Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02470 and MMP_RS02475 are separated by 154 nucleotides MMP_RS02475 and MMP_RS02480 are separated by 98 nucleotides MMP_RS02480 and MMP_RS02485 are separated by 2 nucleotides MMP_RS02485 and MMP_RS02490 overlap by 14 nucleotides MMP_RS02490 and MMP_RS02495 are separated by 11 nucleotides MMP_RS02495 and MMP_RS02500 are separated by 1 nucleotides
MMP_RS02470: MMP_RS02470 - helix-turn-helix transcriptional regulator, at 463,612 to 463,974
_RS02470
MMP_RS02475: MMP_RS02475 - hypothetical protein, at 464,129 to 464,380
_RS02475
MMP_RS02480: MMP_RS02480 - hypothetical protein, at 464,479 to 464,997
_RS02480
MMP_RS02485: MMP_RS02485 - DUF2080 family transposase-associated protein, at 465,000 to 465,224
_RS02485
MMP_RS02490: MMP_RS02490 - hypothetical protein, at 465,211 to 465,405
_RS02490
MMP_RS02495: MMP_RS02495 - hypothetical protein, at 465,417 to 465,800
_RS02495
MMP_RS02500: MMP_RS02500 - minichromosome maintenance protein MCM, at 465,802 to 467,826
_RS02500
Position (kb) 464
465 Strain fitness (log2 ratio) -1
0
1 at 463.481 kb on - strand at 463.499 kb on - strand at 463.509 kb on - strand at 463.510 kb on + strand at 463.517 kb on - strand at 463.517 kb on - strand at 463.527 kb on - strand at 463.531 kb on - strand at 463.532 kb on + strand at 463.532 kb on + strand at 463.547 kb on - strand at 463.551 kb on - strand at 463.551 kb on - strand at 463.551 kb on - strand at 463.637 kb on + strand at 463.676 kb on + strand, within MMP_RS02470 at 463.819 kb on - strand, within MMP_RS02470 at 464.000 kb on + strand at 464.009 kb on - strand at 464.009 kb on - strand at 464.009 kb on - strand at 464.010 kb on + strand at 464.011 kb on - strand at 464.012 kb on + strand at 464.032 kb on - strand at 464.103 kb on + strand at 464.103 kb on + strand at 464.105 kb on - strand at 464.105 kb on - strand at 464.105 kb on - strand at 464.106 kb on + strand at 464.106 kb on + strand at 464.198 kb on - strand, within MMP_RS02475 at 464.199 kb on + strand, within MMP_RS02475 at 464.236 kb on - strand, within MMP_RS02475 at 464.364 kb on + strand at 464.455 kb on - strand at 464.676 kb on + strand, within MMP_RS02480 at 464.825 kb on - strand, within MMP_RS02480 at 465.306 kb on - strand, within MMP_RS02490 at 465.306 kb on - strand, within MMP_RS02490 at 465.307 kb on + strand, within MMP_RS02490 at 465.432 kb on - strand at 465.679 kb on + strand, within MMP_RS02495 at 465.682 kb on - strand, within MMP_RS02495 at 465.851 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 463,481 - +0.7 463,499 - +0.3 463,509 - +0.7 463,510 + -0.1 463,517 - -0.6 463,517 - +0.2 463,527 - +0.2 463,531 - -0.0 463,532 + +0.4 463,532 + +0.6 463,547 - +0.0 463,551 - -0.2 463,551 - +0.3 463,551 - -0.1 463,637 + -0.6 463,676 + MMP_RS02470 0.18 +0.0 463,819 - MMP_RS02470 0.57 +1.4 464,000 + -1.1 464,009 - -0.4 464,009 - -0.4 464,009 - -0.4 464,010 + -0.3 464,011 - -0.7 464,012 + -0.4 464,032 - +1.2 464,103 + +0.1 464,103 + +0.2 464,105 - +0.1 464,105 - -0.4 464,105 - +1.2 464,106 + +0.4 464,106 + -0.3 464,198 - MMP_RS02475 0.27 +0.1 464,199 + MMP_RS02475 0.28 +0.1 464,236 - MMP_RS02475 0.42 +0.5 464,364 + +0.4 464,455 - -0.1 464,676 + MMP_RS02480 0.38 -0.0 464,825 - MMP_RS02480 0.67 -0.3 465,306 - MMP_RS02490 0.49 -0.5 465,306 - MMP_RS02490 0.49 +0.9 465,307 + MMP_RS02490 0.49 -0.3 465,432 - -0.1 465,679 + MMP_RS02495 0.68 -0.8 465,682 - MMP_RS02495 0.69 -0.3 465,851 - -0.1
Or see this region's nucleotide sequence