Experiment: PYE with Gentamicin 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03893 and CCNA_01721 are separated by 22 nucleotides CCNA_01721 and CCNA_01722 overlap by 32 nucleotides CCNA_01722 and CCNA_01723 are separated by 92 nucleotides
CCNA_03893: CCNA_03893 - hypothetical protein, at 1,848,134 to 1,848,526
_03893
CCNA_01721: CCNA_01721 - RNA polymerase ECF sigma factor, at 1,848,549 to 1,849,133
_01721
CCNA_01722: CCNA_01722 - hypothetical protein, at 1,849,102 to 1,849,506
_01722
CCNA_01723: CCNA_01723 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,849,599 to 1,850,426
_01723
Position (kb)
1849
1850 Strain fitness (log2 ratio)
-1
0
1
2 at 1848.189 kb on - strand, within CCNA_03893 at 1848.268 kb on + strand, within CCNA_03893 at 1848.292 kb on + strand, within CCNA_03893 at 1848.323 kb on + strand, within CCNA_03893 at 1848.331 kb on - strand, within CCNA_03893 at 1848.386 kb on + strand, within CCNA_03893 at 1848.461 kb on + strand, within CCNA_03893 at 1848.495 kb on + strand at 1848.495 kb on + strand at 1848.520 kb on + strand at 1848.520 kb on + strand at 1848.536 kb on + strand at 1848.537 kb on + strand at 1848.589 kb on + strand at 1848.597 kb on - strand at 1848.614 kb on - strand, within CCNA_01721 at 1848.629 kb on + strand, within CCNA_01721 at 1848.666 kb on + strand, within CCNA_01721 at 1848.674 kb on - strand, within CCNA_01721 at 1848.675 kb on - strand, within CCNA_01721 at 1848.731 kb on + strand, within CCNA_01721 at 1848.812 kb on + strand, within CCNA_01721 at 1848.812 kb on + strand, within CCNA_01721 at 1848.820 kb on - strand, within CCNA_01721 at 1848.820 kb on - strand, within CCNA_01721 at 1848.827 kb on + strand, within CCNA_01721 at 1848.881 kb on - strand, within CCNA_01721 at 1848.936 kb on + strand, within CCNA_01721 at 1848.936 kb on + strand, within CCNA_01721 at 1848.988 kb on + strand, within CCNA_01721 at 1849.044 kb on + strand, within CCNA_01721 at 1849.052 kb on - strand, within CCNA_01721 at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.130 kb on - strand at 1849.198 kb on + strand at 1849.200 kb on + strand, within CCNA_01722 at 1849.277 kb on + strand, within CCNA_01722 at 1849.282 kb on + strand, within CCNA_01722 at 1849.282 kb on + strand, within CCNA_01722 at 1849.520 kb on + strand at 1850.421 kb on + strand at 1850.454 kb on + strand at 1850.454 kb on + strand at 1850.456 kb on + strand at 1850.462 kb on - strand at 1850.503 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Gentamicin 0.0005 mg/ml remove 1,848,189 - CCNA_03893 0.14 +1.5 1,848,268 + CCNA_03893 0.34 +1.2 1,848,292 + CCNA_03893 0.40 +1.0 1,848,323 + CCNA_03893 0.48 +0.9 1,848,331 - CCNA_03893 0.50 -0.5 1,848,386 + CCNA_03893 0.64 -0.3 1,848,461 + CCNA_03893 0.83 +0.9 1,848,495 + +0.7 1,848,495 + -0.2 1,848,520 + +1.7 1,848,520 + +2.1 1,848,536 + +2.7 1,848,537 + +1.9 1,848,589 + +0.0 1,848,597 - +2.5 1,848,614 - CCNA_01721 0.11 +2.0 1,848,629 + CCNA_01721 0.14 -1.7 1,848,666 + CCNA_01721 0.20 +1.9 1,848,674 - CCNA_01721 0.21 -0.8 1,848,675 - CCNA_01721 0.22 +0.9 1,848,731 + CCNA_01721 0.31 +0.6 1,848,812 + CCNA_01721 0.45 +0.1 1,848,812 + CCNA_01721 0.45 +1.5 1,848,820 - CCNA_01721 0.46 -0.6 1,848,820 - CCNA_01721 0.46 -0.7 1,848,827 + CCNA_01721 0.48 +0.1 1,848,881 - CCNA_01721 0.57 -0.4 1,848,936 + CCNA_01721 0.66 -0.1 1,848,936 + CCNA_01721 0.66 -0.1 1,848,988 + CCNA_01721 0.75 +0.7 1,849,044 + CCNA_01721 0.85 +0.3 1,849,052 - CCNA_01721 0.86 -1.4 1,849,122 + +1.7 1,849,122 + -1.7 1,849,122 + -1.6 1,849,122 + +0.7 1,849,130 - +0.2 1,849,198 + +1.2 1,849,200 + CCNA_01722 0.24 -0.4 1,849,277 + CCNA_01722 0.43 +2.7 1,849,282 + CCNA_01722 0.44 +1.5 1,849,282 + CCNA_01722 0.44 +1.6 1,849,520 + +1.0 1,850,421 + -0.6 1,850,454 + -0.5 1,850,454 + +0.5 1,850,456 + +1.6 1,850,462 - +1.7 1,850,503 + -1.3
Or see this region's nucleotide sequence