Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06420 and MMP_RS06425 are separated by 33 nucleotides MMP_RS06425 and MMP_RS06430 are separated by 92 nucleotides MMP_RS06430 and MMP_RS06435 are separated by 90 nucleotides MMP_RS06435 and MMP_RS06440 are separated by 22 nucleotides
MMP_RS06420: MMP_RS06420 - formylmethanofuran dehydrogenase subunit A, at 1,231,217 to 1,232,920
_RS06420
MMP_RS06425: MMP_RS06425 - formylmethanofuran dehydrogenase subunit C, at 1,232,954 to 1,233,772
_RS06425
MMP_RS06430: MMP_RS06430 - 6-carboxytetrahydropterin synthase QueD, at 1,233,865 to 1,234,368
_RS06430
MMP_RS06435: MMP_RS06435 - CBS domain-containing protein, at 1,234,459 to 1,235,019
_RS06435
MMP_RS06440: MMP_RS06440 - CBS domain-containing protein, at 1,235,042 to 1,235,881
_RS06440
Position (kb)
1233
1234
1235 Strain fitness (log2 ratio)
-1
0
1
2 at 1233.352 kb on + strand at 1233.708 kb on + strand at 1233.820 kb on - strand at 1233.826 kb on - strand at 1233.827 kb on + strand at 1233.827 kb on + strand at 1233.827 kb on + strand at 1233.828 kb on - strand at 1233.828 kb on - strand at 1233.829 kb on + strand at 1233.832 kb on - strand at 1233.832 kb on - strand at 1233.832 kb on - strand at 1233.833 kb on + strand at 1233.833 kb on + strand at 1233.834 kb on - strand at 1233.835 kb on + strand at 1233.837 kb on - strand at 1233.837 kb on - strand at 1233.847 kb on + strand at 1233.865 kb on + strand at 1233.865 kb on + strand at 1233.947 kb on - strand, within MMP_RS06430 at 1234.046 kb on - strand, within MMP_RS06430 at 1234.135 kb on - strand, within MMP_RS06430 at 1234.139 kb on - strand, within MMP_RS06430 at 1234.140 kb on + strand, within MMP_RS06430 at 1234.236 kb on + strand, within MMP_RS06430 at 1234.244 kb on + strand, within MMP_RS06430 at 1234.256 kb on + strand, within MMP_RS06430 at 1234.320 kb on + strand at 1234.367 kb on + strand at 1234.405 kb on - strand at 1234.405 kb on - strand at 1234.413 kb on + strand at 1234.740 kb on - strand, within MMP_RS06435 at 1234.741 kb on + strand, within MMP_RS06435 at 1234.763 kb on - strand, within MMP_RS06435 at 1234.796 kb on - strand, within MMP_RS06435 at 1234.809 kb on - strand, within MMP_RS06435 at 1234.814 kb on - strand, within MMP_RS06435 at 1234.825 kb on + strand, within MMP_RS06435 at 1234.879 kb on + strand, within MMP_RS06435 at 1235.086 kb on + strand at 1235.092 kb on - strand at 1235.149 kb on + strand, within MMP_RS06440 at 1235.322 kb on - strand, within MMP_RS06440 at 1235.326 kb on + strand, within MMP_RS06440
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,233,352 + +1.1 1,233,708 + +0.0 1,233,820 - +0.4 1,233,826 - +0.6 1,233,827 + +0.2 1,233,827 + +1.5 1,233,827 + -0.6 1,233,828 - -0.1 1,233,828 - -0.2 1,233,829 + +0.5 1,233,832 - -0.3 1,233,832 - +0.2 1,233,832 - -0.6 1,233,833 + -0.1 1,233,833 + +0.3 1,233,834 - +1.8 1,233,835 + -0.1 1,233,837 - +0.5 1,233,837 - -0.0 1,233,847 + +1.0 1,233,865 + -0.5 1,233,865 + -0.6 1,233,947 - MMP_RS06430 0.16 +0.1 1,234,046 - MMP_RS06430 0.36 -0.3 1,234,135 - MMP_RS06430 0.54 -0.2 1,234,139 - MMP_RS06430 0.54 +0.5 1,234,140 + MMP_RS06430 0.55 -0.8 1,234,236 + MMP_RS06430 0.74 -0.2 1,234,244 + MMP_RS06430 0.75 +0.0 1,234,256 + MMP_RS06430 0.78 -0.6 1,234,320 + -0.3 1,234,367 + -0.4 1,234,405 - -0.4 1,234,405 - +0.4 1,234,413 + +0.1 1,234,740 - MMP_RS06435 0.50 -0.4 1,234,741 + MMP_RS06435 0.50 -0.8 1,234,763 - MMP_RS06435 0.54 -0.6 1,234,796 - MMP_RS06435 0.60 +0.3 1,234,809 - MMP_RS06435 0.62 -0.3 1,234,814 - MMP_RS06435 0.63 -0.8 1,234,825 + MMP_RS06435 0.65 -0.5 1,234,879 + MMP_RS06435 0.75 +0.2 1,235,086 + -0.1 1,235,092 - +0.9 1,235,149 + MMP_RS06440 0.13 -0.6 1,235,322 - MMP_RS06440 0.33 +0.0 1,235,326 + MMP_RS06440 0.34 -0.3
Or see this region's nucleotide sequence