Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00765 and MMP_RS00770 are separated by 49 nucleotides MMP_RS00770 and MMP_RS00775 are separated by 270 nucleotides
MMP_RS00765: MMP_RS00765 - IMP dehydrogenase, at 139,445 to 140,947
_RS00765
MMP_RS00770: MMP_RS00770 - hypothetical protein, at 140,997 to 141,341
_RS00770
MMP_RS00775: MMP_RS00775 - threonine synthase, at 141,612 to 142,826
_RS00775
Position (kb) 140
141
142 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 140.291 kb on + strand at 140.457 kb on + strand at 140.898 kb on - strand at 140.927 kb on - strand at 140.977 kb on - strand at 140.977 kb on - strand at 140.981 kb on - strand at 140.982 kb on + strand at 141.194 kb on - strand, within MMP_RS00770 at 141.195 kb on + strand, within MMP_RS00770 at 141.226 kb on - strand, within MMP_RS00770 at 141.319 kb on - strand at 141.340 kb on + strand at 141.396 kb on - strand at 141.400 kb on - strand at 141.426 kb on - strand at 141.427 kb on + strand at 141.428 kb on - strand at 141.429 kb on + strand at 141.429 kb on + strand at 141.429 kb on + strand at 141.429 kb on + strand at 141.430 kb on - strand at 141.431 kb on + strand at 141.431 kb on + strand at 141.431 kb on + strand at 141.431 kb on + strand at 141.459 kb on - strand at 141.460 kb on + strand at 141.485 kb on + strand at 141.531 kb on + strand at 141.557 kb on + strand at 141.562 kb on - strand at 141.611 kb on - strand at 141.612 kb on + strand at 141.631 kb on - strand at 141.787 kb on - strand at 142.197 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 140,291 + +0.8 140,457 + +0.5 140,898 - -0.9 140,927 - -0.5 140,977 - +0.1 140,977 - -1.3 140,981 - +0.5 140,982 + -0.5 141,194 - MMP_RS00770 0.57 -0.4 141,195 + MMP_RS00770 0.57 -0.4 141,226 - MMP_RS00770 0.66 +0.2 141,319 - +0.1 141,340 + -0.1 141,396 - +0.1 141,400 - -0.3 141,426 - +0.0 141,427 + +0.5 141,428 - -0.4 141,429 + -0.1 141,429 + -0.0 141,429 + -0.8 141,429 + -0.5 141,430 - +0.1 141,431 + +0.1 141,431 + -0.5 141,431 + -0.2 141,431 + +0.1 141,459 - -0.4 141,460 + +0.4 141,485 + +0.0 141,531 + -0.2 141,557 + +0.6 141,562 - -0.1 141,611 - -0.2 141,612 + +0.3 141,631 - -1.1 141,787 - -3.3 142,197 - -0.2
Or see this region's nucleotide sequence