Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2252 and Rru_A2253 are separated by 12 nucleotides Rru_A2253 and Rru_A2254 are separated by 12 nucleotides Rru_A2254 and Rru_A2255 are separated by 146 nucleotides
Rru_A2252: Rru_A2252 - Amino acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine (NCBI), at 2,614,498 to 2,615,196
_A2252
Rru_A2253: Rru_A2253 - Amino acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine (NCBI), at 2,615,209 to 2,615,928
_A2253
Rru_A2254: Rru_A2254 - hypothetical protein (NCBI), at 2,615,941 to 2,616,486
_A2254
Rru_A2255: Rru_A2255 - Uracil phosphoribosyl transferase (NCBI), at 2,616,633 to 2,617,289
_A2255
Position (kb)
2615
2616
2617 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2615.074 kb on + strand, within Rru_A2252 at 2615.074 kb on + strand, within Rru_A2252 at 2615.075 kb on - strand, within Rru_A2252 at 2615.075 kb on - strand, within Rru_A2252 at 2615.075 kb on - strand, within Rru_A2252 at 2615.075 kb on - strand, within Rru_A2252 at 2615.075 kb on - strand, within Rru_A2252 at 2615.107 kb on + strand, within Rru_A2252 at 2615.107 kb on + strand, within Rru_A2252 at 2615.108 kb on - strand, within Rru_A2252 at 2615.371 kb on + strand, within Rru_A2253 at 2615.429 kb on - strand, within Rru_A2253 at 2615.429 kb on - strand, within Rru_A2253 at 2615.540 kb on - strand, within Rru_A2253 at 2615.608 kb on + strand, within Rru_A2253 at 2615.609 kb on - strand, within Rru_A2253 at 2615.745 kb on + strand, within Rru_A2253 at 2615.770 kb on + strand, within Rru_A2253 at 2615.771 kb on - strand, within Rru_A2253 at 2615.771 kb on - strand, within Rru_A2253 at 2615.771 kb on - strand, within Rru_A2253 at 2615.771 kb on - strand, within Rru_A2253 at 2615.779 kb on + strand, within Rru_A2253 at 2615.779 kb on + strand, within Rru_A2253 at 2615.782 kb on + strand, within Rru_A2253 at 2615.782 kb on + strand, within Rru_A2253 at 2615.782 kb on + strand, within Rru_A2253 at 2615.782 kb on + strand, within Rru_A2253 at 2615.783 kb on - strand, within Rru_A2253 at 2615.821 kb on + strand, within Rru_A2253 at 2615.821 kb on + strand, within Rru_A2253 at 2615.821 kb on + strand, within Rru_A2253 at 2615.821 kb on + strand, within Rru_A2253 at 2615.822 kb on - strand, within Rru_A2253 at 2615.822 kb on - strand, within Rru_A2253 at 2615.822 kb on - strand, within Rru_A2253 at 2615.836 kb on + strand, within Rru_A2253 at 2615.837 kb on - strand, within Rru_A2253 at 2615.995 kb on + strand at 2615.996 kb on - strand, within Rru_A2254 at 2615.996 kb on - strand, within Rru_A2254 at 2615.996 kb on - strand, within Rru_A2254 at 2616.022 kb on + strand, within Rru_A2254 at 2616.022 kb on + strand, within Rru_A2254 at 2616.023 kb on - strand, within Rru_A2254 at 2616.111 kb on - strand, within Rru_A2254 at 2616.242 kb on + strand, within Rru_A2254 at 2616.242 kb on + strand, within Rru_A2254 at 2616.243 kb on - strand, within Rru_A2254 at 2616.243 kb on - strand, within Rru_A2254 at 2616.243 kb on - strand, within Rru_A2254 at 2616.635 kb on - strand at 2616.635 kb on - strand at 2616.724 kb on + strand, within Rru_A2255 at 2616.725 kb on - strand, within Rru_A2255 at 2616.725 kb on - strand, within Rru_A2255 at 2616.725 kb on - strand, within Rru_A2255 at 2616.959 kb on - strand, within Rru_A2255 at 