Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05965 and MMP_RS05970 are separated by 13 nucleotides MMP_RS05970 and MMP_RS05975 are separated by 20 nucleotides MMP_RS05975 and MMP_RS05980 are separated by 22 nucleotides MMP_RS05980 and MMP_RS05985 are separated by 18 nucleotides MMP_RS05985 and MMP_RS05990 are separated by 35 nucleotides MMP_RS05990 and MMP_RS05995 are separated by 38 nucleotides
MMP_RS05965: MMP_RS05965 - carboxymuconolactone decarboxylase family protein, at 1,144,326 to 1,144,640
_RS05965
MMP_RS05970: MMP_RS05970 - class II SORL domain-containing protein, at 1,144,654 to 1,145,010
_RS05970
MMP_RS05975: MMP_RS05975 - hypothetical protein, at 1,145,031 to 1,145,297
_RS05975
MMP_RS05980: MMP_RS05980 - ferritin, at 1,145,320 to 1,145,835
_RS05980
MMP_RS05985: MMP_RS05985 - hypothetical protein, at 1,145,854 to 1,146,042
_RS05985
MMP_RS05990: MMP_RS05990 - DsrE family protein, at 1,146,078 to 1,146,428
_RS05990
MMP_RS05995: MMP_RS05995 - FprA family A-type flavoprotein, at 1,146,467 to 1,147,630
_RS05995
Position (kb)
1145
1146 Strain fitness (log2 ratio)
-1
0
1 at 1144.544 kb on + strand, within MMP_RS05965 at 1144.580 kb on + strand, within MMP_RS05965 at 1144.580 kb on + strand, within MMP_RS05965 at 1144.653 kb on + strand at 1144.785 kb on + strand, within MMP_RS05970 at 1144.829 kb on - strand, within MMP_RS05970 at 1144.830 kb on + strand, within MMP_RS05970 at 1144.853 kb on - strand, within MMP_RS05970 at 1145.108 kb on - strand, within MMP_RS05975 at 1145.288 kb on - strand at 1145.292 kb on + strand at 1145.303 kb on + strand at 1145.319 kb on - strand at 1145.319 kb on - strand at 1145.320 kb on + strand at 1145.357 kb on - strand at 1145.357 kb on - strand at 1145.357 kb on - strand at 1145.358 kb on + strand at 1145.358 kb on + strand at 1145.362 kb on + strand at 1145.380 kb on - strand, within MMP_RS05980 at 1145.380 kb on - strand, within MMP_RS05980 at 1145.447 kb on - strand, within MMP_RS05980 at 1145.539 kb on + strand, within MMP_RS05980 at 1145.567 kb on - strand, within MMP_RS05980 at 1145.568 kb on + strand, within MMP_RS05980 at 1145.568 kb on + strand, within MMP_RS05980 at 1145.635 kb on + strand, within MMP_RS05980 at 1145.648 kb on - strand, within MMP_RS05980 at 1145.676 kb on + strand, within MMP_RS05980 at 1145.688 kb on + strand, within MMP_RS05980 at 1145.745 kb on + strand, within MMP_RS05980 at 1145.765 kb on + strand, within MMP_RS05980 at 1145.765 kb on + strand, within MMP_RS05980 at 1145.765 kb on + strand, within MMP_RS05980 at 1145.843 kb on + strand at 1145.859 kb on + strand at 1145.961 kb on + strand, within MMP_RS05985 at 1145.961 kb on + strand, within MMP_RS05985 at 1145.961 kb on + strand, within