Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06530 and MMP_RS06535 are separated by 84 nucleotides MMP_RS06535 and MMP_RS06540 are separated by 1 nucleotides
MMP_RS06530: MMP_RS06530 - bifunctional hexulose-6-phosphate synthase/ribonuclease regulator, at 1,253,911 to 1,255,227
_RS06530
MMP_RS06535: MMP_RS06535 - 2-oxoacid:acceptor oxidoreductase family protein, at 1,255,312 to 1,256,751
_RS06535
MMP_RS06540: MMP_RS06540 - 3-methyl-2-oxobutanoate dehydrogenase subunit VorB, at 1,256,753 to 1,257,808
_RS06540
Position (kb)
1255
1256
1257 Strain fitness (log2 ratio)
-2
-1
0
1 at 1254.490 kb on - strand, within MMP_RS06530 at 1254.492 kb on - strand, within MMP_RS06530 at 1254.506 kb on + strand, within MMP_RS06530 at 1254.506 kb on + strand, within MMP_RS06530 at 1254.555 kb on - strand, within MMP_RS06530 at 1254.598 kb on + strand, within MMP_RS06530 at 1254.633 kb on + strand, within MMP_RS06530 at 1254.652 kb on + strand, within MMP_RS06530 at 1255.042 kb on + strand, within MMP_RS06530 at 1255.126 kb on + strand at 1255.135 kb on - strand at 1255.313 kb on - strand at 1255.371 kb on - strand at 1255.423 kb on - strand at 1255.480 kb on + strand, within MMP_RS06535 at 1255.766 kb on - strand, within MMP_RS06535 at 1255.766 kb on - strand, within MMP_RS06535 at 1255.912 kb on - strand, within MMP_RS06535 at 1256.538 kb on + strand, within MMP_RS06535 at 1256.538 kb on + strand, within MMP_RS06535 at 1256.584 kb on + strand, within MMP_RS06535 at 1257.081 kb on + strand, within MMP_RS06540 at 1257.087 kb on - strand, within MMP_RS06540 at 1257.685 kb on + strand, within MMP_RS06540 at 1257.705 kb on + strand at 1257.738 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,254,490 - MMP_RS06530 0.44 -1.2 1,254,492 - MMP_RS06530 0.44 +0.0 1,254,506 + MMP_RS06530 0.45 -1.0 1,254,506 + MMP_RS06530 0.45 +0.2 1,254,555 - MMP_RS06530 0.49 -0.6 1,254,598 + MMP_RS06530 0.52 -0.3 1,254,633 + MMP_RS06530 0.55 -1.1 1,254,652 + MMP_RS06530 0.56 -0.7 1,255,042 + MMP_RS06530 0.86 -0.4 1,255,126 + -0.4 1,255,135 - -2.0 1,255,313 - +0.1 1,255,371 - -0.1 1,255,423 - -0.3 1,255,480 + MMP_RS06535 0.12 +0.3 1,255,766 - MMP_RS06535 0.32 -0.8 1,255,766 - MMP_RS06535 0.32 -0.1 1,255,912 - MMP_RS06535 0.42 -0.6 1,256,538 + MMP_RS06535 0.85 -0.7 1,256,538 + MMP_RS06535 0.85 +0.7 1,256,584 + MMP_RS06535 0.88 -0.8 1,257,081 + MMP_RS06540 0.31 +0.2 1,257,087 - MMP_RS06540 0.32 +0.5 1,257,685 + MMP_RS06540 0.88 +0.1 1,257,705 + +0.9 1,257,738 - -0.7
Or see this region's nucleotide sequence