Experiment: Recovery broth with Gramicidin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12450 and BBR_RS12455 are separated by 27 nucleotides BBR_RS12455 and BBR_RS12460 are separated by 161 nucleotides
BBR_RS12450: BBR_RS12450 - signal peptidase I, at 542,788 to 543,711
_RS12450
BBR_RS12455: BBR_RS12455 - ribonuclease HII, at 543,739 to 544,596
_RS12455
BBR_RS12460: BBR_RS12460 - LacI family DNA-binding transcriptional regulator, at 544,758 to 545,873
_RS12460
Position (kb) 543
544
545 Strain fitness (log2 ratio) -2
-1
0
1
2 at 542.753 kb on + strand at 542.760 kb on - strand at 542.760 kb on - strand at 542.771 kb on - strand at 542.779 kb on + strand at 542.789 kb on + strand at 542.796 kb on - strand at 542.796 kb on - strand at 542.798 kb on + strand at 542.821 kb on + strand at 542.821 kb on + strand at 542.848 kb on + strand at 542.850 kb on + strand at 542.850 kb on + strand at 542.856 kb on - strand at 542.875 kb on + strand at 542.875 kb on + strand at 543.000 kb on + strand, within BBR_RS12450 at 543.017 kb on + strand, within BBR_RS12450 at 543.036 kb on + strand, within BBR_RS12450 at 543.102 kb on - strand, within BBR_RS12450 at 543.104 kb on + strand, within BBR_RS12450 at 543.603 kb on + strand, within BBR_RS12450 at 543.667 kb on - strand at 543.669 kb on + strand at 543.669 kb on + strand at 543.669 kb on + strand at 543.669 kb on + strand at 543.680 kb on + strand at 543.752 kb on + strand at 543.754 kb on - strand at 543.793 kb on - strand at 543.834 kb on - strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.982 kb on - strand, within BBR_RS12455 at 543.996 kb on + strand, within BBR_RS12455 at 543.996 kb on + strand, within BBR_RS12455 at 543.996 kb on + strand, within BBR_RS12455 at 544.065 kb on + strand, within BBR_RS12455 at 544.065 kb on + strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.082 kb on - strand, within BBR_RS12455 at 544.082 kb on - strand, within BBR_RS12455 at 544.082 kb on - strand, within BBR_RS12455 at 544.084 kb on + strand, within BBR_RS12455 at 544.084 kb on + strand, within BBR_RS12455 at 544.092 kb on - strand, within BBR_RS12455 at 544.092 kb on - strand, within BBR_RS12455 at 544.101 kb on + strand, within BBR_RS12455 at 544.101 kb on + strand, within BBR_RS12455 at 544.109 kb on - strand, within BBR_RS12455 at 544.136 kb on + strand, within BBR_RS12455 at 544.138 kb on - strand, within BBR_RS12455 at 544.150 kb on - strand, within BBR_RS12455 at 544.154 kb on - strand, within BBR_RS12455 at 544.164 kb on - strand, within BBR_RS12455 at 544.170 kb on + strand, within BBR_RS12455 at 544.176 kb on + strand, within BBR_RS12455 at 544.178 kb on - strand, within BBR_RS12455 at 544.181 kb on + strand, within BBR_RS12455 at 544.181 kb on + strand, within BBR_RS12455 at 544.187 kb on + strand, within BBR_RS12455 at 544.224 kb on - strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.254 kb on - strand, within BBR_RS12455 at 544.256 kb on - strand, within BBR_RS12455 at 544.256 kb on - strand, within BBR_RS12455 at 544.266 kb on + strand, within BBR_RS12455 at 544.272 kb on + strand, within BBR_RS12455 at 544.301 kb on + strand, within BBR_RS12455 at 544.324 kb on + strand, within BBR_RS12455 at 544.326 kb on - strand, within BBR_RS12455 at 544.334 kb on + strand, within BBR_RS12455 at 544.337 kb on - strand, within BBR_RS12455 at 544.380 kb on + strand, within BBR_RS12455 at 544.380 kb on + strand, within BBR_RS12455 at 544.380 kb on + strand, within BBR_RS12455 at 544.381 kb on + strand, within BBR_RS12455 at 544.388 kb on - strand, within BBR_RS12455 at 544.395 kb on + strand, within BBR_RS12455 at 544.398 kb on - strand, within BBR_RS12455 at 544.403 kb on - strand, within BBR_RS12455 at 544.415 kb on + strand, within BBR_RS12455 at 544.425 kb on - strand, within BBR_RS12455 at 544.448 kb on + strand, within BBR_RS12455 at 544.460 kb on + strand, within BBR_RS12455 at 544.460 kb on + strand, within BBR_RS12455 at 544.466 kb on - strand, within BBR_RS12455 at 544.499 kb on - strand, within BBR_RS12455 at 544.503 kb on - strand, within BBR_RS12455 at 544.506 kb on + strand, within BBR_RS12455 at 544.512 kb on + strand at 544.521 kb on - strand at 544.521 kb on - strand at 544.554 kb on - strand at 544.564 kb on + strand at 544.570 kb on - strand at 544.570 kb on - strand at 544.598 kb on - strand at 544.608 kb on + strand at 544.608 kb on + strand at 544.611 kb on - strand at 544.624 kb on + strand at 544.713 kb on - strand at 544.725 kb on + strand at 544.739 kb on + strand at 544.746 kb on + strand at 544.805 kb on + strand at 544.833 kb on + strand at 544.833 kb on + strand at 544.841 kb on - strand at 544.866 kb on + strand at 544.866 kb on - strand at 544.868 kb on + strand at 544.868 kb on + strand at 544.895 kb on - strand, within BBR_RS12460 at 544.900 kb on + strand, within BBR_RS12460 at 544.918 kb on - strand, within BBR_RS12460 at 544.918 kb on - strand, within BBR_RS12460 at 544.933 kb on - strand, within BBR_RS12460 at 544.955 kb on - strand, within BBR_RS12460 at 544.995 kb on - strand, within BBR_RS12460 at 545.044 kb on + strand, within BBR_RS12460 at 545.044 kb on + strand, within BBR_RS12460 at 545.052 kb on - strand, within BBR_RS12460 at 545.054 kb on + strand, within BBR_RS12460 at 545.083 kb on - strand, within BBR_RS12460 at 545.096 kb on + strand, within BBR_RS12460 at 545.096 kb on + strand, within BBR_RS12460 at 545.122 kb on + strand, within BBR_RS12460 at 545.132 kb on + strand, within BBR_RS12460 at 545.137 kb on + strand, within BBR_RS12460 at 545.138 kb on - strand, within BBR_RS12460 at 545.177 kb on - strand, within BBR_RS12460 at 545.177 kb on - strand, within BBR_RS12460 at 545.186 kb on + strand, within BBR_RS12460 