MMP_RS05985 at 1146.039 kb on + strand at 1146.041 kb on + strand at 1146.052 kb on - strand at 1146.053 kb on + strand at 1146.073 kb on - strand at 1146.105 kb on - strand at 1146.292 kb on - strand, within MMP_RS05990 at 1146.312 kb on + strand, within MMP_RS05990 at 1146.335 kb on - strand, within MMP_RS05990 at 1146.350 kb on + strand, within MMP_RS05990 at 1146.377 kb on - strand, within MMP_RS05990 at 1146.432 kb on - strand at 1146.435 kb on - strand at 1146.435 kb on - strand at 1146.461 kb on - strand at 1146.462 kb on + strand at 1146.573 kb on + strand at 1146.578 kb on + strand at 1146.603 kb on - strand, within MMP_RS05995 at 1146.760 kb on + strand, within MMP_RS05995 at 1146.797 kb on - strand, within MMP_RS05995 at 1146.798 kb on + strand, within MMP_RS05995 at 1146.820 kb on - strand, within MMP_RS05995 at 1146.823 kb on + strand, within MMP_RS05995
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,144,544 + MMP_RS05965 0.69 +0.3 1,144,580 + MMP_RS05965 0.81 +0.7 1,144,580 + MMP_RS05965 0.81 -0.1 1,144,653 + -0.2 1,144,785 + MMP_RS05970 0.37 -0.1 1,144,829 - MMP_RS05970 0.49 +0.8 1,144,830 + MMP_RS05970 0.49 -0.5 1,144,853 - MMP_RS05970 0.56 -0.3 1,145,108 - MMP_RS05975 0.29 +0.0 1,145,288 - -0.2 1,145,292 + -0.0 1,145,303 + +0.2 1,145,319 - -0.5 1,145,319 - +0.2 1,145,320 + -0.3 1,145,357 - +0.6 1,145,357 - +0.8 1,145,357 - -0.5 1,145,358 + -0.3 1,145,358 + +0.5 1,145,362 + +0.2 1,145,380 - MMP_RS05980 0.12 -0.1 1,145,380 - MMP_RS05980 0.12 -0.2 1,145,447 - MMP_RS05980 0.25 -0.4 1,145,539 + MMP_RS05980 0.42 +0.2 1,145,567 - MMP_RS05980 0.48 -0.3 1,145,568 + MMP_RS05980 0.48 -0.8 1,145,568 + MMP_RS05980 0.48 -0.4 1,145,635 + MMP_RS05980 0.61 -0.2 1,145,648 - MMP_RS05980 0.64 -0.1 1,145,676 + MMP_RS05980 0.69 -0.2 1,145,688 + MMP_RS05980 0.71 -0.4 1,145,745 + MMP_RS05980 0.82 +0.6 1,145,765 + MMP_RS05980 0.86 -0.3 1,145,765 + MMP_RS05980 0.86 -0.4 1,145,765 + MMP_RS05980 0.86 +0.2 1,145,843 + -0.1 1,145,859 + -0.2 1,145,961 + MMP_RS05985 0.57 -0.4 1,145,961 + MMP_RS05985 0.57 +0.3 1,145,961 + MMP_RS05985 0.57 +0.5 1,146,039 + -0.3 1,146,041 + -0.3 1,146,052 - -0.1 1,146,053 + +0.8 1,146,073 - -0.5 1,146,105 - +0.3 1,146,292 - MMP_RS05990 0.61 +0.1 1,146,312 + MMP_RS05990 0.67 +0.3 1,146,335 - MMP_RS05990 0.73 -0.4 1,146,350 + MMP_RS05990 0.77 -0.3 1,146,377 - MMP_RS05990 0.85 -0.4 1,146,432 - -0.3 1,146,435 - +0.2 1,146,435 - +0.5 1,146,461 - +0.7 1,146,462 + -0.1 1,146,573 + -0.4 1,146,578 + -0.1 1,146,603 - MMP_RS05995 0.12 -0.3 1,146,760 + MMP_RS05995 0.25 -0.2 1,146,797 - MMP_RS05995 0.28 -0.1 1,146,798 + MMP_RS05995 0.28 +0.3 1,146,820 - MMP_RS05995 0.30 -1.5 1,146,823 + MMP_RS05995 0.31 +0.4
Or see this region's nucleotide sequence