2617.141 kb on + strand, within Rru_A2255 at 2617.142 kb on - strand, within Rru_A2255 at 2617.211 kb on + strand, within Rru_A2255 at 2617.212 kb on - strand, within Rru_A2255 at 2617.212 kb on - strand, within Rru_A2255 at 2617.212 kb on - strand, within Rru_A2255 at 2617.212 kb on - strand, within Rru_A2255 at 2617.329 kb on + strand at 2617.329 kb on + strand at 2617.329 kb on + strand at 2617.329 kb on + strand at 2617.330 kb on - strand at 2617.330 kb on - strand at 2617.437 kb on + strand at 2617.437 kb on + strand at 2617.437 kb on + strand at 2617.438 kb on - strand at 2617.438 kb on - strand at 2617.441 kb on + strand at 2617.441 kb on + strand at 2617.441 kb on + strand at 2617.442 kb on - strand at 2617.442 kb on - strand at 2617.442 kb on - strand at 2617.442 kb on - strand at 2617.442 kb on - strand at 2617.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 2,615,074 + Rru_A2252 0.82 +0.4 2,615,074 + Rru_A2252 0.82 -4.5 2,615,075 - Rru_A2252 0.83 +0.1 2,615,075 - Rru_A2252 0.83 +0.1 2,615,075 - Rru_A2252 0.83 -0.9 2,615,075 - Rru_A2252 0.83 +0.6 2,615,075 - Rru_A2252 0.83 -0.6 2,615,107 + Rru_A2252 0.87 +0.3 2,615,107 + Rru_A2252 0.87 -1.3 2,615,108 - Rru_A2252 0.87 -0.3 2,615,371 + Rru_A2253 0.23 +0.2 2,615,429 - Rru_A2253 0.31 +0.2 2,615,429 - Rru_A2253 0.31 +0.8 2,615,540 - Rru_A2253 0.46 -2.9 2,615,608 + Rru_A2253 0.55 -0.1 2,615,609 - Rru_A2253 0.56 +0.1 2,615,745 + Rru_A2253 0.74 -0.4 2,615,770 + Rru_A2253 0.78 -5.1 2,615,771 - Rru_A2253 0.78 +1.7 2,615,771 - Rru_A2253 0.78 +0.3 2,615,771 - Rru_A2253 0.78 +1.3 2,615,771 - Rru_A2253 0.78 -0.0 2,615,779 + Rru_A2253 0.79 -0.5 2,615,779 + Rru_A2253 0.79 -0.9 2,615,782 + Rru_A2253 0.80 +1.3 2,615,782 + Rru_A2253 0.80 +0.5 2,615,782 + Rru_A2253 0.80 -2.7 2,615,782 + Rru_A2253 0.80 +0.9 2,615,783 - Rru_A2253 0.80 -0.0 2,615,821 + Rru_A2253 0.85 -0.3 2,615,821 + Rru_A2253 0.85 -0.0 2,615,821 + Rru_A2253 0.85 -1.1 2,615,821 + Rru_A2253 0.85 -2.0 2,615,822 - Rru_A2253 0.85 +0.8 2,615,822 - Rru_A2253 0.85 +1.5 2,615,822 - Rru_A2253 0.85 +0.4 2,615,836 + Rru_A2253 0.87 -0.2 2,615,837 - Rru_A2253 0.87 +0.4 2,615,995 + -0.9 2,615,996 - Rru_A2254 0.10 -0.5 2,615,996 - Rru_A2254 0.10 -0.0 2,615,996 - Rru_A2254 0.10 -1.9 2,616,022 + Rru_A2254 0.15 -0.4 2,616,022 + Rru_A2254 0.15 +0.9 2,616,023 - Rru_A2254 0.15 -0.6 2,616,111 - Rru_A2254 0.31 -0.8 2,616,242 + Rru_A2254 0.55 -0.7 2,616,242 + Rru_A2254 0.55 +0.9 2,616,243 - Rru_A2254 0.55 -3.4 2,616,243 - Rru_A2254 0.55 +1.2 2,616,243 - Rru_A2254 0.55 -0.4 2,616,635 - +2.2 2,616,635 - -0.3 2,616,724 + Rru_A2255 0.14 +0.1 2,616,725 - Rru_A2255 0.14 -2.5 2,616,725 - Rru_A2255 0.14 +0.8 2,616,725 - Rru_A2255 0.14 +0.3 2,616,959 - Rru_A2255 0.50 -0.3 2,617,141 + Rru_A2255 0.77 +1.7 2,617,142 - Rru_A2255 0.77 +0.7 2,617,211 + Rru_A2255 0.88 -0.8 2,617,212 - Rru_A2255 0.88 +0.4 2,617,212 - Rru_A2255 0.88 +0.4 2,617,212 - Rru_A2255 0.88 -0.2 2,617,212 - Rru_A2255 0.88 +1.5 2,617,329 + -1.8 2,617,329 + -2.0 2,617,329 + +0.2 2,617,329 + +0.5 2,617,330 - -1.4 2,617,330 - -1.9 2,617,437 + -0.1 2,617,437 + -3.2 2,617,437 + +0.5 2,617,438 - +0.7 2,617,438 - +0.1 2,617,441 + +0.5 2,617,441 + -0.7 2,617,441 + -3.7 2,617,442 - -1.0 2,617,442 - -1.6 2,617,442 - +1.5 2,617,442 - +0.4 2,617,442 - +0.9 2,617,458 - -0.1
Or see this region's nucleotide sequence