at 545.192 kb on - strand, within BBR_RS12460 at 545.268 kb on - strand, within BBR_RS12460 at 545.291 kb on + strand, within BBR_RS12460 at 545.303 kb on + strand, within BBR_RS12460 at 545.313 kb on + strand, within BBR_RS12460 at 545.318 kb on + strand, within BBR_RS12460 at 545.320 kb on + strand, within BBR_RS12460 at 545.338 kb on - strand, within BBR_RS12460 at 545.345 kb on - strand, within BBR_RS12460 at 545.371 kb on - strand, within BBR_RS12460 at 545.371 kb on - strand, within BBR_RS12460 at 545.375 kb on + strand, within BBR_RS12460 at 545.378 kb on + strand, within BBR_RS12460 at 545.419 kb on + strand, within BBR_RS12460 at 545.420 kb on + strand, within BBR_RS12460 at 545.469 kb on + strand, within BBR_RS12460 at 545.477 kb on + strand, within BBR_RS12460 at 545.479 kb on + strand, within BBR_RS12460 at 545.483 kb on + strand, within BBR_RS12460 at 545.547 kb on + strand, within BBR_RS12460 at 545.563 kb on + strand, within BBR_RS12460 at 545.563 kb on + strand, within BBR_RS12460
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Gramicidin 0.001 mM remove 542,753 + +0.6 542,760 - -1.5 542,760 - +0.1 542,771 - -0.4 542,779 + +0.0 542,789 + -0.3 542,796 - -0.8 542,796 - -0.6 542,798 + +1.6 542,821 + -0.2 542,821 + -0.9 542,848 + +0.5 542,850 + -0.9 542,850 + +0.1 542,856 - +1.0 542,875 + +1.6 542,875 + -1.5 543,000 + BBR_RS12450 0.23 +0.4 543,017 + BBR_RS12450 0.25 +0.2 543,036 + BBR_RS12450 0.27 -0.5 543,102 - BBR_RS12450 0.34 +0.0 543,104 + BBR_RS12450 0.34 +0.2 543,603 + BBR_RS12450 0.88 +0.9 543,667 - -0.7 543,669 + -0.1 543,669 + -0.5 543,669 + +0.2 543,669 + +0.0 543,680 + -0.7 543,752 + +0.6 543,754 - -0.4 543,793 - -1.7 543,834 - BBR_RS12455 0.11 -0.9 543,964 + BBR_RS12455 0.26 -0.6 543,964 + BBR_RS12455 0.26 +0.5 543,964 + BBR_RS12455 0.26 +0.7 543,964 + BBR_RS12455 0.26 +0.4 543,964 + BBR_RS12455 0.26 +0.1 543,982 - BBR_RS12455 0.28 +0.4 543,996 + BBR_RS12455 0.30 +0.2 543,996 + BBR_RS12455 0.30 +0.1 543,996 + BBR_RS12455 0.30 -1.0 544,065 + BBR_RS12455 0.38 +0.2 544,065 + BBR_RS12455 0.38 +0.6 544,073 - BBR_RS12455 0.39 -0.7 544,073 - BBR_RS12455 0.39 -1.7 544,073 - BBR_RS12455 0.39 -1.3 544,073 - BBR_RS12455 0.39 -1.7 544,082 - BBR_RS12455 0.40 +0.5 544,082 - BBR_RS12455 0.40 -0.5 544,082 - BBR_RS12455 0.40 -0.1 544,084 + BBR_RS12455 0.40 -0.6 544,084 + BBR_RS12455 0.40 +0.0 544,092 - BBR_RS12455 0.41 -0.3 544,092 - BBR_RS12455 0.41 -0.7 544,101 + BBR_RS12455 0.42 -0.1 544,101 + BBR_RS12455 0.42 +0.6 544,109 - BBR_RS12455 0.43 -1.3 544,136 + BBR_RS12455 0.46 -0.4 544,138 - BBR_RS12455 0.47 -0.5 544,150 - BBR_RS12455 0.48 +0.0 544,154 - BBR_RS12455 0.48 -0.6 544,164 - BBR_RS12455 0.50 -0.3 544,170 + BBR_RS12455 0.50 +0.0 544,176 + BBR_RS12455 0.51 +0.8 544,178 - BBR_RS12455 0.51 -0.4 544,181 + BBR_RS12455 0.52 -0.2 544,181 + BBR_RS12455 0.52 -0.1 544,187 + BBR_RS12455 0.52 +0.4 