1260.451 kb on + strand at 1260.456 kb on + strand at 1260.460 kb on - strand at 1260.463 kb on + strand at 1260.501 kb on - strand at 1260.501 kb on - strand at 1260.502 kb on + strand at 1260.522 kb on + strand at 1260.535 kb on + strand at 1260.535 kb on + strand at 1260.535 kb on + strand at 1260.549 kb on - strand at 1260.550 kb on + strand at 1260.551 kb on - strand at 1260.552 kb on + strand at 1260.552 kb on + strand at 1260.552 kb on + strand at 1260.564 kb on + strand at 1260.611 kb on - strand at 1260.612 kb on + strand at 1260.612 kb on + strand at 1260.612 kb on + strand at 1260.645 kb on - strand at 1260.648 kb on + strand at 1260.733 kb on - strand, within MMP_RS06560 at 1260.733 kb on - strand, within MMP_RS06560 at 1260.734 kb on + strand, within MMP_RS06560 at 1260.768 kb on - strand, within MMP_RS06560 at 1260.768 kb on - strand, within MMP_RS06560 at 1260.768 kb on - strand, within MMP_RS06560 at 1260.769 kb on + strand, within MMP_RS06560 at 1260.779 kb on + strand, within MMP_RS06560 at 1260.779 kb on + strand, within MMP_RS06560
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,257,685 + MMP_RS06540 0.88 +0.1 1,257,705 + +0.9 1,257,738 - -0.7 1,257,756 - -0.1 1,257,756 - +0.3 1,257,757 + -0.1 1,257,810 + +0.1 1,257,812 + +0.6 1,257,812 + -1.2 1,257,853 + MMP_RS06545 0.14 +0.3 1,257,877 - MMP_RS06545 0.24 -0.6 1,257,922 + MMP_RS06545 0.43 -0.5 1,257,929 - MMP_RS06545 0.46 -0.7 1,257,963 - MMP_RS06545 0.60 +0.2 1,258,027 + MMP_RS06545 0.87 +0.1 1,258,109 + +0.3 1,258,130 - +0.4 1,258,139 - +0.8 1,258,143 - -0.1 1,258,320 + MMP_RS06550 0.11 -0.4 1,258,406 + MMP_RS06550 0.16 -1.1 1,258,670 - MMP_RS06550 0.32 -0.2 1,258,676 - MMP_RS06550 0.32 +0.2 1,258,733 + MMP_RS06550 0.35 -0.3 1,258,733 + MMP_RS06550 0.35 +0.2 1,258,908 - MMP_RS06550 0.46 -0.4 1,258,937 + MMP_RS06550 0.47 -1.0 1,259,003 - MMP_RS06550 0.51 +0.5 1,259,449 - MMP_RS06550 0.78 +0.2 1,259,611 - MMP_RS06550 0.88 -0.1 1,259,660 + +0.5 1,259,660 + +0.6 1,259,846 - +0.3 1,259,860 - -0.6 1,259,863 + -0.3 1,259,864 - -0.2 1,259,869 + -0.0 1,259,907 + +0.5 1,259,998 - MMP_RS06555 0.24 -0.5 1,260,141 - MMP_RS06555 0.49 -0.6 1,260,422 + -0.9 1,260,422 + +0.3 1,260,451 + -0.8 1,260,456 + +0.6 1,260,460 - -1.0 1,260,463 + -0.7 1,260,501 - +0.1 1,260,501 - -0.1 1,260,502 + +0.3 1,260,522 + -0.8 1,260,535 + -0.3 1,260,535 + -0.1 1,260,535 + +0.3 1,260,549 - -0.3 1,260,550 + +0.2 1,260,551 - +0.1 1,260,552 + +0.4 1,260,552 + -0.8 1,260,552 + +0.1 1,260,564 + +0.2 1,260,611 - -0.8 1,260,612 + +0.6 1,260,612 + +0.6 1,260,612 + -0.1 1,260,645 - -0.1 1,260,648 + +1.1 1,260,733 - MMP_RS06560 0.11 +0.2 1,260,733 - MMP_RS06560 0.11 -0.7 1,260,734 + MMP_RS06560 0.11 +0.7 1,260,768 - MMP_RS06560 0.19 -0.3 1,260,768 - MMP_RS06560 0.19 -0.5 1,260,768 - MMP_RS06560 0.19 -0.4 1,260,769 + MMP_RS06560 0.19 +0.5 1,260,779 + MMP_RS06560 0.21 +1.2 1,260,779 + MMP_RS06560 0.21 -0.5
Or see this region's nucleotide sequence