Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05880 and MMP_RS05885 are separated by 175 nucleotides MMP_RS05885 and MMP_RS05890 are separated by 263 nucleotides MMP_RS05890 and MMP_RS05895 are separated by 2 nucleotides MMP_RS05895 and MMP_RS05900 are separated by 35 nucleotides
MMP_RS05880: MMP_RS05880 - thiamine phosphate synthase, at 1,128,854 to 1,129,477
_RS05880
MMP_RS05885: MMP_RS05885 - ferredoxin family protein, at 1,129,653 to 1,129,898
_RS05885
MMP_RS05890: MMP_RS05890 - ATP-dependent helicase, at 1,130,162 to 1,132,729
_RS05890
MMP_RS05895: MMP_RS05895 - metallophosphoesterase, at 1,132,732 to 1,133,442
_RS05895
MMP_RS05900: MMP_RS05900 - tetratricopeptide repeat protein, at 1,133,478 to 1,133,846
_RS05900
Position (kb)
1130
1131
1132
1133 Strain fitness (log2 ratio)
-1
0
1 at 1129.275 kb on - strand at 1129.470 kb on - strand at 1129.514 kb on + strand at 1129.600 kb on + strand at 1129.606 kb on - strand at 1129.607 kb on + strand at 1129.660 kb on - strand at 1129.934 kb on - strand at 1129.937 kb on + strand at 1129.937 kb on + strand at 1129.942 kb on - strand at 1129.986 kb on + strand at 1130.030 kb on + strand at 1130.066 kb on + strand at 1130.191 kb on - strand at 1130.195 kb on + strand at 1130.311 kb on - strand at 1130.364 kb on + strand at 1130.373 kb on + strand at 1130.374 kb on - strand at 1130.374 kb on - strand at 1130.375 kb on + strand at 1130.507 kb on + strand, within MMP_RS05890 at 1130.668 kb on - strand, within MMP_RS05890 at 1130.689 kb on - strand, within MMP_RS05890 at 1130.837 kb on + strand, within MMP_RS05890 at 1130.892 kb on - strand, within MMP_RS05890 at 1130.946 kb on - strand, within MMP_RS05890 at 1130.973 kb on - strand, within MMP_RS05890 at 1130.977 kb on - strand, within MMP_RS05890 at 1131.007 kb on + strand, within MMP_RS05890 at 1131.011 kb on - strand, within MMP_RS05890 at 1131.363 kb on - strand, within MMP_RS05890 at 1131.363 kb on - strand, within MMP_RS05890 at 1131.363 kb on - strand, within MMP_RS05890 at 1131.364 kb on + strand, within MMP_RS05890 at 1131.364 kb on + strand, within MMP_RS05890 at 1131.487 kb on - strand, within MMP_RS05890 at 1131.519 kb on + strand, within MMP_RS05890 at 1131.560 kb on - strand, within MMP_RS05890 at 1131.561 kb on + strand, within MMP_RS05890 at 1131.571 kb on + strand, within MMP_RS05890 at 1131.630 kb on + strand, within MMP_RS05890 at 1131.630 kb on + strand, within MMP_RS05890 at 1131.633 kb on + strand, within MMP_RS05890 at 1131.633 kb on + strand, within MMP_RS05890 at 1131.633 kb on + strand, within MMP_RS05890 at 1131.634 kb on - strand, within MMP_RS05890 at 1131.634 kb on - strand, within MMP_RS05890 at 