544,224 - BBR_RS12455 0.57 -0.4 544,232 + BBR_RS12455 0.57 +0.2 544,232 + BBR_RS12455 0.57 -0.9 544,232 + BBR_RS12455 0.57 -0.1 544,232 + BBR_RS12455 0.57 +0.5 544,232 + BBR_RS12455 0.57 +0.2 544,232 + BBR_RS12455 0.57 -0.0 544,254 - BBR_RS12455 0.60 -0.8 544,256 - BBR_RS12455 0.60 -0.7 544,256 - BBR_RS12455 0.60 -0.8 544,266 + BBR_RS12455 0.61 -0.1 544,272 + BBR_RS12455 0.62 +0.0 544,301 + BBR_RS12455 0.66 +0.6 544,324 + BBR_RS12455 0.68 -0.3 544,326 - BBR_RS12455 0.68 -0.3 544,334 + BBR_RS12455 0.69 +1.1 544,337 - BBR_RS12455 0.70 -1.1 544,380 + BBR_RS12455 0.75 +0.6 544,380 + BBR_RS12455 0.75 +0.1 544,380 + BBR_RS12455 0.75 +0.1 544,381 + BBR_RS12455 0.75 +0.2 544,388 - BBR_RS12455 0.76 +0.3 544,395 + BBR_RS12455 0.76 +0.3 544,398 - BBR_RS12455 0.77 -0.9 544,403 - BBR_RS12455 0.77 +1.6 544,415 + BBR_RS12455 0.79 +0.2 544,425 - BBR_RS12455 0.80 +0.8 544,448 + BBR_RS12455 0.83 -0.8 544,460 + BBR_RS12455 0.84 -0.3 544,460 + BBR_RS12455 0.84 -0.1 544,466 - BBR_RS12455 0.85 -0.5 544,499 - BBR_RS12455 0.89 -0.4 544,503 - BBR_RS12455 0.89 -0.3 544,506 + BBR_RS12455 0.89 +0.3 544,512 + +0.2 544,521 - -0.0 544,521 - -1.1 544,554 - +0.7 544,564 + +2.4 544,570 - +1.2 544,570 - +0.5 544,598 - -0.9 544,608 + -1.0 544,608 + +0.3 544,611 - -0.3 544,624 + -0.4 544,713 - +0.8 544,725 + +0.8 544,739 + +0.1 544,746 + -0.0 544,805 + +0.2 544,833 + -1.2 544,833 + -0.1 544,841 - -0.4 544,866 + +0.0 544,866 - -2.6 544,868 + -0.3 544,868 + -1.9 544,895 - BBR_RS12460 0.12 -1.7 544,900 + BBR_RS12460 0.13 -1.0 544,918 - BBR_RS12460 0.14 -1.3 544,918 - BBR_RS12460 0.14 -0.4 544,933 - BBR_RS12460 0.16 -0.8 544,955 - BBR_RS12460 0.18 -0.2 544,995 - BBR_RS12460 0.21 -1.2 545,044 + BBR_RS12460 0.26 -0.8 545,044 + BBR_RS12460 0.26 -1.3 545,052 - BBR_RS12460 0.26 -0.6 545,054 + BBR_RS12460 0.27 -1.2 545,083 - BBR_RS12460 0.29 +2.3 545,096 + BBR_RS12460 0.30 -0.1 545,096 + BBR_RS12460 0.30 -0.2 545,122 + BBR_RS12460 0.33 -1.0 545,132 + BBR_RS12460 0.34 -1.0 545,137 + BBR_RS12460 0.34 -0.3 545,138 - BBR_RS12460 0.34 +0.3 545,177 - BBR_RS12460 0.38 -0.4 545,177 - BBR_RS12460 0.38 -1.0 545,186 + BBR_RS12460 0.38 +1.0 545,192 - BBR_RS12460 0.39 +0.4 545,268 - BBR_RS12460 0.46 -1.4 545,291 + BBR_RS12460 0.48 -0.7 545,303 + BBR_RS12460 0.49 +0.4 545,313 + BBR_RS12460 0.50 -0.6 545,318 + BBR_RS12460 0.50 -0.0 545,320 + BBR_RS12460 0.50 +0.8 545,338 - BBR_RS12460 0.52 -0.7 545,345 - BBR_RS12460 0.53 +0.0 545,371 - BBR_RS12460 0.55 -0.8 545,371 - BBR_RS12460 0.55 -0.2 545,375 + BBR_RS12460 0.55 -0.2 545,378 + BBR_RS12460 0.56 +0.0 545,419 + BBR_RS12460 0.59 -0.7 545,420 + BBR_RS12460 0.59 -0.7 545,469 + BBR_RS12460 0.64 -1.1 545,477 + BBR_RS12460 0.64 +1.0 545,479 + BBR_RS12460 0.65 -1.3 545,483 + BBR_RS12460 0.65 -2.1 545,547 + BBR_RS12460 0.71 +1.0 545,563 + BBR_RS12460 0.72 +1.8 545,563 + BBR_RS12460 0.72 -0.4
Or see this region's nucleotide sequence