Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1164 and Rru_A1165 are separated by 17 nucleotides Rru_A1165 and Rru_A1166 are separated by 16 nucleotides Rru_A1166 and Rru_A1167 overlap by 4 nucleotides
Rru_A1163: Rru_A1163 - Nickel-dependent hydrogenase b-type cytochrome subunit (NCBI), at 1,381,364 to 1,382,083
_A1163
Rru_A1164: Rru_A1164 - Peptidase M52, hydrogenase expression/formation protein (NCBI), at 1,382,080 to 1,382,676
_A1164
Rru_A1165: Rru_A1165 - Hydrogenase expression/formation protein (HUPF/HYPC) (NCBI), at 1,382,694 to 1,383,065
_A1165
Rru_A1166: Rru_A1166 - hydrogenase expression/formation protein hupH (NCBI), at 1,383,082 to 1,383,858
_A1166
Rru_A1167: Rru_A1167 - putative hydrogenase expression/formation protein HupK (NCBI), at 1,383,855 to 1,385,075
_A1167
Position (kb)
1383
1384 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1382.225 kb on + strand, within Rru_A1164 at 1382.226 kb on - strand, within Rru_A1164 at 1382.270 kb on - strand, within Rru_A1164 at 1382.662 kb on + strand at 1382.693 kb on + strand at 1382.693 kb on + strand at 1382.693 kb on + strand at 1382.694 kb on - strand at 1382.694 kb on - strand at 1382.730 kb on - strand at 1383.165 kb on + strand, within Rru_A1166 at 1383.165 kb on + strand, within Rru_A1166 at 1383.166 kb on - strand, within Rru_A1166 at 1383.166 kb on - strand, within Rru_A1166 at 1383.166 kb on - strand, within Rru_A1166 at 1383.166 kb on - strand, within Rru_A1166 at 1383.166 kb on - strand, within Rru_A1166 at 1383.359 kb on + strand, within Rru_A1166 at 1383.360 kb on - strand, within Rru_A1166 at 1383.828 kb on - strand at 1384.745 kb on + strand, within Rru_A1167 at 1384.746 kb on - strand, within Rru_A1167
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 1,382,225 + Rru_A1164 0.24 +1.3 1,382,226 - Rru_A1164 0.24 -3.3 1,382,270 - Rru_A1164 0.32 +1.8 1,382,662 + +0.6 1,382,693 + -0.2 1,382,693 + +1.7 1,382,693 + +0.0 1,382,694 - -0.1 1,382,694 - +1.1 1,382,730 - +0.2 1,383,165 + Rru_A1166 0.11 +0.2 1,383,165 + Rru_A1166 0.11 -1.3 1,383,166 - Rru_A1166 0.11 -1.8 1,383,166 - Rru_A1166 0.11 -0.7 1,383,166 - Rru_A1166 0.11 +0.1 1,383,166 - Rru_A1166 0.11 +0.1 1,383,166 - Rru_A1166 0.11 -0.2 1,383,359 + Rru_A1166 0.36 -0.9 1,383,360 - Rru_A1166 0.36 +0.3 1,383,828 - +0.7 1,384,745 + Rru_A1167 0.73 +1.0 1,384,746 - Rru_A1167 0.73 -0.6
Or see this region's nucleotide sequence