1131.727 kb on + strand, within MMP_RS05890 at 1131.755 kb on + strand, within MMP_RS05890 at 1131.807 kb on + strand, within MMP_RS05890 at 1132.161 kb on - strand, within MMP_RS05890 at 1132.165 kb on - strand, within MMP_RS05890 at 1132.195 kb on - strand, within MMP_RS05890 at 1132.218 kb on - strand, within MMP_RS05890 at 1132.383 kb on - strand, within MMP_RS05890 at 1132.417 kb on + strand, within MMP_RS05890 at 1132.428 kb on - strand, within MMP_RS05890 at 1132.434 kb on - strand, within MMP_RS05890 at 1132.434 kb on - strand, within MMP_RS05890 at 1132.435 kb on + strand, within MMP_RS05890 at 1132.439 kb on - strand, within MMP_RS05890 at 1132.572 kb on - strand at 1132.573 kb on + strand at 1132.573 kb on + strand at 1132.687 kb on - strand at 1132.688 kb on + strand at 1132.727 kb on - strand at 1132.727 kb on - strand at 1132.739 kb on - strand at 1132.740 kb on + strand at 1132.802 kb on - strand at 1132.865 kb on - strand, within MMP_RS05895 at 1132.866 kb on + strand, within MMP_RS05895 at 1133.037 kb on + strand, within MMP_RS05895 at 1133.057 kb on - strand, within MMP_RS05895 at 1133.058 kb on + strand, within MMP_RS05895 at 1133.058 kb on + strand, within MMP_RS05895 at 1133.070 kb on - strand, within MMP_RS05895 at 1133.071 kb on + strand, within MMP_RS05895 at 1133.072 kb on - strand, within MMP_RS05895 at 1133.073 kb on + strand, within MMP_RS05895 at 1133.076 kb on - strand, within MMP_RS05895 at 1133.085 kb on - strand, within MMP_RS05895 at 1133.095 kb on + strand, within MMP_RS05895 at 1133.107 kb on - strand, within MMP_RS05895 at 1133.180 kb on - strand, within MMP_RS05895 at 1133.214 kb on + strand, within MMP_RS05895 at 1133.214 kb on + strand, within MMP_RS05895 at 1133.292 kb on - strand, within MMP_RS05895 at 1133.371 kb on + strand, within MMP_RS05895 at 1133.382 kb on - strand at 1133.586 kb on - strand, within MMP_RS05900 at 1133.586 kb on - strand, within MMP_RS05900 at 1133.592 kb on - strand, within MMP_RS05900 at 1133.593 kb on + strand, within MMP_RS05900 at 1133.599 kb on - strand, within MMP_RS05900 at 1133.599 kb on - strand, within MMP_RS05900 at 1133.600 kb on + strand, within MMP_RS05900 at 1133.600 kb on + strand, within MMP_RS05900 at 1133.605 kb on - strand, within MMP_RS05900 at 1133.709 kb on - strand, within MMP_RS05900
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,129,275 - -0.1 1,129,470 - -0.5 1,129,514 + -0.3 1,129,600 + -0.1 1,129,606 - -0.5 1,129,607 + -0.6 1,129,660 - -0.1 1,129,934 - +0.6 1,129,937 + -0.4 1,129,937 + +0.3 1,129,942 - -0.5 1,129,986 + -0.4 1,130,030 + -0.5 1,130,066 + -0.2 1,130,191 - -0.1 1,130,195 + -0.4 1,130,311 - +0.3 1,130,364 + +0.1 1,130,373 + -0.0 1,130,374 - +0.3 1,130,374 - -0.0 1,130,375 + +0.0 1,130,507 + MMP_RS05890 0.13 -0.8 1,130,668 - MMP_RS05890 0.20 -0.2 1,130,689 - MMP_RS05890 0.21 +0.3 1,130,837 + MMP_RS05890 0.26 +0.2 1,130,892 - MMP_RS05890 0.28 -0.6 1,130,946 - MMP_RS05890 0.31 -0.7 1,130,973 - MMP_RS05890 0.32 +0.0 1,130,977 - MMP_RS05890 0.32 -0.1 1,131,007 + MMP_RS05890 0.33 -0.3 1,131,011 - MMP_RS05890 0.33 -0.5 1,131,363 - MMP_RS05890 0.47 +0.2 1,131,363 - MMP_RS05890 0.47 -1.0 1,131,363 - MMP_RS05890 0.47 -0.2 1,131,364 + MMP_RS05890 0.47 -0.7 1,131,364 + MMP_RS05890 0.47 -0.1 1,131,487 - MMP_RS05890 0.52 +0.7 1,131,519 + MMP_RS05890 0.53 +0.1 1,131,560 - MMP_RS05890 0.54 +0.0 1,131,561 + MMP_RS05890 0.54 -0.1 1,131,571 + MMP_RS05890 0.55 -0.3 1,131,630 + MMP_RS05890 0.57 +0.2 1,131,630 + MMP_RS05890 0.57 +0.1 1,131,633 + MMP_RS05890 0.57 +0.4 1,131,633 + MMP_RS05890 0.57 -0.2 1,131,633 + MMP_RS05890 0.57 +0.1 1,131,634 - MMP_RS05890 0.57 -0.1 1,131,634 - MMP_RS05890 0.57 +0.1 1,131,727 + MMP_RS05890 0.61 +0.2 1,131,755 + MMP_RS05890 0.62 -0.8 1,131,807 + MMP_RS05890 0.64 -0.5 1,132,161 - MMP_RS05890 0.78 -0.4 1,132,165 - MMP_RS05890 0.78 -0.4 1,132,195 - MMP_RS05890 0.79 -0.5 1,132,218 - MMP_RS05890 0.80 +0.1 1,132,383 - MMP_RS05890 0.86 -0.5 1,132,417 + MMP_RS05890 0.88 -0.7 1,132,428 - MMP_RS05890 0.88 -0.1 1,132,434 - MMP_RS05890 0.88 +0.1 1,132,434 - MMP_RS05890 0.88 +0.2 1,132,435 + MMP_RS05890 0.89 -0.1 1,132,439 - MMP_RS05890 0.89 +0.2 1,132,572 - -0.5 1,132,573 + -0.8 1,132,573 + +0.1 1,132,687 - -0.1 1,132,688 + -0.6 1,132,727 - +0.7 1,132,727 - +0.5 1,132,739 - +0.4 1,132,740 + -0.3 1,132,802 - -0.0 1,132,865 - MMP_RS05895 0.19 +0.2 1,132,866 + MMP_RS05895 0.19 -0.1 1,133,037 + MMP_RS05895 0.43 -0.1 1,133,057 - MMP_RS05895 0.46 +0.2 1,133,058 + MMP_RS05895 0.46 +0.5 1,133,058 + MMP_RS05895 0.46 -0.5 1,133,070 - MMP_RS05895 0.48 -0.7 1,133,071 + MMP_RS05895 0.48 -0.5 1,133,072 - MMP_RS05895 0.48 -0.4 1,133,073 + MMP_RS05895 0.48 -0.1 1,133,076 - MMP_RS05895 0.48 +0.5 1,133,085 - MMP_RS05895 0.50 +1.1 1,133,095 + MMP_RS05895 0.51 +0.3 1,133,107 - MMP_RS05895 0.53 -0.0 1,133,180 - MMP_RS05895 0.63 -0.6 1,133,214 + MMP_RS05895 0.68 -0.3 1,133,214 + MMP_RS05895 0.68 +0.2 1,133,292 - MMP_RS05895 0.79 -0.3 1,133,371 + MMP_RS05895 0.90 +0.9 1,133,382 - +0.2 1,133,586 - MMP_RS05900 0.29 +0.0 1,133,586 - MMP_RS05900 0.29 -0.8 1,133,592 - MMP_RS05900 0.31 -0.6 1,133,593 + MMP_RS05900 0.31 +0.2 1,133,599 - MMP_RS05900 0.33 +0.8 1,133,599 - MMP_RS05900 0.33 +0.8 1,133,600 + MMP_RS05900 0.33 -0.8 1,133,600 + MMP_RS05900 0.33 +1.2 1,133,605 - MMP_RS05900 0.34 -0.2 1,133,709 - MMP_RS05900 0.63 -0.4
Or see this region's nucleotide sequence